Ect2 (NM_001177625) Mouse Untagged Clone

CAT#: MC222366

Ect2 (untagged) - Mouse ect2 oncogene (Ect2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ect2
• Synonyms: AI528536; mKIAA4037
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222366 representing NM_001177625
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACGACAGCGTGTTACCATCTCCTTCTGAGATAACTAGCTTGGCAGACTCTTCAGTGTTTGATT
  CTAAAGTTGCTGAAATGTCCAAGGAAAACTTGTGCTTGGCGTCTACTTCAAATGTTGATGAAGAAATGCC
  GCAGGTTGAAGCAAGAGTGATAATGGTCCAGGATGCTGGGAAACAAGAAGAACTTCTAAAGGCCTTAAAG
  GAAATGAAAGTGCCCTGTGTAAAGATGGACTCAATGGAAGAATTTGAAAGTTTGGATTCCCCAGAATTTG
  AGAATATATTTGTAGTTACTGACTTCCAGAATTCTGTCTTCAATGACTTATACAAGGCTGATTGTAGGAT
  TGTTGGGCCACCAGTTATACTCAACTGTGCACAAAGGGGAGAGCCCTTGCCGTTTTCCTGCCGGCCGCTG
  TATTGTACGAGTATGCTGAACCTGGTGCTCTGCTTCACTGGATTCAGGAAGAAGGAGGAGCTTGTCAAAT
  TGGTGACGTTGGTTCATCATATGGGTGGAGTTATTCGAAAAGAGTGTAATTCCAAAGTAACACATCTGGT
  GGCAAATTGTACACAAGGCGAAAAATTTAGGGTTGCTGTGAGCCTGGGCACTCCAATTATGAAGCCAGAA
  TGGATTTATAAAGCGTGGGAAAGACGCAATGAACAGTGTTTCTGTGCAGCAGTTGATGACTTTAGAAATG
  AATTTAAAGTTCCTCCATTCCAAGATTGCATTTTAAGTTTCCTGGGATTTTCAGATGAAGAGAAACATAG
  TATGGAAGAAATGACTGAAATGCAAGGAGGTAGCTATTTACCAGTTGGGGATGAAAGGTGCACTCACCTT
  ATTGTTGAAGAGAATACAGTAAAGGACCTTCCATTCGAACCTTCAAAGAAACTTTTTGTTGTCAAGCAAG
  AGTGGTTCTGGGGAAGCATTCAGATGGACGCTCGTGCAGGAGAGACTATGTATTTGTATGAAAAGGCTAA
  TACTCCTGAGCTCAAGAAATCGGTGTCTCTGCTTTCTCTAAGTACTCCAAACAGCAACCGCAAGAGACGG
  AGATTGAAAGAGACCCTGGCTCAGCTCTCCAGGGAGACTGACCTCTCTCCTTTCCCTCCCCGGAAGCGGC
  CCTCAGCTGAGCACTCTCTTTCTATTGGCTCACTCCTGGATATCTCCAACACACCCGAGTCCAGCATCCA
  CTATGGAGAAACACCGAAGTCCTGTGCTAAGTCTTCTAGAAGTTCTACTCCAGTTCCTCCAAAGCAGTCA
  GCCAGGTGGCAGGTGGCGAAAGAGCTCTACCAGACTGAGAGCAATTATGTCAATATACTGGCCACAATCA
  TTCAATTATTTCAGGTGCCCTTAGAAGAAGAAGGACAGCGTGGTGGGCCTATTCTTGCACCTGAGGAGAT
  CAAGACTATTTTTGGGAGCATCCCAGATATCTTTGATGTGCATATGAAGATCAAGGATGATCTTGAAGAC
  CTTATTGCTAACTGGGATGAGAGCAGAAGTATTGGTGACATCTTTCTTAAATATGCAAAAGATTTGGTAA
  AAACCTACCCTCCGTTTGTAAACTTCTTTGAAATGAGCAAGGAAATGATCATTAAATGTGAGAAACAAAA
  GCCCAGATTTCATGCTTTCCTCAAGATAAATCAAGCTAAACCAGAATGTGGACGACAGAGCCTTGTGGAA
  CTTCTCATCCGGCCAGTGCAGAGGCTACCCAGTGTCGCTTTACTTTTAAACGATCTTAAAAAGCATACAG
  CTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCACTAAAAGAAGTAATGACACATAT
  CAATGAGGATAAGAGAAAAACAGAAGCACAGAAGCAAATTTTTGATGTTGTTTATGAAGTAGATGGATGC
  CCAGCTAATCTCTTATCTTCTCATCGGAGCCTGGTGCAACGAGTGGAAACGGTTTCCCTTGGTGAGCACC
  CTTGCGACCGAGGAGAACAAGTCACTCTCTTCCTCTTCAATGACTGCCTCGAGATAGCAAGAAAGCGGCA
  CAAGGTTATTGGCACTTTTAGAAGTCCTCACGACCGCACCCGGCCCCCCGCTTCTCTGAAGCACATTCAT
  CTCATGCCTCTTTCTCAGATTAAGAAGGTGCTGGACATCCGAGAGACAGAAGATTGTCACAATGCCTTTG
  CCTTGCTTGTAAGGCCACCAACAGAACAGGCCAATGTACTGCTCAGCTTCCAGATGACGTCAGAGGAGCT
  TCCAAAGGAGAGCTGGCTGAAGATGCTGTGCCGACATGTAGCCAACACCATTTGTAAGGCAGATGCTGAG
  AATCTTATGTATGTGGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGATAGTACATTGAGTC
  GAGCATCTAGAGCAATAAAGAAGACTTCAAAAAAGGTTACAAGGGCATTCTCTTTTTCCAAAACTCCCAA
  GAGAGCCCTGCGGATGGCTCTTTCATCATCCCATAGCTCAGAGGGAAGGAGTCCTCCGAGCAGTGGCAAG
  CTTGCTGTGAGCCGTCTGTCCAGCACATCATCCTTAGCAGGTATTCCATCTCCCTCCCTTGTCAGCCTCC
  CTTCTTTCTTTGAACGGAGAAGTCATACATTAAGTCGATCTACAACTCACTTGATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177625
• Insert Size: 2649 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177625.1, NP_001171096.1
• RefSeq Size: 4002 bp
• RefSeq ORF: 2649 bp
• Locus ID: 13605
• UniProt ID: Q07139
• Cytogenetics: 3 A3
• Gene Summary: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) is missing an in-frame coding exon compared to variant 1, resulting in a shorter isoform (2) lacking an internal protein segment compared to isoform 1. Variants 2 and 3 encode the same isoform.