Kcnh1 (NM_010600) Mouse Untagged Clone

CAT#: MC222991

Kcnh1 (untagged) - Mouse potassium voltage-gated channel, subfamily H (eag-related), member 1 (Kcnh1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnh1
• Synonyms: EAG1; Kv10.1; M-eag
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222991 representing NM_010600
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGGCTGGCGGCCGGCGGGGACTAGTGGCCCCGCAGAACACGTTTCTGGAGAACATCGTGCGGC
  GGTCCAACGACACTAATTTTGTGTTGGGGAATGCCCAGATAGTCGACTGGCCCATCGTGTACAGCAATGA
  TGGATTCTGCAAGCTGTCTGGCTACCACCGGGCAGAAGTGATGCAGAAAAGCAGCGCCTGCAGTTTTATG
  TATGGAGAGCTGACTGACAAGGACACAGTTGAAAAGGTTCGCCAGACCTTTGAGAACTACGAGATGAATT
  CCTTCGAAATTCTGATGTACAAGAAGAACAGGACACCTGTGTGGTTTTTTGTGAAGATCGCTCCGATCAG
  GAACGAACAGGATAAAGTGGTCCTGTTCCTTTGCACTTTCAGCGACATAACTGCATTCAAGCAGCCCATC
  GAGGACGACTCCTGCAAAGGTTGGGGGAAGTTTGCCCGACTGACCAGAGCTCTGACAAGCAGCAGGGGAG
  TCCTGCAGCAGCTGGCCCCCAGTGTACAGAAGGGTGAGAATGTTCACAAGCACTCGCGCCTGGCAGAGGT
  CCTGCAGCTGGGTTCAGACATCCTTCCCCAGTACAAGCAAGAGGCGCCAAAGACACCCCCTCACATCATC
  CTACACTACTGTGTCTTTAAGACCACGTGGGATTGGATCATCTTGATCCTGACCTTCTACACAGCCATCT
  TGGTCCCTTACAACGTCTCCTTTAAAACCAGGCAGAATAATGTGGCCTGGCTGGTGGTGGACAGCATCGT
  GGATGTCATCTTTTTGGTGGACATTGTTTTGAATTTTCACACCACCTTTGTCGGGCCAGCGGGGGAAGTG
  ATATCTGACCCCAAACTTATCCGCATGAACTACCTGAAGACGTGGTTTGTGATCGACCTTCTGTCCTGTT
  TGCCGTATGACGTCATCAACGCTTTTGAGAACGTGGATGAGGTTAGTGCCTTTATGGGTGATCCAGGGAA
  GATTGGTTTTGCTGATCAGATCCCACCACCACTGGAGGGGAGAGAGAGTCAGGGCATCAGCAGCCTGTTC
  AGTTCTCTGAAAGTTGTGCGGCTGCTCCGTCTCGGGCGAGTGGCCCGCAAACTGGATCATTACATCGAGT
  ATGGAGCCGCGGTACTGGTCCTGTTGGTGTGCGTGTTTGGGCTTGCTGCCCACTGGATGGCCTGCATCTG
  GTACAGCATTGGGGACTATGAGATCTTTGATGAAGACACCAAGACCATCCGCAACAACAGCTGGCTCTAC
  CAACTGGCATTGGACATCGGCACTCCATACCAGTTTAATGGGTCTGGTTCAGGGAAGTGGGAAGGTGGGC
  CAAGCAAGAACTCTGTCTACATCTCCTCGCTGTACTTCACCATGACAAGTCTCACCAGTGTGGGCTTTGG
  TAACATTGCCCCATCCACAGACATCGAGAAGATCTTCGCAGTGGCCATCATGATGATTGGCTCTCTTCTG
  TATGCCACCATCTTTGGGAACGTGACAACCATTTTCCAGCAGATGTACGCCAACACCAACAGGTACCACG
  AGATGCTCAACAGTGTCCGGGATTTCCTGAAGCTCTACCAGGTGCCCAAAGGGCTGAGTGAGCGGGTCAT
  GGACTACATTGTGTCCACCTGGTCCATGTCCCGAGGCATCGACACAGAGAAGGTCCTGCAGATCTGCCCC
