Sfmbt1 (NM_001166532) Mouse Untagged Clone

CAT#: MC222264

Sfmbt1 (untagged) - Mouse Scm-like with four mbt domains 1 (Sfmbt1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sfmbt1
• Synonyms: 4930442N21Rik; 9330180L21Rik; AA536974; Sfmbt; Smr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222264 representing NM_001166532
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGGAGCAGCAGCTTGATGCAGATCTTGGGTCTGGTGTAGAAGTAGAAGAATTCAGCTGGGAAG
  ATTATCTAGAAGAGACAGGCTCCACCACAGTTCCCTACGCATCGTTTAAACATGTGGATATACGTTTGCA
  AAATGGATTTGCTCCTGGGATGAAGCTGGAGGTAGCTCTGAAAAATGACCCTGAGACTTATTGGGTTGCC
  ACCATCATCACCGCCTGTGAGCAGTTGCTCCTGCTTCGTTATGAAGGCTATGGGGAAGACCGAAAAGCAG
  ACTTCTGGTGTGACATCAGGAAAGCTGGCCTCTACCCCATTGGGTGGTGTCAGCAGAACAAGAAGACCCT
  CGAAGCTCCAGAAGGCATCAGAGATAAAGTGTCTGATTGGAATGCATTCCTACAGCAGACCCTGATAGGA
  GCCTGTGGTCCTCCCGTGTCTCTGCTAGAGGGCCTCCGTAATGGAAGGAATCCTTTAGATCTCATTGCTC
  CAGGGTCCAAACTAGAATGCCAGGATTTTCGGGACTCTTTAAGCACTTGGCTTGTTACTGTTGTGGAGAA
  CATTGGGGGACGGTTGAAGCTGCGGTATGAAGGCCTTGAGAGCCGTGACGGTTTTGAACACTGGTTGTAT
  TACTTAGATCCATTTCTTCATCACATTGGTTGGGCTGCTCAGCAAGGATGTGACCTTCAGCCCCCATTAG
  CCATCAAACATCTGAAGAGTGAAGCTGACTGGCAAGAGATTTTGGCCAAAGTGAAAGAGGAAGAGCCATT
  ACCCTCTTACTTATTTAAGGATAAGCAAGTTATTGGAACCCATGAATTTTCTATAAACATGAAGTTGGAA
  GCTGTGGACCCGTGGTCTCCTTTTGGGATTTCTCCTGCAACGATTGCTAAGGTGTTTGATGATAAGTACT
  TTCTAGTGGAAATGGATGACCTTCGGCCAGAAGACCATACAAGGCGATCATTTGTGTGCCATGCTAACAG
  TCCTGGTATCTTTCCTGTGCAGTGGAGTCTAAAGAATGGTTTACATATCAATCCTCCTCCAGGCTTCCGG
  AGCCAGGACTTTGACTGGGCCGACTACCTCAAGCAGTGTGGGGCTGAAGCTGCTCCCCAGAAGTGCTTCC
  CGCAGTCAATTTCTGAGCACCAGTTTAAGGAGAACATGAAACTGGAGGCAGTGAACCCTCTTTTCCCTGA
  AGAAGTGTGCATTGCCACCGTTACTGCAGTGAGAGGCTCGTACCTGTGGCTCCAGCTAGAAGGTTCTAAG
  AAGCCTGTACCCGAATTTATTGTAAGTGCAGAGTCGATGAATATATTTCCTTTGGGCTGGTGTGAAACCA
  ATGGCCATCCTCTCAGTACTCCTCGCCGGGCACGAGGGCATAAACTGAGGAAAATCGCAGTGGTTCAACC
  AGAAAAACAAATACTGTCTTCAAGAACTGTCCACGAGGGCCTGAAGAATCAGCTGAACTCTACCCACTCA
  GTGATGATCAATGGAAAATACTGTTGTCCAAAGATATACTTCAACCACCGTTGCTTCTCAGGGCCATATC
  TTAATAAAGGAAGAATTGCTGAGCTGCCTCAATGTGTGGGACCTGGGAACTGCGTTCTGGTACTTAGAGA
  GGTTCTTACTTTGCTTATCAACGCAGCATACAAACCCAGCCGCGTCCTCCGTGAGCTCCAGCTGGACAAA
  GACTCTGTGTGGCACGGATGTGGGGAAGTCCTAAAGGCCAAATATAAAGGAAAGAGTTACCGAGCTACTG
  TTGAGATAGTGAGAACAGCAGACCGAGTGACTGAATTCTGCCGGCAAACATGCATCAAGCTGGAATGTTG
  TCCCAACCTCTTTGGTCCACGCATGGTTCTGGATACATGTTCTGAGAATTGCTCTGTCCTTACAAAGACC
  AAATACACGCACTATTATGGAAAGAAGAAAAATAAGAGAATTGGGAGGCCACCCGGTGGGCATAGTAATT
  TATCTTGTGCCCTGAAAAAAAGCAGTAAGAGGAGAAAAAGGCGGAAGAATATTTTTGTTCATAAGAAGAA
  GCGTTCTTCTGCATCTGTTGACAATACCCCGGTGGGCTCACCCCAGGGAAGTGGGGGTGAAGATGAGGAG
  GATGCAGATGATGGAGACGAAGACTCCCTGACTGAGGGCAGTACATCCGAGCAGCAGGAGGAGTTGCAGG
  AGGAGTCTGAGGTGTCAGAGAAAAAATCTAGCTCCTCGTCTCCCACCCAGAGTGAGACGCCCACCCCCTT
  ACCCCCAGACACGCAGACCAACAAGAGAGATGCCCAGACCTCCTCCGTCTCAGACGATGAAAACAAGCCT
  CCTTCACCAAAGGAAATAAGGATTGAAGTTGATGAAAGGCTTCACCTGGACAGTAACCCACTGAAGTGGA
  GTGTGGCCGATGTCGTGCGGTTCATCAGGTCCACTGACTGTGCTCCGTTGGCAAGAATATTCCTTGATCA
  GGAAATCGACGGGCAGGCCCTTTTGCTCCTTACCCTTCCCACTGTTCAAGAGTGCATGGACTTAAAGTTG
  GGCCCCGCCATCAAGCTTTGCCATCACATAGAGAGAATCAAGTTTGCTTTTTATGAGCAGTTTGCCAACT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166532
• Insert Size: 2592 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166532.1, NP_001160004.1
• RefSeq Size: 7837 bp
• RefSeq ORF: 2592 bp
• Locus ID: 54650
• UniProt ID: Q9JMD1
• Cytogenetics: 14 18.99 cM
• Gene Summary: Histone-binding protein, which is part of various corepressor complexes. Mediates the recruitment of corepressor complexes to target genes, followed by chromatin compaction and repression of transcription. Plays a role during myogenesis: required for the maintenance of undifferentiated states of myogenic progenitor cells via interaction with MYOD1. Interaction with MYOD1 leads to the recruitment of associated corepressors and silencing of MYOD1 target genes. Part of the SLC complex in germ cells, where it may play a role during spermatogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 all encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.