Grik1 (NM_146072) Mouse Untagged Clone

CAT#: MC222680

Grik1 (untagged) - Mouse glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grik1
• Synonyms: A830007B11Rik; D16Ium2; D16Ium24; D16Ium24e; Glu; GluK; GluK1; GluK5; Glur; Glur-5; Glur5; Glurbe; Glurbeta1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222680 representing NM_146072
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCGGCACAGTCCTTATCCAACCCGGGCTCTGGACCAGGGACACCAGCTGGACACTCCTCTATT
  TCCTGTGCTACATCCTTCCTCAGACCTCCCCTCAAGTGCTCAGGATCGGAGGGATTTTTGAAACTGTGGA
  AAACGAACCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGTATTAACCGAAACCGAACC
  TTGATGCCCAATACCACATTAACCTATGACATCCAGAGAATTAATCTTTTTGATAGTTTTGAAGCCTCCC
  GAAGAGCATGCGACCAACTGGCTCTTGGGGTGGCCGCCCTCTTCGGTCCTTCCCACAGCTCCTCCGTCAG
  TGCTGTACAGTCTATTTGCAATGCTCTGGAAGTTCCACACATTCAGACTCGCTGGAAACACCCTTCTGTG
  GACAACAGAGACTTATTTTACATCAACCTCTACCCAGATTATGCAGCTATCAGCAGGGCAGTCCTGGATC
  TGGTCCTCTATTACAACTGGAAAACAGTGACGGTGGTGTACGAAGACAGCACAGGTCTAATTCGTCTGCA
  AGAGCTCATCAAAGCTCCCTCCAGATACAACATCAAAATCAAAATCCGCCAGCTTCCCTCTGGCAATAAG
  GATGCCAAACCTCTGCTCAAGGAGATGAAGAAAGGCAAGGAGTTCTATGTGATATTTGATTGTTCGCACG
  AGACGGCTGCTGAAATTCTTAAGCAGATTTTGTTCATGGGCATGATGACTGAATACTATCACTACTTCTT
  CACAACCCTGGACTTGTTTGCTTTGGATCTGGAACTCTACAGGTACAGTGGTGTAAATATGACTGGATTT
  CGGTTGCTGAATATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGATTGCAGG
  CCCCACCCAGACCTGAGACTGGTCTCCTGGACGGCGTGATGACAACTGAAGCAGCTCTGATGTACGATGC
  TGTGTACATGGTAGCCATCGCCTCTCACCGTGCCTCTCAGCTGACCGTCAGTTCCCTGCAGTGCCATCGA
  CATAAGCCATGGCGCCTAGGACCCAGATTTATGAACCTCATCAAAGAGGCGCGGTGGGATGGCTTGACGG
  GGCGGATCACCTTCAATAAGACGGATGGCTTGAGAAAGGATTTTGACCTGGACATTATCAGTCTCAAAGA
  GGAAGGAACTGAAAAGATTGGGATTTGGAACTCCAACAGTGGGCTGAACATGACGGATGGCAACAGAGAC
  AGGTCCAACAATATCACAGATTCGCTGGCTAACCGAACGCTCATTGTCACCACTATTCTGGAAGAGCCCT
  ACGTGATGTACAGGAAATCCGATAAACCACTGTACGGAAATGACAGATTTGAAGGATATTGCCTGGATCT
  GCTGAAAGAACTGTCAAATATCCTAGGTTTCCTTTATGATGTTAAACTGGTTCCTGACGGCAAATATGGA
  GCCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAGGAACTCATCGACCACAGAGCTGACCTAGCAG
  TGGCCCCTCTCACCATCACGTATGTACGGGAGAAAGTCATTGACTTCTCCAAGCCTTTCATGACTCTGGG
  CATTAGCATCCTTTACCGGAAGCCCAATGGAACCAACCCCGGCGTCTTCTCCTTCCTCAACCCCCTGTCT
  CCAGACATTTGGATGTATGTGCTCCTCGCTTGCCTAGGAGTCAGTTGTGTGCTTTTTGTGATTGCAAGGT
  TCACACCCTACGAGTGGTATAACCCCCACCCGTGCAACCCTGACTCAGACGTGGTGGAAAACAATTTCAC
  TTTGCTAAATAGTTTCTGGTTTGGCGTTGGAGCTCTCATGCGGCAAGGATCGGAGCTGATGCCCAAGGCT
  CTATCGACCAGAATAGTTGGAGGAATATGGTGGTTTTTCACCCTAATCATCATCTCATCCTACACTGCCA
  ACCTGGCTGCCTTCTTGACAGTAGAAAGGATGGAATCCCCCATCGATTCCGCAGACGACCTGGCCAAACA
  AACCAAGATAGAATACGGGGCAGTCAGAGATGGCTCGACAATGACCTTCTTCAAGAAATCAAAAATCTCC
  ACGTATGAGAAAATGTGGGCTTTCATGAGCAGTAGACAGCAGAGCGCCCTGGTTAAAAACAGCGATGAGG
  GGATCCAAAGGGTGCTTACCACCGACTACGCCCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACACA
  GAGGAACTGCAACCTCACTCAGATCGGGGGCCTCATAGACTCCAAAGGCTATGGAGTGGGGACACCTATC
  GGCTCCCCTTACCGGGATAAAATTACAATTGCTATTCTTCAACTACAAGAAGAAGGGAAGCTTCATATGA
  TGAAAGAGAAATGGTGGAGGGGAAATGGCTGCCCTGAAGAAGACAGTAAAGAAGCCAGTGCTCTAGGAGT
  GGAAAATATCGGGGGTATCTTCATTGTTCTGGCTGCAGGACTCGTCCTTTCTGTGTTTGTAGCCATTGGA
  GAATTCATATACAAATCACGGAAGAACAATGACATTGAGCAGAAAGGAAAGTCATCAAGACTTAGATTTT
  ATTTTAGGAACAAAGTAAGGTTTCATGGGAGCAAAACAGAAAGCCTTGGTGTAGAGAAGTGTCTCTCTTT
  CAATGCCATCATGGAAGAGCTGGGAATCTCACTCAAGAATCAGAAAAAATTAAAGAAAAAGTCAAGAACT
  AAGGGCAAATCTTCTTTCACAAGTATCCTTACTTGTCATCAGAGACGAACTCAGAGAAAAGAGACTGTGG
  CGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146072
• Insert Size: 2805 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146072.4, NP_666184.2
• RefSeq Size: 3660 bp
• RefSeq ORF: 2805 bp
• Locus ID: 14805
• Cytogenetics: 16 50.23 cM
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (a). RNA editing (CAG->CGG) changes Gln621Arg.