  AAGGACATGCGAGCCGACATTTGCGTGCACCTGAACCGCAAAGTGTTCAAAGAACACCCTGCCTTTCGAT
  TGGCCAGCGATGGCTGCCTGCGGGCCTTGGCCATGGAGTTCCAGACAGTGCACTGTGCCCCAGGGGACCT
  CATCTATCATGCTGGAGAGAGTGTGGACAGCCTCTGCTTCGTGGTCTCAGGCTCCCTGGAGGTGATCCAG
  GATGACGAGGTGGTGGCCATCCTAGGGAAAGGAGACGTGTTTGGGGATGTTTTCTGGAAGGAAGCTACCC
  TTGCACAGTCCTGTGCTAATGTCCGGGCCTTGACCTACTGTGACCTGCACGTGATCAAGAGGGACGCCCT
  TCAGAAAGTGCTAGAATTCTACACAGCCTTCTCCCACTCCTTCTCCCGGAACCTGATTCTCACCTACAAC
  CTGAGGAAGAGGATTGTGTTCCGGAAGATCAGCGACGTGAAACGAGAAGAGGAGGAGAGGATGAAACGGA
  AGAACGAGGCCCCCCTTATCCTGCCTCCTGACCACCCCGTCAGAAGACTCTTCCAAAGGTTCCGCCAGCA
  GAAAGAAGCCAGGCTGGCAGCCGAGAGAGGGGGCCGGGACCTGGATGACCTGGATGTAGAAAAGGGCAAT
  GCCCTCACGGACCATACCTCAGCCAACCACAGCCTGGTGAAGGCCAGTGTGGTCACGGTGCGTGAGAGCC
  CCGCCACGCCTGTGTCCTTCCAGGCGGCCACCACCTCCACCATGTCAGACCACGCCAAGCTGCATGCCCC
  CGGATCTGAGTGCCTAGGTCCCAAGGCAGTCAGCTGTGACCCTGCCAAGCGCAAAGGCTGGGCCCGTTTC
  AAAGATGCCTGTGGAAAGGGTGAGGATTGGAACAAGGTGTCCAAGGCAGAGTCCATGGAGACGCTGCCCG
  AGAGGACAAAGGCACCGGGTGAGGCCACGCTGAAGAAGACAGACTCCTGTGACAGCGGAATCACCAAGAG
  CGACCTGCGCTTGGACAATGTGGGTGAGACCAGGAGTCCTCAGGACCGGAGCCCCATCTTGGCTGAGGTC
  AAGCATTCTTTCTACCCCATCCCTGAGCAGACGCTGCAGGCCACAGTGCTGGAGGTGAAATATGAGCTGA
  AGGAGGATATCAAGGCCTTGAATGCCAAAATGACCTCCATTGAGAAGCAGCTGTCTGAGATACTCAGGAT
  ACTAATGTCCAGAGGGTCCGCCCAATCTCCTCAGGAGACGGGTGAGATCTCCAGGCCCCAGTCCCCAGAG
  TCAGACAGAGACATTTTTGGAGCAAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010600
• Insert Size: 2970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010600.3, NP_034730.1
• RefSeq Size: 7269 bp
• RefSeq ORF: 2970 bp
• Locus ID: 16510
• UniProt ID: Q60603
• Cytogenetics: 1 H6
• Gene Summary: Pore-forming (alpha) subunit of a voltage-gated delayed rectifier potassium channel (PubMed:19671703, PubMed:23975098). Channel properties are modulated by subunit assembly. Mediates IK(NI) current in myoblasts. Involved in the regulation of cell proliferation and differentiation, in particular adipogenic and osteogenic differentiation in bone marrow-derived mesenchymal stem cells (MSCs) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).