Nek9 (NM_145138) Mouse Tagged ORF Clone

CAT#: MG216306

Nek9 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), (10ug)

Product Images

Circular map for MG216306

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nek9
• Synonyms: C130021H08Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216306 representing NM_145138
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGTGCTGGGCGAGTACGAGCGACACTGCGATTCCATCAACTCGGACTTCGGAAGCGAGTCCGGGG
  GTGGCGGGGACTCGGGCCCGGGCCCCAGCGCTGTCCCAGGGCCGCGAGCCGGCGGCGGGGCGGCGGAGCA
  GGAGGAGCTGCACTACATCCCCATCCGCGTTCTGGGCCGCGGTGCCTTTGGGGAGGCCACGCTGTACCGC
  CGCACCGAGGACGACTCACTGGTGGTGTGGAAGGAAGTTGACTTGACCCGGCTATCTGAGAAGGAACGTC
  GAGATGCCCTGAATGAGATTGTCATCCTGGCACTGCTACAGCACGACAACATCATTGCCTACTACAACCA
  CTTCATGGACAACACCACCCTGCTGATTGAGCTGGAGTACTGTAACGGAGGGAACCTCTATGACAAAATC
  CTTCGTCAGAAGGACAAGTTGTTCGAAGAAGAGATGGTGGTGTGGTACCTTTTTCAGATTGTTTCAGCAG
  TGAGCTGTATTCATAAAGCTGGAATCCTCCATAGAGATATAAAGACATTAAATATTTTTCTGACCAAGGC
  AAATCTGATAAAGCTTGGAGACTATGGCCTAGCAAAGAAACTTAATTCTGAGTACTCTATGGCCGAGACA
  CTTGTGGGCACTCCATATTACATGTCTCCAGAGCTCTGCCAAGGAGTAAAATACAATTTCAAGTCTGATA
  TATGGGCAGTTGGCTGTGTCATTTTTGAACTGCTTACTCTGAAAAGAACATTTGATGCTACAAACCCACT
  CAACCTATGTGTGAAGATCGTGCAGGGAATCCGGGCCATGGAAGTTGACTCGAGTCAGTATTCTTTGGAA
  CTGATCCAGTTGGTACACGCATGCCTCGACCAGGATCCTGAGCAGAGGCCCGCTGCAGATGCACTCCTGG
  ATCTCCCCCTTCTCAGGACCCGCAGGAGAGAGATGGAGGAAAAAGTCACTCTGCTCAATGCACCTACAAA
  GAGACCAAGGTCAAGCACTGTGACGGAAGCACCCATTGCTGTGGTAACATCACGAACCAGTGAAGTCTAT
  GTTTGGGGTGGTGGAAAATCCACACCTCAGAAATTGGACGTCATCAAGAGTGGCTGTAGTGCTCGACAGG
  TGTGTGCGGGGAACACCCACTTTGCTGTGGTCACCGTGGAGAAGGAACTATATACCTGGGTGAACATGCA
  AGGGGGCACTAAACTTCACGGTCAGCTAGGACATGGAGACAAAGCTTCCTACCGACAGCCAAAGCATGTA
  GAAAAGTTACAAGGGAAGGCTATCCACCAGGTGTCGTGTGGGGATGATTTCACTGTCTGTGTCACAGATG
  AGGGTCAGCTCTACGCCTTTGGATCAGATTATTATGGCTGCATGGGAGTGGACAAGGTTTCTGGCCCTGA
  AGTGCTAGAACCCATGCAGCTGAACTTCTTCCTCAGCAATCCTGTGGAGCAGGTCTCCTGTGGGGATAAT
  CATGTGGTAGTTCTGACACGAAACAAAGAAGTCTACTCTTGGGGCTGTGGTGAATATGGACGGCTGGGTT
  TGGATTCAGAAGAGGATTACTATACACCACAGAGGGTGGATGTTCCAAAGGCCTTAATCATTGTTGCAGT
  TCAATGTGGCTGTGATGGGACCTTTCTGCTCACTCAGTCAGGCAAAGTGCTGGCCTGTGGACTCAATGAG
  TTCAACAAGCTGGGGCTGAATCAGTGCATGTCTGGAATCATCAACCATGAAGCATACCATGAAGTTCCCT
  ACACAACCTCCTTTACCTTGGCCAAACAGTTGTCCTTTTACAAAATCCGTACCATTGCCCCAGGCAAGAC
  TCACACAGCTGCTATTGATGAACGAGGCCGGTTGCTGACTTTTGGCTGTAATAAGTGTGGGCAGCTGGGT
  GTTGGGAATTATAAGAAACGCCTGGGAATCAACCTGTTGGGGGGACCCCTTGGCGGGAAGCAAGTGATTA
  GGGTCTCGTGTGGCGATGAGTTCACCATTGCTGCCACCGACGATAATCACATCTTTGCCTGGGGTAATGG
  TGGTAATGGCCGCCTGGCAATGACTCCCACAGAGAGACCACATGGCTCTGATATCTGTACCTCATGGCCT
  CGGCCTATTTTTGGATCCCTGCATCATGTTCCAGACCTTTCTTGCCGTGGCTGGCACACCATTCTTATTG
  TTGAGAAAGTGTTGAATTCCAAGACCATCCGTTCCAATAGCAGTGGCCTGTCCATCGGAACTGTGGTTCA
  GAGCTCCAGCCCAGGAGGTGGCATTGGTGGTGGTGGTGGTGGTGGCGGCGGCGGCGGGGGTGAAGAGGAA
  GACAGTCAACAGGAATCTGAAACTCCAGATCCAAGCGGAGGCTTCCGAGGAACAATGGAAGCAGACCGTG
  GGATGGAAGGCTTAATCAGTCCCACGGAGGCCGTGGGAAACAGCTGTGGGGCTAGCAGCTCCTGTCCTGG
  CTGGCTTCGGAAGGAGCTGGAAAACGCAGAGTTCATTCCCATGCCTGACAGCCCCGCTCCCTTAAGTGCG
  GCGTTTTCACAGTCTGAGAAGGACACGCTGCCCTATGAAGAGCTGCAGGGACTCAAGGTCGCATCTGAAG
  TTCCTCCGGAGCCCCAGCGTGCAGCAGGAGCCTGGCCCCCTCGGCTGGATCCTGCAGTGCCGTGTGTTGG
  GAAAGCCCTGACCTCTGCTGCTTGTGCGTGCAGTGCTCTGCAGGTGGAGGTTGACAGGTTGCAGGCTCTG
  GTGTTAAAGTGTCTGGAAGAACAGCAGAAGCTCCAGCAAGAAAACCTCCAGATGTTTACCCAACTACAAA
  AGCTCAACAAGAAATTGGAAGGAGGCCAGCAGGTGGGGATGCACTCCAGAGGAACCCAGACAGCAAAGGA
  AGAAATGGAAATGGATCCAAAGCCTGACTTAGACTCGGAGTCCTGGTGCCTCCTCGGAACAGACTCTTGT
  CGACCCAGCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216306 representing NM_145138
  Red=Cloning site Green=Tags(s)
  
