Ern2 (NM_012016) Mouse Untagged Clone

CAT#: MC222523

Ern2 (untagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 2 (Ern2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ern2
• Synonyms: Ern1; Ire1; ire1-beta; Ire1b; mIre1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222523 representing NM_012016
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGACCAGTCCAGAGATTTCAACTGTGGTCTCCTCTCGGGTTCCTGCTGCAGCTTGTGACGTTGC
  TGGGGAAACTCGGTCCACAGGTCCAGTCTGTCAGGCCAGAGAGCCTTCTGTTTGTGTCTACCTTGGACGG
  AAGTCTCCATGCACTGAACAAGCAGACAGGGGACTTGAAGTGGACAGTGAAGGATGACCCCATCATCCAG
  GGCCCAATGTATGTCACAGAAATGGCCTTTCTCTCTGACCCAGCTGACGGCAGCCTGTATGTCTTGGGAA
  CCCAGAAACAACAGGGGCTGATGAAACTGCCATTCACCATCCCAGAGCTGGTTCATGCCTCTCCCTGTCG
  CAGCTCTGATGGTGTCTTCTACACAGGCCGGAAGCAGGATGCCTGGTTTGTAGTGGACCCTGAGTCAGGG
  GAGACTCAGATGACACTGACCACAGAAGGCCTCTCTACTCCTCAACTTTTCATTGGCCGAACACAGTACA
  CAGTCTCCATGCATGACCTACGGACCCCAGCCCTGCGGTGGAACACAACCTATCGCCGCTACTCAGCACC
  CCTCCTGAATGGCTCACCCGGGAAATACATGAGCCACCTGACATCCTGTGGGATGGGCCTGCTTCTCACT
  GTGGATCCAGGAAGTGGAATTGTGCTGTGGACACAGGACCTGGGAGTGCCTGTGACAGGGATTTACACAT
  GGCACCAGGATGGTCTGCACCAGCTGCCCCATCTCACACTGGCTCGGGACACCCTACATTTCCTTGTCCT
  CCGCTGGGGCCACATCCGTCTTCCTGCCTCCAGCTACCAAGACACAGCCACCCAGTTCTCTTCCTTAGAC
  ACCCAGCTTTTGATGACACTGTATGTGGGGAAAGAAGAAGCTGGGTTCTATGTCTCTAAAGCACTGGTCC
  ACGCTGGGGTGGCCCTCGTGCCCCGTGGACTGACCCTGGCACCCATGGACGGTCCCACAACAGATGAGGT
  GACACTTCAAGTCTCGGGGGAGCGAGAGGGCTCACCCAGCACTGCTGTCAGATACCCCTCAGGCAGCGTG
  GCCCTCCCCAGCCAGTGGCTACTCATTGGATACCACGAACCTCCCCCCGTTCTACACACAACCATGCTGC
  GGGTTCATCCCATCCCAGGGAAAGTGTCTGCTGAGACACGAGCCTCAGAGGACCTCCATGCTCCACCTGT
  CTTTTTTGAGCTCCTAAACCTGAGGAGGGAGGATCCAGAACTACATCCTGAAGAGAAAGCTTCAGACTCT
  TATCCAGGGCTGGGATCCCAAGACCTGCTAGCAGCTACCTTCACTGCCATCCTCCTGGGAGCCTGGGTCC
  TCTACCTGATGAGGCAGCAGCAGCAGAGCCCTTCAGCACCTGCTGGCCCTCCTGACCTCTCACAGGATGC
  TCAGGGACAGCTCTCAAGGGACATCTTACAGGACCAGAGGAGATTTCAAAGCCCCTCAGAGCCAGCCCAG
  CCACCTCATGACCCTGAAGCAGGGCAGCCCACCGTGGTGGGGAAGATTTCCTTCAACCCCAAGGATGTGC
  TGGGCCGTGGGGCAGGAGGGACTTTTGTTTTCCGAGGACAGTTTGAGGGGCGGGCAGTAGCTGTGAAGAG
  GCTCCTCCGAGAGTGCTTTGGCTTGGTTCGGCGAGAGGTGCAGCTGCTGCAAGAGTCTGACCGGCACCCC
  AACGTGCTTCGCTACTTCTGCACTGAGCATGGTCCCCAGTTCCACTACATTGCCTTGGAGCTCTGCCAGG
  CCTCCTTGCAAGAGTACGTGGAGAGCCCAGACCTAGATCGCTGGGGCCTGGAGCCCACCACAGTGCTGCA
  GCAGATGATGTCCGGCCTGGCCCATCTACATTCCTTACACATAGTACACCGTGACCTAAAGCCAGCCAAC
  ATTCTCATGGCTGGGCCTGACAGCCAGGGCCAAGGCAGAGTGGTCATCTCTGACTTCGGCCTCTGCAAGA
  AGCTACCTGTTGGCCGTTGTAGCTTCAGTCTCCATTCTGGTATTCCCGGCACAGAAGGCTGGATGGCACC
  AGAGCTCCTGCAGCTCCCCCCAGACAGCCCGACCAGCGCTGTGGATATCTTCTCCGCAGGCTGCGTATTT
  TATTACGTGCTTTCCGGTGGCAGCCACCCCTTTGGAGAGAGTCTCTACCGACAGGCCAACATCCTCTCAG
  GGGATCCCTGTCTGGCTCAGCTGCAGGAAGAGACCCATGACAAGGTTGTGGCATTGGACTTAGTGAGAGC
  CATGCTGAGCTTGCTGCCCCAGGATCGCCCCTCGGCAGGATGGGTACTGGCTCACCCCCTCTTTTGGAGC
  AGAGCTAAGGAGCTCCAGTTCTTCCAGGATGTCAGTGACTGGTTGGAGAAGGAACCAGATCAGGGGCCTC
  TCGTGTCGGCGCTGGAGGCAGGAAGCTACAAGGTGGTCCGGGAAGACTGGCACAAGCACATCTCAGCCCC
  TCTGCAGGCAGATCTGAAAAGGTTCCGCTCATACAAAGGGACATCAGTTCGAGACCTGCTCCGGGCCATG
  AGGAACAAGAAGCACCACTACAGGGAGCTCCCAGCTGAGGTGCGCCAGACACTAGGCCAACTTCCTGCTG
  GCTTCATTCAGTACTTCACACAGCGATTCCCCAGGCTGTTGCTGCACACTCACCGTGCCATGAGGACCTG
  CGCCTCTGAGAGCCTCTTCCTGCCCTACTACCCACCAGCTCTGGAAGCCAGGAGGCCAGATGCCACAAAG
  AGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012016
• Insert Size: 2736 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012016.3, NP_036146.2
• RefSeq Size: 2988 bp
• RefSeq ORF: 2736 bp
• Locus ID: 26918
• UniProt ID: Q9Z2E3
• Cytogenetics: 7 65.53 cM
• Gene Summary: Role in expression of the DDIT3 transcription factor, required for the unfolded-protein response, growth arrest and apoptosis. Has no effect on 28S ribosomal RNA cleavage, unlike the corresponding human protein.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).