March6 (NM_172606) Mouse Untagged Clone

CAT#: MC222553

41339 (untagged) - Mouse membrane-associated ring finger (C3HC4) 6 (March6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: March6
• Synonyms: 3830408G03; F830029L24Rik; mKIAA0597
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222553 representing NM_172606
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACCGCCGAGGAAGATATATGTAGAGTGTGTCGGTCAGAAGGAACACCTGAGAAACCTCTTTATC
  ATCCTTGTGTATGTACTGGCAGTATTAAATTTATTCATCAAGAATGCTTAGTTCAGTGGTTGAAACATAG
  TCGGAAAGAATACTGTGAATTATGCAAGCACAGATTTGCTTTCACACCAATTTATTCTCCAGATATGCCT
  TCACGGCTACCAATCCAAGACATATTTGCTGGACTGGTTACAAGTATTGGCACTGCAATACGATACTGGT
  TTCATTACACACTTGTGGCCTTTGCATGGCTGGGAGTTGTTCCTCTTACAGCATGCCGCATCTACAAGTG
  CTTGTTTACTGGCTCCGTGAGCTCACTACTGACACTGCCACTAGACATGCTGTCAACGGAAAATTTGTTG
  GCGGATTGCTTGCAGGGCTGTTTTGTGGTGACGTGCACACTTTGTGCGTTCATCAGCCTGGTGTGGTTAC
  GAGAGCAGATAGTCCACGGGGGAGCCCCAATTTGGCTGGAGCATGCTGCTCCACCCTTCAATGCTGCTGG
  ACACCACCAAAATGAGGCTCCTGTAGGAGGAAATGGTGCAGAAAACCCTGCTGCTGATCAGCCAGCTAAC
  CCAGCAGGTGAGAATGCAGTTCTGGGTGAAAACCCTGATGCCCAGGACGGTCAAGCAGAAGAGGAAGAGG
  AGGACAATGAGGAGGAAGATGATGCGGGTGTAGAAGATGCTGCTGATGCCAACAATGGAGCCCAAGACGA
  CATGAACTGGAATGCTTTAGAGTGGGACCGGGCTGCAGAAGAGCTTACTTGGGAAAGAATGCTTGGACTT
  GATGGATCACTAGTTTTCCTGGAACACGTGTTCTGGGTGGTGTCTTTAAATACATTGTTCATTCTTGTTT
  TTGCATTTTGTCCTTACCACATCGGCCATTTCTCCCTTGTTGGCTTGGGGTTTGAGGAGCATGTCCAAGC
  ATCTCATTTTGAAGGTCTAATCACAACGATTGTTGGATATATACTTTTAGCAATAACACTGATAATTTGT
  CATGCATTGGCAACTCTTGTTAAATTTCATAGGTCTCGTCGCTTGCTGGGCGTCTGCTATATTGTGGTTA
  AGGTCTCTTTGTTAGTTGTGGTAGAAATCGGAGTGTTCCCTCTCATTTGTGGCTGGTGGCTGGATATCTG
  TTCTCTGGAAATGTTTGATGCTACTCTGAAAGATCGAGAGCTGAGCTTTCAGTCAGCTCCAGGGACCACG
  ATGTTTCTGCACTGGTTGGTGGGAATGGTGTATGTGTTCTACTTTGCCTCCTTCATCCTGTTACTGAGAG
  AGGTACTTCGACCTGGTGTCTTATGGTTTCTGAGGAATTTGAACGATCCAGATTTTAATCCAGTACAGGA
  AATGATTCACTTGCCCATTTATAGACATCTCCGAAGATTTATTTTGTCAGTGATTGTCTTTGGCTCCATT
  GTCCTTCTGATGCTCTGGCTTCCTATACGTATAATTAAGAGTCTCTTGCCTAATTTTCTTCCTTACAATG
  TCATGCTCTACAGTGATGCTCCGGTGAGTGAGCTGTCCCTTGAGCTGCTCCTACTGCAGGTTGTCTTGCC
  AGCATTGCTGGAACAGGGACACACGAGGCAGTGGCTGAAGGGGCTTGTGCGTGCATGGACTGTTACTGCT
  GGATACTTGCTGGACCTTCATTCCTACTTACTGGGAGATCAGGAAGAAAACGAGAACAGTGCAAATCAGC
  AGGTCAACAATAACCAGCCTGCACGAAACAATAATGCTGTTCCTGCCGGGGAAGGTCTGCATGCAGCCCA
  CCAAGCCATACTCCAGCAGGGAGGACCTGTTGGCTTTCAGCCTTACCGCCGGCCTTTAAATTTCCCACTC
  AGGATATTTCTGCTGATTGTCTTCATGTGCATAACATTGCTAATTGCCAGCCTCATTTGCCTTACTTTAC
  CAGTATTTGCTGGCCGTTGGTTAATGTCATTTTGGACGGGGACTGCCAAAATCCATGAGCTGTACACAGC
  TGCTTGTGGTCTCTATGTGTGTTGGCTAACCATAAGGGCTGTGACGGTGCTGGTGGCCTGGATGCCCCAG
  GGACGAAGAGTGATTTTCCAGAAGGTTAAAGAGTGGTCCCTCATGATAATGAAGACGTTGATAGTTGCTG
  TGCTGCTGGCTGGGGTCGTCCCACTGCTCCTCGGGCTCCTGTTTGAGCTGGTTATTGTTGCTCCTCTGAG
  GGTCCCTCTTGATCAGACTCCCCTTTTCTACCCTTGGCAGGACTGGGCGCTGGGAGTGCTGCATGCCAAA
  ATTATCGCAGCTATAACTCTTATGGGTCCTCAGTGGTGGTTGAAAACTGTCATTGAACAGGTTTACGCAA
  ATGGCATTCGTAACATCGACCTTCACTATATCATCCGTAAGCTGGCAGCCCCGGTGATCTCTGTGCTCTT
  ACTTTCCCTGTGTGTACCGTATGTCATAGCTTCTGGCGCGGTTCCTCTACTAGGTGTTACTGCAGAGATG
  CAGAACTTAGTTCACCGGCGGATTTACCCATTTTTATTGATGGTCGTTGTGCTGATGGGAATCCTGTCCT
  TCCAGGTCCGCCAGTTTAAGCGCCTTTATGAACACATTAAAAATGACAAGTACCTTGTGGGGCAGCGCCT
  GGTGAACTATGAGCGCAAATCCGGCAAGCAAGGCCCGTCTACACCGCCTCCGGTGTCGTCCCAAGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172606
• Insert Size: 2730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172606.2, NP_766194.2
• RefSeq Size: 6236 bp
• RefSeq ORF: 2730 bp
• Locus ID: 223455
• UniProt ID: Q6ZQ89
• Cytogenetics: 15 B2
• Gene Summary: E3 ubiquitin-protein ligase that promotes 'Lys-48'-linked ubiquitination of target proteins, leading to their proteasomal degradation. Promotes ubiquitination of DIO2, leading to its degradation. Promotes ubiquitination of SQLE, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1.[UniProtKB/Swiss-Prot Function]