Aars (NM_146217) Mouse Untagged Clone

CAT#: MC222885

Aars (untagged) - Mouse alanyl-tRNA synthetase (Aars), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aars
• Synonyms: AI316495; C76919; sti
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222885 representing NM_146217
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCACTTTAACAGCACGAGAGATCCGAGAGCGGTTTATAAATTTCTTCAGACGAAATGAGCACA
  CCTATGTCCACTCTTCCGCCACCATCCCTCTGGATGACCCCACTCTGCTCTTCGCCAACGCTGGCATGAA
  CCAGTTCAAACCCATCTTCCTAAACACAATCGACCCATCTCACCCTATGGCGAAGCTGAGCAGAGCTGCC
  AATACCCAGAAATGCATCCGAGCTGGGGGCAAGCACAACGACCTGGATGACGTGGGCAAGGATGTCTACC
  ACCACACTTTCTTTGAGATGCTGGGCTCCTGGTCTTTTGGGGACTATTTCAAGGAATTGGCATGTAAGAT
  GGCCCTGGAACTTCTTACCCAGGAGTTTGGTATTCCAGTTGAAAGACTCTATGTCACTTACTTTGGTGGT
  GATGAGGCGGCCGGCCTAGAGCCAGATCTGGAGTGCAGACAGATTTGGCAAAATTTAGGACTGGATGAAG
  CCAGAATCCTCCCTGGCAACATGAAGGACAACTTCTGGGAGATGGGTGACACAGGCCCCTGTGGTCCCTG
  CAGCGAGATCCACTATGACCGCATTGGTGGCAGGGACGCCGCACACCTTGTCAACCAGGATGACCCCAAT
  GTGCTGGAGATCTGGAACCTCGTGTTCATCCAGTATAACAGGGAGTCGGATGGTGTTCTGAAACCTCTTC
  CCAAGAAAAGCATCGACACAGGGATGGGCCTAGAGAGGCTGGTGTCTGTGCTGCAGAACAAGATGTCCAA
  CTACGACACCGACCTTTTCATGCCTTACTTCGAAGCCATTCAGAAGGGCACCGGCGCCCGGCCGTATACT
  GGGAAGGTTGGTGCTGAGGATGCAGACGGAATCGACATGGCCTACCGGGTTCTGGCTGACCACGCCCGGA
  CCATCACTGTGGCGCTGGCTGATGGCGGCCGGCCTGACAACACAGGACGGGGGTACGTGCTGAGACGGAT
  CCTTCGCCGAGCTGTTCGGTATTCCCATGAGAAACTGAATGCCAGCAGGGGTTTCTTCGCTACATTGGTT
  GATGTTGTCGTTCAATCCCTGGGAGACGCATTTCCTGAGCTGAAGAAGGACCCAGAGATGGTGAAGGACA
  TCATTAATGAAGAAGAGGTGCAGTTTCTCAAGACTCTCAGCCGAGGGCGGCGCATCCTGGACCGAAAAAT
  TCAGAGCTTAGGAGACTGCAAAACCATCCCAGGTGACACCGCTTGGCTCCTCTATGATACTTATGGATTC
  CCAGTGGACCTCACTGGATTGATTGCTGAAGAGAAGGGCCTGGTGGTAGATATGAATGGCTTTGAGGAAG
  AGAGGAGACTGGCCCAGCTGAAGTCACAGGGCAAGGGAGCAGGGGACGAAGACCTCATCATGCTGGACAT
  TTACGCTATTGAAGAGCTCCGGGCAAAGGGTTTGGAGGCCACAGATGATTCTCCAAAGTATAACTACCAA
  TCAGACTCCAGTGGTAGCTACGTGTTTGAGTGTACAGTGGCTACCGTGCTTGCTCTGCGTCGGGAAAAGA
  TGTTTGTGGACGAGGTGGTCACTGGCCAGGAGTGTGGAGTGGTGCTGGACAAGACCTGTTTCTATGCGGA
  GCAAGGAGGGCAGATCTACGATGAAGGCTACTTGGTGAAGGTCGACGACAGCAGTGAGGACAAAACCGAG
  TTTACAGTGAAGAACGCTCAGGTCCGAGGCGGGTACGTGCTGCACATAGGAACGATATATGGTAACCTGA
  AAGTGGGGGATCAAGTCCGGCTGTTTATTGATGAGCCCCGGCGGAGACCCGTCATGAGCAACCACACAGC
  TACACACATCCTCAACTTCGCCTTGCGCTCGGTGCTTGGGGAAGCGGACCAGAAAGGTTCACTGGTTGCT
  CCTGACCGCCTTCGGTTTGACTTCACTGCCAAGGGAGCCATGTCCACCCAGCAAATCAAGAAGGCTGAAG
  AGATTGTCAATGGCATGATTGAGGCAGCTAAGCCTGTCTACACCCAGGATTGTCCCCTGGCAGCAGCAAA
  AGCCATCCAGGGCCTTCGAGCTGTGTTTGATGAGACCTATCCTGACCCCGTTCGAGTCGTCTCCATTGGG
  GTCCCCGTGTCAGAGCTGCTGGATGACCCGTGTGGGCCTGCTGGCTCCCTTACTTCTGTTGAGTTCTGTG
  GAGGAACACATCTACGGAATTCTAGCCACGCAGGCGCTTTTGTGATAGTGACAGAAGAAGCTATTGCCAA
  GGGTATCCGGAGGATTGTTGCTGTCACAGGTGCTGAAGCCCAGAAGGCTCTCAGGAAGTCAGAAACCTTG
  AAGAAATCTCTCTCTGCCATGGAGGCCAAAGTGAAGGCCCAGACTGCACCCAACAAGGACGTGCAGAGGG
  AAATTGCTGACCTCGGTGAGGCCCTGGCCACTGCAGTCATCCCCCAGTGGCAGAAAGATGAACAACGAGA
  GACTCTGAAGTCCCTGAAGAAAGTCATGGATGACCTAGACCGGGCCAGCAAAGCCGATGTGCAGAAGCGA
  GTGTTAGAGAAGACAAAGCAGCTGATTGACAGCAACCCCAACCAGCCGCTGGTCATCCTGGAGATGGAGA
  GTGGAGCATCGGCCAAGGCCCTGAATGAAGCGTTGAAGCTTTTCAAGACGCATTCTCCTCAGACATCTGC
  CATGCTGTTCACGGTGGACAATGAGGCTGGCAAGATCACGTGCTTGTGCCAGGTTCCCCAGAATGCAGCC
  AACCGGGGACTGAAAGCCAGCGAGTGGGTCCAGCAGGTTTCAGGCCTGATGGATGGTAAAGGCGGTGGCA
  AGGACATGTCCGCCCAGGCCACAGGCAAGAACGTGGGCTGCCTTCAGGAAGCACTGCAGCTGGCTACCTC
  CTTTGCCCAGCTCCGTCTGGGAGATGTGAAGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146217
• Insert Size: 2907 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146217.4, NP_666329.2
• RefSeq Size: 3728 bp
• RefSeq ORF: 2907 bp
• Locus ID: 234734
• UniProt ID: Q8BGQ7
• Cytogenetics: 8 E1
• Gene Summary: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala) (PubMed:16906134, PubMed:20010690, PubMed:25422440, PubMed:27622773). Also edits incorrectly charged tRNA(Ala) via its editing domain (PubMed:16906134, PubMed:20010690, PubMed:25422440, PubMed:29769718).[UniProtKB/Swiss-Prot Function]