Adgrf1 (NM_133776) Mouse Untagged Clone

CAT#: MC222538

Gpr110 (untagged) - Mouse G protein-coupled receptor 110 (Gpr110), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adgrf1
• Synonyms: 5031409J19Rik; Gpr110; KPG_009
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222538 representing NM_133776
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAATTGGACTTCTGTGGCTCGTCCCTCTCTTCACGCTCACTGAGGGCACCGATGGCTTCCTGCAGC
  AGAAAAATGATGGCCGAAGAACAAAAGAAATTGTCAGTATGGTTGAGGAAAGGCATCCAGTCCACGAGTA
  TGAGGTGCTACTGCAGGTGACATACAGAGATCCAGAGGAGAAGAGAGAACTGAAGAGATTTCTGAAGCTC
  TTGAAATCTCCGTCGCCGAGTTTACGTGGTCCCAGCAAGATCATCAGAGTGAAGGCCACCACATACTGCA
  GAAGCCGAAAAGGGTTCCTGGAGTGTGCCTGCGAAGACAGCTATACGTGGTTTCCTCCCTCCTGCCTTGA
  CCCCCAGAACTGCTGCCTTCACACAACTGGACCCGTCCCAAGCTGCAATTGCTCTCTCAGAGGCCTCAGG
  CAGAGCATTAATTTCTGTGAGAGAGCAAAAGTTTGGGGCACTTTTGAAATCGATGAAAAATTTCCTGAAG
  ACCTTTGGAATTCATCATCTGATGTATATGCCCACTACACAGTTGGAATTGAAAATCAACTTAAAGAAGC
  GTACAGAAGAGTCCATGGTTTTGAGTCAGTTCGGGTCACCCAATTTCGGAAGGGAAGCATCGTTGTTGGC
  TACGAAGTGACCGGCTCCACTAGCCCTCCAGAACTCCTCTTTGCCATTGAACAGGAGGCCGAAAAGGCAC
  AGGAGGCCCTAAGGAGGCAGTTTCCAGTCAAATATGGCTCTTTTCGAGTATTCGGGAAAGCTCCGTGTAA
  CAGCATCTCCTTTGGATTCGGGTCTGAGAATGATGAATATACTGTGCCCTGCAGCAGTGGCTTCACAGGA
  AGCATGACGGTCAGGTGCCAGTCCTCTGGGTGGCAGATCACCAGGGAGTCCTGTGTGCTTTCTCAGCTGG
  AAGAACTGAAGAAGGAGCTCAGGATGATCGCAGGCAAGATCACAGAGGCGGGTGTGGCATCTTTGGTGCA
  AAACCTTTCCACCATCCTTTTGCAAAGTCCATCGACCACAGTTGGGAACCTGGGCTCTGTGGTGTCACTT
  CTGAGCAACATTTCATCCCTGTCGCTGGCAAACTCTTTAACTGTGTCCAATCTGACACTGATGAACGTCA
  TCAACATAGCTGATCATATCCTGGATTCAGCCTCCATAACGAACTGGACAATTTTATTACAAGATGCAAA
  GGATGCCAGCTCCCAGCTACTAAAGACATTGGAAAGCATCAGTAGTCTCATCCCTTCCATGGCTCTGCCT
  CTTAATTTTTCTGGAAAATTCATTGACTGGAAAGGGACCCCAGTGACCCAAATCCAGAGCACACGGGGCT
  ACAACTATCAGATGGAAATGAGACAAAATGCCTCTCTGCCTATCCGAGGCCACGTGTTTATTGAACCAGA
  TCAATTCCAGAAATCGCATCCAAAGACTATTATCAGCATGGCCTCATTGACCTTTGGGGACATTCTACCC
  ATTACCCAGAGAGGAAATGCATGGGTCAATGGCCCAGTGATATCCACACTTATTCAAAACTATTCCATCA
  GCGAAATTTTCCTGAACTTTTCCAAAATAAAGGGAAACCTGACTCAGCCTCGCTGTGTGTTTTGGGATTT
  TAGCCAGTTGCAATGGAGCAATGCAGGCTGCCAACTGGTTAATGAAACTCTGGACACGGTACTGTGCCGA
  TGTAGTCACCTGACCTCTTTCTCCATGCTCATGTCACCCTTTGTCCCCTCTTCTGTTGTTCCTGTGGTAA
  AATGGATCACGTACATAGGGCTGAGCATCTCCATTGCAAGTCTAATCTTATGCTTGATCATCGAGTCTCT
  GTTTTGGAAGCAGACCAAGAGAAGCCAAACCTCCTACACACGCAACATTTGCCTGGTGAACATCGCCGTG
  TCACTCTTAATTGCTGATGTTTGGTTTATCATTGCTGCCACTGTGGACCCCTCTGTGAGTCCTTCTGGAG
  TCTGTGTGGCTGCGGTGTTCTTTACCCACTTTTTCTACCTTGCCGTATTCTTTTGGATGCTCGTTCTGGG
  CATCCTGCTGGCTTATCGAATTATCCTAGTGTTTCATCACATGGCTCTGACTACCATGATGGCTATTGGC
  TTTTGCCTAGGCTATGGGTGCCCTCTCCTTATATCCATCATCACCCTTGCTGTCACCCAACCTAGCAATA
  GCTATAAAAGGAACGATGTGTGTTGGCTTAACTGGTCTGACAAGAGCAAACCTCTCTTGGCGTTCGTTGT
  TCCCGCGTTGACTATTGTGGCTGTGAACTTGGTTGTGGTGTTGCTGGTCCTCAGGAAGCTTTGGAGGCCA
  GCAGTTGGAGAAAGACTGAATCAGGATGACAAGGCCACTGCCATCCGCATGGGAAAGAGCCTCCTCGTGC
  TGACCCCGTTGCTAGGGCTCACCTGGGGCTTTGGCATAGGAACAATGGCGAACAGCCACAATCTGGCTTG
  GCATGTTCTTTTTGCTTTACTCAATGCTTTCCAGGGATTTTTCATCTTCTGTTTTGGAATTCTCCTGGAT
  ACCAAGCTGCGGCAACTTCTGTCCAACAAGTTGACTACATTAAGTTCATGGAAGCAAACATCAAAACGGA
  ACGCCTCAGATACAGTTACTCAGCCGAAATGCTTAAGGACATTCAACATACTACAGCACAGAGGCATGTA
  TGCACTTTCTCATACTGGAGATTCGTCCAGTGACATCACATTAACCCAGTTTCTATCAACCGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133776
• Insert Size: 2727 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133776.2, NP_598537.2
• RefSeq Size: 3504 bp
• RefSeq ORF: 2727 bp
• Locus ID: 77596
• UniProt ID: Q8VEC3
• Cytogenetics: 17 B3
• Gene Summary: Orphan receptor.[UniProtKB/Swiss-Prot Function]