Kcnq3 (NM_152923) Mouse Untagged Clone

CAT#: MC222342

Kcnq3 (untagged) - Mouse potassium voltage-gated channel, subfamily Q, member 3 (Kcnq3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnq3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222342 representing NM_152923
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTCAAGGCTCGCAGGGCGGCGGGGGCGGCTGGCGGGGGCGGCGGCGAAGGGGGCGGCGGCGGCG
  GCGGGGCCGCTAACCCTGCTGGAGGGGACTCGGCGGTGGCCGGCGACGAGGAGCGGAAAGTGGGGCTGGC
  GCCAGGAGACGTGGAGCAAGTCACCTTGGCGCTAGGGGCCGGAGCCGACAAAGACGGGACCCTGCTGCTG
  GAGGGCGGTGGCCGCGAAGAGGGGCAGAGGAGGACCCCGCAGGGCATCGGGCTCCTGGCAAAGACCCCCC
  TGAGCCGCCCAGTCAAGAGGAACAACGCCAAGTACAGGCGCATCCAAACTTTGATCTATGACGCCCTGGA
  GAGACCGCGGGGCTGGGCGCTGCTCTACCACGCGCTTGTGTTCCTGATTGTCCTGGGATGCTTGATTCTG
  GCCGTGCTCACCACCTTCAAGGAATATGAGACTGTGTCTGGAGACTGGCTTTTGCTGCTGGAAACCTTTG
  CTATTTTCATCTTTGGAGCTGAGTTTGCTTTGAGGATCTGGGCTGCAGGATGTTGCTGTCGATACAAAGG
  CTGGCGTGGACGGCTAAAGTTTGCCAGGAAGCCCCTGTGCATGTTGGACATCTTCGTACTGATTGCCTCT
  GTGCCAGTGGTTGCCGTGGGAAACCAGGGCAATGTCTTGGCCACCTCCCTGCGAAGCCTTCGCTTCCTGC
  AGATCCTGCGCATGCTTCGAATGGATAGGAGGGGTGGCACCTGGAAGCTCCTGGGCTCGGCTATCTGTGC
  CCACAGCAAAGAACTCATCACTGCCTGGTACATAGGCTTCCTGACACTCATCCTTTCTTCATTTCTTGTC
  TACCTGGTGGAGAAGGATGTGCCAGAGATGGATGCCCAAGGAGAGGAAATGAAGGAGGAGTTTGAGACCT
  ATGCAGATGCTCTGTGGTGGGGCCTGATCACACTGGCCACCATTGGTTATGGAGACAAGACACCTAAAAC
  CTGGGAAGGACGTCTGATTGCTGCCACCTTTTCTTTAATCGGCGTCTCCTTTTTTGCCCTTCCGGCAGGC
  ATCCTTGGCTCAGGACTGGCACTGAAGGTTCAGGAGCAGCACCGTCAGAAGCACTTTGAGAAGAGAAGGA
  AGCCAGCTGCGGAACTCATCCAGGCTGCCTGGAGATATTATGCTACCAACCCCAACAGGTTGGATCTGGT
  GGCAACCTGGAGATTTTATGAATCAGTTGTCTCTTTCCCATTCTTCAGGAAAGAACAACTGGAAGCAGCA
  GCCAGCCAAAAGCTGGGTCTCTTGGATCGGGTTCGCCTTTCTAATCCTCGTGGTAGCAATACTAAAGGAA
  AGCTATTTACCCCTCTGAATGTAGATGCCATAGAAGAAAGCCCTTCCAAAGAGCCAAAGCCTGTTGGCTT
  AAACAATAAAGAGCGTTTCCGCACCGCCTTCCGCATGAAAGCCTACGCTTTCTGGCAGAGTTCTGAAGAT
  GCTGGGACAGGCGACCCCATGGCAGAAGACAGGGGCTATGGGAATGACTTCCTCATTGAAGACATGATCC
  CTACCCTAAAGGCTGCCATCCGAGCTGTCAGAATTCTACAGTTCCGTCTATATAAAAAAAAGTTCAAGGA
  GACGTTGAGGCCTTATGATGTGAAAGATGTGATTGAGCAGTATTCGGCCGGACATCTTGACATGCTTTCC
  AGGATAAAGTACCTACAGACAAGAATAGATATGATTTTCACCCCTGGACCTCCATCCACTCCAAAACATA
  AGAAGTCTCAGAAAGGGTCAGCATTTACCTACCCATCCCAGCAGTCTCCAAGGAATGAACCATATGTAGC
  CAGGGCAGCCACATCAGAAACTGAAGACCAAAGCATGATGGGGAAGTTTGTAAAAGTTGAAAGACAGGTT
  CATGACATGGGGAAGAAACTGGACTTCCTCGTGGACATGCATATGCAGCATATGGAACGCTTACAGGTAC
  ATGTCACTGAGTACTACCCAACTAAGGGGGCCTCCTCCCCAGCCGAAGGGGAGAAGAAAGAAGACAACAG
  GTACTCTGATTTGAAAACCATCATCTGCAACTACTCAGAGACAGGGCCCCCTGACCCTCCTTACAGCTTC
  CACCAGGTGCCCATCGACAGAGTTGGCCCTTACGGGTTTTTTGCACATGATCCTGTGAAACTGACCCGAG
  GGGGACCCAGTTCTACAAAGGCTCAAGCTAACCTTCCCTCCTCGGGAAGTACATATGCAGAGAGGCCCAC
  AGTCCTGCCCATCTTGACTCTTCTGGACTCATGTGTGAGCTACCACTCCCAGACAGAACTGCAAGGTCCC
  TATTCGGACCACATCTCGCCCCGCCAGAGACGCAGCATCACTAGGGACAGTGATACACCTCTGTCCCTCA
  TGTCCGTCAATCACGAGGAACTGGAGCGGTCTCCAAGTGGCTTCAGCATCTCACAAGACAGAGATGATTA
  TGTATTTGGCCCCAGTGGGGGATCGAGCTGGATGAGGGAAAAGCGGTACCTGGCTGAAGGAGAAACGGAC
  ACGGACACAGACCCCTTCACACCCAGTGGTTCCATGCCTATGTCATCCACTGGGGATGGTATTTCAGATT
  CCATATGGACCCCTTCCAACAAGCCCACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_152923
• Insert Size: 2622 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152923.2, NP_690887.2
• RefSeq Size: 2878 bp
• RefSeq ORF: 2622 bp
• Locus ID: 110862
• UniProt ID: Q8K3F6
• Cytogenetics: 15 29.16 cM
• Gene Summary: Associates with KCNQ2 or KCNQ5 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Therefore, it is important in the regulation of neuronal excitability.[UniProtKB/Swiss-Prot Function]