Zfyve28 (NM_001015039) Mouse Untagged Clone

CAT#: MC222558

Zfyve28 (untagged) - Mouse zinc finger, FYVE domain containing 28 (Zfyve28), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfyve28
• Synonyms: 9630058O20Rik; Gm146; mKIAA1643
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222558 representing NM_001015039
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAACAGATTCCGAAAGTGGCTGTACAAACCCAAGAGGTCAGACCCACAACTGCTGGCCCAGTTCT
  ACTATGCTGATGAGGAGCTGAACCAGGTGGCTGCAGAGCTGGACAGCCTGGATGGGCGGAAGGAGCCCCA
  GAGGTGCACACTGCTTGTCAGCCAGTTCCGCTCCTGTCAGGACAATGTATTAAACATCATTAATCAGATC
  ATGGAGGAGTGTATCCCTCAGGACCGTGCCCCTCGGGATTTCTGCGTCAAGTTTCCAGAGGAGATCCGGC
  ATGACAACTTGGCTGGCCAGCTGTGGTTCGGGGCTGAGTGCCTGGCAGCTGGCTCCATCATCATGAATCG
  GGAGCTGGAGAGCATGGCCATGCGGCCACTGGCCAAGGAGCTGACACGCAGCCTGGAGGACGTGCGGGGC
  ACTCTCCGTGACCAGGCACTCCGAGACCTCAACACTTACACAGAGAAGATGCGGGAGGCACTGAGGCGCT
  TTGATGTCCTGTTTGCTGAGTTTGAACTGAGCTATGTCTCAGCCATGGTGCCAGTGAAGTCCCCCAGGGA
  GTACTATGTGCAGCAGGAGGTCATCGTGCTGTTCTGTGAGACAGTGGAGAGAGCCTTGGATTTTGGGTAT
  CTGACCCAGGACATGATCGACGACTACGAACCCGCCCTCATGTTCACCATTCCTCGGCTGGCCATTGTGT
  GTGGCCTTGTGGTCTATGCAGATGGACCCCTGAACTTGGACCGGAAGGTGGAGGACATGTCTGAGCTGTT
  CCGGCCCTTCCACACGTTGCTGCGGAAAATAAGGGATCTGCTGCAGGCGCTGACGGAGGAGGAGCTGCAC
  ACACTGGAGCGGAGCCTCTGTGTTTCTCAGGATGTGGAGCTCCCCATCCGGGCAGACACCCAGGCACCCA
  GTGCCCTGGCACCCACCTTTTCTGCATCTCTTCCTCCCGAGGAGACACTTTCAGCCAGTGCCAACAACCC
  AGAGGCAGAACTGGCCTGCTCAATGCAGTATGATGATCAGGAGTTGGAGGAACTCAGCCGCATGGTCCAC
  AGGGCTGGGGATGAGATGTCTTCTCTGCTTTCACCACCCAGTGCCTGCCAGTCCCCGGCACATAGGCCAG
  GCTCAGAGGCTAGCCCTCGAGGGGAGGCCTCTCCAGCCAGAGCCCGGCTGAAATCAGGCAGTGATGAGGA
  GGAAAGGGTGTTCTTCATGGATGATGTGGAGGTGACAGAGTCTCCTGCCAGGCCTGAATCCCCAGGGAAC
  ACATTTGAGTTGACGCAGGGTAATGCCCAGCAGAGAGGACAAGATGGCCAGAGTGGAGAGGTGGGGGTCG
  AGGCACCTGCCCTGGTAAAGGAGGAAGACTGGAGCAACAATAATGTTGAGGGTGACAAGATAAAGCTGGC
  CAGCCTCATGGGCTCCACTTCCTGCAGCTGCCTAGACTCACAGCTCTACCTGGACGGCTGGGAGGTGAGT
  GCAGAGGATGCTGAGACTGCAGAGATGATTGCACACCGGACCGGGGGAATGAAACTGTCAGCCACAGTCA
  TCTTCAACCCCAAGTCTCCCACTTCCCAGGACTCAGCTGTGGCAGCCCAAGAAGCCCCAGGCCATGGTAC
  TTCCCCTTTGGAGCCCAGGGCAGAGGGGACAGGGGACAATTCACACAAACTCAGCACTACAGCCACCAAC
  TGCCTCCTCCATTCCTGTGTGTGCTGTGGGAGCTGTGGGGACAGCAGGGACGATGCTGTAGAGCGCCTGC
  GGGAGAAGTGTGGCCCAGGAAGTGTCATCAGTGCCTCTAACCCTTCTGTAAGCTTAGCCAAGGGTGGTGA
  CAAAGAGCCCGAGAGAATAGATGAGGCCCAGCCTTCTGATGTCACCCTGCCTGCAGAAGATGCCTCCAAC
  AGGCAGGAGCCCAAAGCCCCTGCTTCCAGCAAGTGCCTGGCACACACCTCAGGGCCTCAGGTGGACACAG
  CGAGCAGGTTGCAAGGAGAGGGTGAGGTCAAAGGCCAGCCAGAGCCAGAGGCCAGAAAGCAGGACCCTGA
  GAAGTCCCCTGTGGTCAGCGGGGACAGTCCAAGAGGGGATGTGGCCCAGACAGAACACCAGCACCTGTTG
  GGCTCCAGCTCCACGGTTGGTTCCTGCTCCTTAGACAACACCAGGTTGGATGTGGCCACTGCTGCCATGC
  CTGTCACCCCCATGGCCACCAGAGAGAAGATCCGGTCCAGGTTCCATGGCAGCCATGACCTGATCCACCG
  CCTGTTTGTCTGCATTTCAGGTGTAGCAGACCAGCTACAGACGAACTATGCTAGCGACCTGAGAAGTATT
  CTCAAAACACTGTTTGAGGTCATGGCCACCAAGCCAGAAACAGATGACAAAGAGAAGCTAAAGAAGGTAA
  CTCAGACCCTGCGGAGTGCAGCCTTGGAGGACTGTGCACTGTGCCAGGAGACTGTGTCATCCTCAGAACT
  GGCAGCCAAGACCCGAGATGGGGACTTTGAAGACCCTCCTGAGTGGGTGCCAGATGAGGCCTGTGGCTTC
  TGCACCTCCTGTAAAGCTCCCTTCACCGTCATCCGCAGGAAGCACCACTGTCGCAGCTGTGGTAAGATCT
  TCTGTTCCCGCTGCTCATCCCACTCGGCGCCGCTTCCGCGCTACGGACAGGTGAAGCCCGTCCGTGTGTG
  TACACACTGCTACATGTTCCACGTCACACCCTTCTACAGTGACAAGACGGGCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001015039
• Insert Size: 2718 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001015039.1, NP_001015039.1
• RefSeq Size: 3980 bp
• RefSeq ORF: 2718 bp
• Locus ID: 231125
• UniProt ID: Q6ZPK7
• Cytogenetics: 5 B2
• Gene Summary: Negative regulator of epidermal growth factor receptor (EGFR) signaling. Acts by promoting EGFR degradation in endosomes when not monoubiquitinated (By similarity).[UniProtKB/Swiss-Prot Function]