Nek9 (NM_145138) Mouse Untagged Clone

CAT#: MC222965

Nek9 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nek9
• Synonyms: C130021H08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222965 representing NM_145138
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTCCATCAACTCGGACTTCGGAAGCGAGTCCGGGG
  GTGGCGGGGACTCGGGCCCGGGCCCCAGCGCTGTCCCAGGGCCGCGAGCCGGCGGCGGGGCGGCGGAGCA
  GGAGGAGCTGCACTACATCCCCATCCGCGTTCTGGGCCGCGGTGCCTTTGGGGAGGCCACGCTGTACCGC
  CGCACCGAGGACGACTCACTGGTGGTGTGGAAGGAAGTTGACTTGACCCGGCTATCTGAGAAGGAACGTC
  GAGATGCCCTGAATGAGATTGTCATCCTGGCACTGCTACAGCACGACAACATCATTGCCTACTACAACCA
  CTTCATGGACAACACCACCCTGCTGATTGAGCTGGAGTACTGTAACGGAGGGAACCTCTATGACAAAATC
  CTTCGTCAGAAGGACAAGTTGTTCGAAGAAGAGATGGTGGTGTGGTACCTTTTTCAGATTGTTTCAGCAG
  TGAGCTGTATTCATAAAGCTGGAATCCTCCATAGAGATATAAAGACATTAAATATTTTTCTGACCAAGGC
  AAATCTGATAAAGCTTGGAGACTATGGCCTAGCAAAGAAACTTAATTCTGAGTACTCTATGGCCGAGACA
  CTTGTGGGCACTCCATATTACATGTCTCCAGAGCTCTGCCAAGGAGTAAAATACAATTTCAAGTCTGATA
  TATGGGCAGTTGGCTGTGTCATTTTTGAACTGCTTACTCTGAAAAGAACATTTGATGCTACAAACCCACT
  CAACCTATGTGTGAAGATCGTGCAGGGAATCCGGGCCATGGAAGTTGACTCGAGTCAGTATTCTTTGGAA
  CTGATCCAGTTGGTACACGCATGCCTCGACCAGGATCCTGAGCAGAGGCCCGCTGCAGATGCACTCCTGG
  ATCTCCCCCTTCTCAGGACCCGCAGGAGAGAGATGGAGGAAAAAGTCACTCTGCTCAATGCACCTACAAA
  GAGACCAAGGTCAAGCACTGTGACGGAAGCACCCATTGCTGTGGTAACATCACGAACCAGTGAAGTCTAT
  GTTTGGGGTGGTGGAAAATCCACACCTCAGAAATTGGACGTCATCAAGAGTGGCTGTAGTGCTCGACAGG
  TGTGTGCGGGGAACACCCACTTTGCTGTGGTCACCGTGGAGAAGGAACTATATACCTGGGTGAACATGCA
  AGGGGGCACTAAACTTCACGGTCAGCTAGGACATGGAGACAAAGCTTCCTACCGACAGCCAAAGCATGTA
  GAAAAGTTACAAGGGAAGGCTATCCACCAGGTGTCGTGTGGGGATGATTTCACTGTCTGTGTCACAGATG
  AGGGTCAGCTCTACGCCTTTGGATCAGATTATTATGGCTGCATGGGAGTGGACAAGGTTTCTGGCCCTGA
  AGTGCTAGAACCCATGCAGCTGAACTTCTTCCTCAGCAATCCTGTGGAGCAGGTCTCCTGTGGGGATAAT
  CATGTGGTAGTTCTGACACGAAACAAAGAAGTCTACTCTTGGGGCTGTGGTGAATATGGACGGCTGGGTT
  TGGATTCAGAAGAGGATTACTATACACCACAGAGGGTGGATGTTCCAAAGGCCTTAATCATTGTTGCAGT
  TCAATGTGGCTGTGATGGGACCTTTCTGCTCACTCAGTCAGGCAAAGTGCTGGCCTGTGGACTCAATGAG
  TTCAACAAGCTGGGGCTGAATCAGTGCATGTCTGGAATCATCAACCATGAAGCATACCATGAAGTTCCCT
  ACACAACCTCCTTTACCTTGGCCAAACAGTTGTCCTTTTACAAAATCCGTACCATTGCCCCAGGCAAGAC
  TCACACAGCTGCTATTGATGAACGAGGCCGGTTGCTGACTTTTGGCTGTAATAAGTGTGGGCAGCTGGGT
  GTTGGGAATTATAAGAAACGCCTGGGAATCAACCTGTTGGGGGGACCCCTTGGCGGGAAGCAAGTGATTA
  GGGTCTCGTGTGGCGATGAGTTCACCATTGCTGCCACCGACGATAATCACATCTTTGCCTGGGGTAATGG
  TGGTAATGGCCGCCTGGCAATGACTCCCACAGAGAGACCACATGGCTCTGATATCTGTACCTCATGGCCT
  CGGCCTATTTTTGGATCCCTGCATCATGTTCCAGACCTTTCTTGCCGTGGCTGGCACACCATTCTTATTG
  TTGAGAAAGTGTTGAATTCCAAGACCATCCGTTCCAATAGCAGTGGCCTGTCCATCGGAACTGTGGTTCA
  GAGCTCCAGCCCAGGAGGTGGCATTGGTGGTGGTGGTGGTGGTGGCGGCGGCGGCGGGGGTGAAGAGGAA
  GACAGTCAACAGGAATCTGAAACTCCAGATCCAAGCGGAGGCTTCCGAGGAACAATGGAAGCAGACCGTG
  GGATGGAAGGCTTAATCAGTCCCACGGAGGCCGTGGGAAACAGCTGTGGGGCTAGCAGCTCCTGTCCTGG
  CTGGCTTCGGAAGGAGCTGGAAAACGCAGAGTTCATTCCCATGCCTGACAGCCCCGCTCCCTTAAGTGCG
  GCGTTTTCACAGTCTGAGAAGGACACGCTGCCCTATGAAGAGCTGCAGGGACTCAAGGTCGCATCTGAAG
  TTCCTCCGGAGCCCCAGCGTGCAGCAGGAGCCTGGCCCCCTCGGCTGGATCCTGCAGTGCCGTGTGTTGG
  GAAAGCCCTGACCTCTGCTGCTTGTGCGTGCAGTGCTCTGCAGGTGGAGGTTGACAGGTTGCAGGCTCTG
  GTGTTAAAGTGTCTGGAAGAACAGCAGAAGCTCCAGCAAGAAAACCTCCAGATGTTTACCCAACTACAAA
  AGCTCAACAAGAAATTGGAAGGAGGCCAGCAGGTGGGGATGCACTCCAGAGGAACCCAGACAGCAAAGGA
  AGAAATGGAAATGGATCCAAAGCCTGACTTAGACTCGGAGTCCTGGTGCCTCCTCGGAACAGACTCTTGT
  CGACCCAGCCTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145138
• Insert Size: 2955 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC117971, AAI17972
• RefSeq Size: 4152 bp
• RefSeq ORF: 2955 bp
• Locus ID: 217718
• UniProt ID: Q8K1R7
• Cytogenetics: 12 D1
• Gene Summary: Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively (By similarity).[UniProtKB/Swiss-Prot Function]