Gria3 (NM_016886) Mouse Untagged Clone

CAT#: MC222422

Gria3 (untagged) - Mouse glutamate receptor, ionotropic, AMPA3 (alpha 3) (Gria3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gria3
• Synonyms: 2900064I19Rik; Glu; GluA3; Glur; Glur-3; GluR-C; GluR-K3; Glur3; Gluralpha3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222422 representing NM_016886
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGGGTCATTCTCACGGAGGAT
  TCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAACACGGTACAGGAGCACAGTGCTTTCCGCTT
  TGCTGTGCAGTTATACAACACCAACCAGAACACCACTGAGAAGCCCTTCCATTTGAACTACCACGTAGAC
  CACTTGGATTCCTCCAATAGTTTTTCTGTGACTAATGCTTTCTGCTCCCAGTTCTCCAGAGGGGTGTATG
  CTATCTTTGGATTCTATGACCAGATGTCAATGAACACACTGACCTCCTTCTGTGGGGCCCTGCATACATC
  TTTCGTCACACCTAGCTTTCCCACTGATGCCGATGTGCAGTTTGTCATCCAGATGCGCCCAGCCTTAAAG
  GGTGCCATTCTGAGTCTTCTGGGTTACTACAAGTGGGAGAAGTTTGTGTACCTCTATGACACAGAACGAG
  GGTTTTCCATCCTGCAAGCAATTATGGAAGCAGCAGTGCAAAACAACTGGCAAGTGACAGCAAGGTCTGT
  GGGAAACATAAAGGACATCCAGGAATTCAGACGCATCATTGAAGAAATGGACAGAAGGCAGGAAAAACGA
  TACTTGATTGACTGTGAAGTCGAAAGGATTAACACAATTTTGGAACAGGTTGTGATCCTGGGGAAACATT
  CAAGAGGTTATCACTACATGCTTGCTAACCTGGGTTTTACTGACATTGTACTGGAAAGAGTCATGCATGG
  GGGAGCCAACATTACAGGTTTCCAGATTGTCAACAATGAAAACCCAATGGTCCAGCAATTCATACAGCGC
  TGGGTGAGACTGGATGAAAGGGAATTCCCTGAAGCCAAGAATGCACCACTAAAGTATACATCTGCACTGA
  CACACGACGCAATACTGGTCATAGCAGAAGCCTTCCGATACCTGAGGAGGCAGCGAGTGGATGTATCCCG
  CAGAGGCAGTGCTGGAGACTGCTTAGCAAATCCTGCTGTGCCCTGGAGTCAAGGAATTGATATTGAGAGA
  GCTCTGAAAATGGTGCAAGTACAAGGAATGACTGGAAACATCCAATTTGACACTTATGGACGTAGGACAA
  ATTATACCATTGATGTATATGAAATGAAAGTCTCAGGCTCTCGAAAAGCTGGTTACTGGAATGAGTATGA
  AAGGTTTGTGCCCTTCTCAGATCAACAAATCAGCAATGACAGCTCATCCTCCGAGAACAGGACCATCGTA
  GTGACTACCATTCTGGAATCACCATATGTAATGTATAAAAAGAATCATGAGCAACTGGAAGGAAATGAGC
  GCTATGAAGGCTATTGTGTCGATTTAGCCTATGAAATAGCCAAACACGTAAGGATCAAATACAAATTGTC
  CATTGTCGGTGATGGGAAATATGGCGCAAGGGATCCAGAGACTAAAATATGGAATGGCATGGTTGGGGAA
  CTTGTCTATGGAAGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGGTCCGTGAAGAAGTCATAG
  ATTTTTCAAAGCCATTTATGAGCCTGGGGATCTCCATCATGATAAAGAAGCCTCAGAAATCAAAGCCAGG
  CGTATTTTCATTCCTGGATCCTTTAGCTTATGAAATCTGGATGTGCATTGTCTTCGCTTACATTGGAGTC
  AGTGTAGTTCTCTTCCTAGTCAGCAGATTTAGCCCTTATGAGTGGCACTTGGAAGACAACAATGAAGAAC
  CTCGTGACCCACAAAGCCCTCCTGATCCTCCCAATGAATTTGGAATATTTAACAGTCTTTGGTTTTCCTT
  GGGTGCTTTTATGCAGCAAGGATGTGATATTTCTCCAAGATCACTTTCTGGGCGCATTGTTGGAGGGGTT
  TGGTGGTTCTTCACCCTGATCATAATCTCTTCCTACACTGCAAACCTTGCTGCTTTCCTGACTGTGGAGA
  GGATGGTGTCCCCCATAGAGAGCGCTGAAGATTTAGCCAAGCAGACTGAAATTGCATACGGGACCCTGGA
  CTCTGGTTCAACAAAAGAATTTTTCAGAAGATCCAAAATTGCTGTGTATGAGAAAATGTGGTCTTACATG
  AAATCCGCAGAGCCATCTGTGTTTACCAAAACAACAGCTGATGGGGTAGCCCGAGTTCGGAAGTCCAAGG
  GAAAGTTCGCCTTCCTGCTGGAGTCAACCATGAATGAGTACATTGAGCAGAGAAAGCCGTGTGATACGAT
  GAAAGTTGGTGGAAATCTGGATTCCAAAGGCTATGGTGTGGCAACCCCTAAAGGCTCAGCATTAGGAACG
  CCTGTAAACCTTGCAGTATTGAAACTCAGTGAACAAGGCATCTTAGACAAGCTGAAAAACAAATGGTGGT
  ACGATAAGGGGGAATGTGGAGCCAAGGACTCCGGGAGTAAGGACAAGACCAGTGCTCTAAGCCTGAGCAA
  TGTGGCAGGCGTGTTCTATATACTTGTCGGAGGTCTGGGGCTGGCCATGATGGTGGCTTTGATAGAATTC
  TGTTACAAATCACGGGCAGAGTCCAAACGCATGAAACTCACAAAGAACACCCAAAACTTTAAGCCTGCTC
  CTGCCACCAACACTCAGAATTACGCTACATACAGAGAAGGCTACAACGTGTATGGAACAGAAAGTGTTAA
  GATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016886
• Insert Size: 2667 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC086678, AAH86678
• RefSeq Size: 5213 bp
• RefSeq ORF: 2667 bp
• Locus ID: 53623
• UniProt ID: Q9Z2W9
• Cytogenetics: X 23.19 cM
• Gene Summary: This gene encodes a multi-pass transmembrane protein that forms a homotetramer or heterotetramer in neuronal cells. The encoded protein is a ligand-gated ion channel that responds to the neurotransmitter L-glutamate to promote synaptic transmission. Deficiency of this gene leads to behavioral phenotypes. The transcript is subject to RNA editing at codon 769 (AGA->GGA; R->G). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (1) encodes isoform a. Both variants 1 and 2 encode isoform a.