  MSVLGEYERHCDSINSDFGSESGGGGDSGPGPSAVPGPRAGGGAAEQEELHYIPIRVLGRGAFGEATLYR
  RTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKI
  LRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAET
  LVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLE
  LIQLVHACLDQDPEQRPAADALLDLPLLRTRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVY
  VWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHV
  EKLQGKAIHQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVSGPEVLEPMQLNFFLSNPVEQVSCGDN
  HVVVLTRNKEVYSWGCGEYGRLGLDSEEDYYTPQRVDVPKALIIVAVQCGCDGTFLLTQSGKVLACGLNE
  FNKLGLNQCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLG
  VGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWP
  RPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGLSIGTVVQSSSPGGGIGGGGGGGGGGGGEEE
  DSQQESETPDPSGGFRGTMEADRGMEGLISPTEAVGNSCGASSSCPGWLRKELENAEFIPMPDSPAPLSA
  AFSQSEKDTLPYEELQGLKVASEVPPEPQRAAGAWPPRLDPAVPCVGKALTSAACACSALQVEVDRLQAL
  VLKCLEEQQKLQQENLQMFTQLQKLNKKLEGGQQVGMHSRGTQTAKEEMEMDPKPDLDSESWCLLGTDSC
  RPSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145138
• ORF Size: 2952 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145138.2, NP_660120.2
• RefSeq Size: 5403 bp
• RefSeq ORF: 2955 bp
• Locus ID: 217718
• UniProt ID: Q8K1R7
• Cytogenetics: 12 D1
• Gene Summary: Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively (By similarity).[UniProtKB/Swiss-Prot Function]