Atp2a1 (NM_007504) Mouse Untagged Clone

CAT#: MC222982

Atp2a1 (untagged) - Mouse ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 (Atp2a1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp2a1
• Synonyms: SERCA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222982 representing NM_007504
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCGCGCACTCCAAGTCCACAGAGGAATGTTTGTCCTATTTCGGGGTGAGCGAGACCACAGGCC
  TTACCCCAGACCAAGTTAAGCGACATCTGGAAAAATACGGCCCCAATGAGCTCCCTGCTGAGGAAGGCAA
  GTCTCTGTGGGAGTTGGTGGTAGAGCAGTTCGAAGACCTGCTTGTGCGGATTCTTCTCCTGGCTGCCTGC
  ATTTCCTTTGTGCTGGCCTGGTTTGAGGAAGGCGAAGAAACCGTCACTGCCTTTGTGGAACCTTTTGTCA
  TTCTCTTGATCCTCATTGCCAACGCCATTGTGGGGGTTTGGCAGGAACGGAATGCAGAGAACGCTATCGA
  AGCGCTGAAAGAATATGAACCCGAGATGGGAAAGGTCTATCGGGCTGACCGAAAGTCAGTGCAAAGGATC
  AAGGCTCGGGACATCGTTCCTGGGGACATCGTGGAGGTGGCCGTTGGGGACAAAGTCCCTGCAGACATCC
  GCATCCTCTCCATCAAATCCACCACCCTCCGGGTCGACCAGTCCATCCTGACAGGAGAGTCTGTGTCTGT
  CATCAAGCACACAGACCCTGTCCCTGACCCCCGAGCTGTCAACCAGGACAAGAAGAACATGCTCTTCTCA
  GGTACCAACATCGCAGCCGGCAAGGCTGTAGGCATCGTGGCCACCACTGGTGTGAGTACTGAGATTGGCA
  AGATCCGAGACCAGATGGCTGCCACCGAGCAGGACAAGACTCCACTGCAGCAGAAGCTTGACGAGTTTGG
  GGAGCAGCTGTCCAAGGTCATCTCTCTCATCTGTGTGGCTGTCTGGCTTATCAACATTGGCCACTTCAAC
  GATCCTGTCCATGGGGGCTCCTGGTTCCGGGGTGCCATTTACTACTTCAAGATTGCTGTGGCCTTGGCTG
  TGGCGGCCATCCCAGAAGGGCTTCCTGCTGTCATCACCACCTGCTTGGCTTTGGGTACCCGCCGGATGGC
  AAAGAAGAACGCCATCGTGAGGAGTCTGCCCTCTGTGGAGACCCTGGGCTGTACCTCTGTCATCTGTTCT
  GACAAAACAGGGACCCTCACCACCAACCAGATGTCAGTTTGCAAGATGTTCATCATTGACAAGGTGGATG
  GGGATGTCTGCTCACTGAACGAGTTCTCCATCACTGGCTCTACTTATGCTCCTGAGGGAGAGGTCTTGAA
  GAATGATAAGCCAGTCCGAGCAGGGCAGTATGATGGGCTGGTGGAGCTCGCTACCATCTGTGCCCTCTGC
  AATGATTCCTCCTTGGACTTTAATGAGACCAAAGGTGTCTATGAGAAGGTGGGCGAGGCCACGGAGACTG
  CTCTCACCACCTTGGTGGAGAAGATGAACGTATTCAACACGGAAGTGAGAAGCCTCTCTAAAGTGGAGCG
  GGCCAACGCCTGCAATTCGGTGATCCGCCAACTCATGAAGAAGGAATTCACCCTGGAATTTTCCCGAGAC
  AGGAAGTCTATGTCTGTCTACTGTTCACCAGCTAAGTCCTCACGGGCTGCCGTGGGCAACAAGATGTTTG
  TCAAGGGTGCCCCCGAGGGGGTCATCGACCGCTGTAACTATGTGCGTGTTGGCACGACCCGTGTGCCATT
  GACAGGCCCCGTGAAGGAGAAGATCATGTCTGTGATCAAGGAGTGGGGCACTGGTCGGGACACACTGCGG
  TGCCTGGCGCTGGCCACCCGCGACACGCCCCCCAAACGCGAGGAGATGGTTCTGGATGATTCAGCCAAGT
  TCATGGAGTATGAGATGGACCTGACGTTTGTTGGTGTTGTGGGCATGTTGGATCCACCCCGCAAGGAGGT
  CACAGGCTCTATCCAGCTGTGTCGTGATGCTGGGATCCGAGTGATCATGATCACTGGGGACAACAAGGGT
  ACAGCCATTGCCATCTGCCGACGAATTGGCATCTTTTCGGAGAATGAGGAAGTGACAGACCGTGCCTACA
  CTGGCCGTGAGTTTGACGATCTGCCCCTGGCCGAGCAGCGGGAGGCCTGCAGACGCGCCTGCTGCTTTGC
  TCGTGTGGAGCCCTCACACAAGTCCAAGATTGTGGAGTACCTGCAGTCCTACGATGAGATCACAGCCATG
  ACAGGGGATGGTGTCAATGATGCCCCTGCTCTGAAGAAGGCCGAGATTGGCATTGCCATGGGATCTGGTA
  CTGCTGTGGCTAAGACGGCCTCTGAAATGGTACTGGCCGATGATAACTTCTCCACCATCGTGGCTGCCGT
  GGAGGAAGGCCGCGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCCTCCAATGTGGGC
  GAGGTGGTCTGTATCTTCTTGACAGCAGCCTTGGGGCTGCCTGAGGCTCTGATCCCTGTGCAGCTGCTCT
  GGGTGAACTTGGTGACTGATGGGCTCCCGGCTACTGCCCTGGGATTCAACCCACCTGACCTGGACATCAT
  GGACCGCCCCCCCAGGAGTCCCAAGGAGCCTCTTATCAGTGGCTGGCTCTTTTTCCGCTACATGGCAATT
  GGGGGCTATGTGGGTGCAGCCACTGTAGGAGCAGCTGCCTGGTGGTTTTTGTATGCAGAGGACGGGCCTC
  ATGTCAGCTACCATCAGCTGACTCATTTCATGCAGTGCACTGAGCACAACCCTGAATTCGATGGCCTGGA
  CTGTGAGGTCTTTGAGGCCCCCGAGCCCATGACCATGGCCTTGTCTGTGTTGGTGACCATCGAGATGTGC
  AATGCTCTCAACAGCCTGTCTGAGAACCAGTCCCTACTGCGGATGCCACCCTGGGTGAACATCTGGCTGC
  TGGGTTCCATCTGCCTGTCCATGTCCCTCCACTTCCTCATCCTCTATGTCGACCCCCTGCCGATGATCTT
  CAAGCTCCGGGCCCTGGACTTTACCCAGTGGCTCATGGTCCTCAAGATCTCACTGCCAGTCATCGGGCTG
  GATGAGCTTCTCAAGTTCATTGCTCGGAACTATCTGGAGGGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007504
• Insert Size: 2985 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC036292, AAH36292
• RefSeq Size: 3486 bp
• RefSeq ORF: 2985 bp
• Locus ID: 11937
• UniProt ID: Q8R429
• Cytogenetics: 7 69.04 cM
• Gene Summary: Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum (PubMed:21697544, PubMed:22961106, PubMed:25640239, PubMed:26816378). Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed:21697544, PubMed:22961106, PubMed:25640239, PubMed:26816378).[UniProtKB/Swiss-Prot Function]