Whrn (NM_001008793) Mouse Untagged Clone

CAT#: MC222533

Whrn (untagged) - Mouse whirlin (Whrn), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Whrn
• Synonyms: 1110035G07Rik; AW122018; AW742671; C430046P22Rik; Dfnb3; Dfnb31; Ush2; Ush2d; w; wi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222533 representing NM_001008793
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCACAGCTGGACGGCCTGTCGGTGAGCTCGTCCTCCACCGGCTCGCTGGGCTCGGCGGCGGCGG
  CGGCGGGCGGCGGCGGGGGCGCGGGGTTGCGGCTGCTGTCTGCCAACGTGCGCCAGCTGCACCAAGCGCT
  CACGGCGCTGCTGAGCGAGCCCGAGCGGGAGCAGTTCACTCACTGCCTCAACGCGTACCACGCGCGCCGC
  AACGTCTTCGACCTGGTGCGCACCCTGCGCGTCCTGCTGGACAGCCCGGTCAAGCGGCGTCTGCTGCCCA
  TGCTGCGTCTGGTCATCCCGCGCTCCGACCAGCTGCTCTTCGACCAGTACACGGCCGAGGGCCTCTACCT
  GCCAGCCACCACCCCCTACAGGCAGCCCGCCTGGGCCGCTCCCGACGGCGCGGGGCCCGGGGAGGTGCGA
  CTCGTGAGCCTGCGGCGCGCCAAGGCCCACGAGGGCTTGGGCTTCAGCATCCGCGGGGGCTCGGAACACG
  GCGTGGGCATCTACGTGTCTCTAGTGGAGCCGGGCTCCCTGGCAGAGAAGGAAGGGTTGCGGGTCGGGGA
  CCAGATTCTGCGCGTCAACGATAAATCTCTAGCCCGGGTGACCCACGCGGAGGCTGTCAAGGCTCTCAAA
  GGCTCCAAGAAGCTGGTGCTGTCTGTATACTCAGCTGGGCGTATCCCAGGGGGCTATGTGACCAACCACA
  TCTACACCTGGGTGGACCCACAGGGTCGAAGCACATCCCCTCCCTCCAGCCTGCCCCAGCCCCATGGCAG
  CACCCTGAGACAGCGTGAAGATGACCGAAGGAGTACCCTCCACCTCCTGCAGAGTGGAGATGAGAAAAAG
  GTGAACCTGGTGTTGGGGGACGGCCGGTCCTTGGGCCTCACGATCCGAGGTGGAGCAGAGTACGGCCTTG
  GCATTTACATCACTGGTGTGGACCCAGGCTCTGAAGCAGAGAGCAGCGGCCTCAAGGTTGGAGACCAGAT
  TCTGGAGGTGAATGGGCGGAGCTTTCTCAACATCCTGCATGATGAGGCAGTGAAGCTGCTCAAGTCATCC
  CGGCACCTCATCCTGACGGTGAAGGACGTCGGAAGGCTGCCCCACGCACGTACCACCGTGGACCAGACCA
  AGTGGATCGCCAGTTCCCGGATCGGGGAAAGCGTCGCCAACTCAGCAGGGTTTCCAGGGGACCACACAGA
  AGAAGGGACAAGCAAGCCAGGATTTTACAAGGGCCCAGCCGGCTCCCAGGTGACCCTGAGCAGCCTGGGG
  AATCAGACTCGTGCACTGCTGGACGACCAAGCCAGGCACCTGCTGACAGAGCAGGAGCGCGCCACCATGA
  TGTACTACCTGGCCCAGTACCGTGGTGGCACCATCTCTGTGGAGGCCATGGTCATGGCACTGTTTGAGTT
  GCTCAACACACACGCCAAGTTCTCACTCCTGTCTGAGGTGAGAAGCATCATCTCACCCCAAGACCTGGAC
  CGCTTTGACCACCTGGTGCTGAGGCGCGAGATCGAGTCTATGAAGGCGAGGCAGCCCCCCGGCCCCGGGG
  TTGGAGACACGTATTCCATGGTTTCCTACAGCGACACAGGCTCATCCACAGGCAGCCACGGCACCTCTAC
  AACCGTCAGCTCGGCCAGGAATACATTAGACCTGGAAGGAACCGGTGAGACCACCCAGGGCAGCACCAAC
  GCTCTCCCGGATGTGTCTGTGGATGATGTCAAATCCCCCTCAGAAGATCTGCCTGGTATCAAGCCACCTC
  CTCCCCCACCACCCCTGGCTCAAGGTCATGACCGCTTGCTTGGCCAGCCACGGAAGCCAGGGAGGGAGGA
  CCCCGCACCTCTGTCTTCTGCTGCCCACTCAGGCATTGTCTTCTCAGCACCACGGAACCGCAGCCCGCCG
  CCAGGGACTGCTCCCACTCCGGGACCTTCCTCAGCACAGGACTCTCCCTCCTCCCCCATTTATGCCTCCA
  TCTCCCATGCCAACCCCAGTTCCAGAAAGCCGCTGGACACCCACCTGGCCCTGGTTAACCAGCACCCCAT
  CGGCCCCTTCCCTCGAGTCCAGTCCCCACCTCACCTGAAGAGCCCTCCTGCAGAGACCCCAGGAGCTGGG
  GCCTGCCTTCCACCACCATCACCCTCTGAACACCCTGACGCCGTGGGTGCAAACCAGCACTTTGTTCTGG
  TGGAGGTGCACCGTCCGGACAGTGAGCCTGATGTGAATGAAGTGCGGGCTCTGCCCCAGACTCGCACCTC
  CACACTCTCTCAGCTCTCAGACAGTGGGCAGACCCTGAGCGAGGACAGTGGCGTGGATGCCGGGGAGACG
  GAGGCCAGCACCTCAGGCCGAGGCAGACAGACAGCATCCGCTAAGAACAAGAATGGCAAGGAGCAGCCCC
  GGACGGAGAGGACCGCGGAGGGTGCCAACAAACCTCCCGGCCTGCTGGAGCCAACGTCCACCCTGGTCCG
  TGTGAGGAAAAGTGCAGCCACACTGGGCATCGCCATTGAGGGCGGTGCCAACACACGCCAGCCTCTGCCC
  AGGATCGTCACAATTCAGCGAGGAGGTTCTGCCCATAACTGTGGACAGCTCAAGGTGGGCCACGTAATTC
  TGGAAGTGAATGGGCAGACACTTCGGGGTAAGGAGCACAAGGAGGCCGCCCGAATCATCGCTGAGGCCTT
  CAAGACCAAGGAGAGAGACTACATCGACTTTCTGGTCACTGAGTTCAACGTGATGCTCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001008793
• Insert Size: 2721 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008793.2, NP_001008793.1
• RefSeq Size: 4053 bp
• RefSeq ORF: 2721 bp
• Locus ID: 73750
• UniProt ID: Q80VW5
• Cytogenetics: 4 33.97 cM
• Gene Summary: This gene encodes a protein required for elongation and actin polymerization in the hair cell stereocilia. The encoded protein is localized to the cytoplasm and co-localizes with the growing end of actin filaments. Mutations in this gene have been linked to deafness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]
  Transcript Variant: This variant (4) lacks an in-frame exon and uses an alternate in-frame splice site in the coding region, compared to variant 2. The encoded isoform (4) is shorter than isoform 2.