Exosc10 (NM_016699) Mouse Untagged Clone

CAT#: MC222420

Exosc10 (untagged) - Mouse exosome component 10 (Exosc10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Exosc10
• Synonyms: p2; p3; p4; PM-Scl; PM/Scl-100; Pmscl2; RRP6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222420 representing NM_016699
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCTCCCAGTCCCCGGGAGCATCAGTCCGCGCCGGCGACCAGTGCGACCAAGCCTGACGCGGAGA
  TGGTACTGCCTGGCTTCCCCGATGCAGACAGCTTCGTAAAGTTTGCACTTGGGTCGGTAGTGGCAGTTAC
  CAAGGCATCCGGGGGCCTGCCACAGTTCGGTGACGAGTATGATTTTTACAGAAGTTTCCCTGCCTTCCAG
  GCATTCTGTGAGACACAAGGAGACAGGTTACTGCAGTGCATGAGTCGGGTAATGCAGTACCATGGCTGTC
  GCAGCAACATCAAAGACCGAAGTAAAGTGACTGAATTGGAGGACAAGTTTGATTTATTAGTCGATACCAA
  TGACGTGATATTGGAGAGAGTGGGCATGTTACTGGATGAAGCCTCCGGTGTGAACAAGCATCAGCAGCCT
  GTCCTTCCTGCAGGGCTGCAGGTCCCCAAAACAATAGTATCCAGCTGGAATCGGAAGGCAGGAGAGTATG
  GCAAAAAGGCAAAATCGGAGACTTTCCGACTGCTGCATGCAAAAAACATCGTGCGACCTCAGCTCAGGTT
  TCGAGAGAAGATCGACAATTCTAACACACCATTTCTCCCGAAGATCTTTGTCAAACCCAATGCCCGGAAG
  CCACTCCCTCTGGCTCTCTCAAAAGAAAGGCGGGAACGCCCGCAGGACCGTCCGGAGGACCTGGATGTCC
  CCCCAGCCCTGGCAGATTTCATCCATCAGCAGCGAACCCAGCAGGTGGAGCAGGACATGTTTGCACACCC
  TTACCAGTATGAACTGGATCACTTTACTCCGCCTCAGTCGGTGCTGCAGAGGCCGAAGCCCCAGTTGTAC
  CGAGCTGTGGGAGAGACTCCCTGCCACTTGGTGTCGTCCCTGGATGAGCTGGTGGAGCTCAACGAGAAGC
  TCCTGGGCTGTCAGGAGTTTGCCGTGGACTTAGAGCATCACTCTTACAGAAGCTTCCTAGGACTCACCTG
  CCTCATGCAGATCTCCACCCGGACAGAAGACTTCATTGTCGACACCCTCGAGCTTCGCAGTGACATGTAC
  ATTCTCAACGAGAGCCTCACGGACCCAGCCATCGTTAAGGTCTTCCATGGTGCCGACTCTGACATTGAGT
  GGCTGCAGAAAGACTTCGGACTCTATGTGGTGAACATGTTTGACACACACCAGGCAGCACGGCTTCTCAA
  CCTGGCTCGGCACTCACTCGACCATCTGCTGAGACTCTACTGCGGTGTGGAATCAAACAAGCAATATCAG
  CTGGCAGACTGGAGGATACGTCCTCTGCCAGAGGAAATGCTGAGCTACGCCCGGGATGACACCCATTACC
  TGCTCTACATCTATGACCGAATGAGGCTGGAACTGTGGGAAAGAGGCAACCACCAGCCTGTCCAGTTGCA
  GGTGGTTTGGCAGCGGAGCAGGGACATCTGCCTCAAGAAATTTGTCAAGCCTATCTTTACGGACGAGTCC
  TACCTGGAGCTCTATCGAAAGCAAAAGAAGCACCTGAACTCGCAGCAGCTGACCGCCTTCCAGCTGCTGT
  TTGCTTGGAGGGATAAGACAGCTCGCAGGGAGGATGAGAGCTACGGATATGTTCTACCAAATCACATGAT
  GCTAAAGATAGCTGAGGAACTGCCTAAGGAGCCTCAAGGCATCATAGCTTGCTGTAACCCAGTACCACCT
  CTTGTCCGGCAGCAGATCAATGAGATGCATCTCCTAATCCAGCAGGCTCGAGAGATGCCCCTGCTCAAGT
  CTGAAAATGCAGCTGGAGTGAGGAAGAGCGGACCACTGCCTAGCGCCGAGAGATTGGAGAATGATCTCTT
  TGGACCCCATGACTGTTCCCACGCCCCTCCAGATAACTACCAGAACACCTCAACTGATGGGACCCTGCCA
  CTTCAGAAGCAGCCAAGCCTATTCACTGAGGGCAAAGAAGAGACCTCTGTGGATGCCGGATGCCTCCTTG
  CCACAGCCGTCATCACTCTGTTCAGTGAACCTAACACTGAGGAAGGTGGAAAGACTCCATTGACGGTGGC
  CCAGAAAAAAGCCCAGAACATAATGCAGTCCTTTGAAAACCCATTTCGGATGTTCCTGCCTTCTCTGGAA
  CACAAGGCCCACATCTCTCAAGCAGCAAAGTTTGATCCTTCTTCGAAAATCTATGAAATCAGCAACCGCT
  GGAAGCTGGCCAGCCAGGTCCAGGTCCAGAAAGAACCTAAAGAAGCAACCAAGAAGAAGGTAGCTGAGCA
  AACAGCTGCCCGGGAGGAGGCGAAGGAGGAGGCTGCAGCCGGTGTGCTGGAACAGGCTATCCCTGTGCGG
  CAGCAGGCCGCGCTAGAAAATGCTACTAAGAAGAGAGAACGAGCCACCAGTGACCTGAGGACTATAGAGC
  AAAAACAGGAGAAGAAACGACTAAAAAGCTCCAAGAAAGCCAAGGACCCTGATCCCCCAGGGAAAGACTT
  CAGTCCCTATGACTACAGCCAGTCAGACTTCAGGGCCTTTGCTGGGGACAGTAAATCCAAGCCTTCCTCC
  CAGTTCGACCCCAATAAGCTGGCACCTTCCGGCAAGAAAGGTGTTGGAGCCAAAAAATGTAAACAGTCGG
  TGGGAAACAAAAGCATGTCATTTGCTGTTGGAAAGTCGGACAGAGGCTTCCGCCACAACTGGCCAAAGAG
  ATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016699
• Insert Size: 2664 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016699.2, NP_057908.2
• RefSeq Size: 2803 bp
• RefSeq ORF: 2664 bp
• Locus ID: 50912
• UniProt ID: P56960
• Cytogenetics: 4 E2
• Gene Summary: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of MTREX, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).