Hcn2 (NM_008226) Mouse Untagged Clone

CAT#: MC222256

Hcn2 (untagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 2 (Hcn2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hcn2
• Synonyms: BCNG2; HAC1; trls
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222256 representing NM_008226
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCGCGCGGGGGCGGCGGGCGGCCGGGCGATAGTCCGGGCACGACCCCTGCGCCGGGGCCGCCGC
  CACCGCCGCCGCCGCCCGCGCCCCCTCAGCCTCAGCCACCACCCGCGCCACCCCCGAACCCCACGACCCC
  CTCGCACCCGGAGTCGGCGGACGAGCCCGGCCCGCGCGCCCGGCTCTGCAGCCGCGACAGCGCCTGCACC
  CCTGGCGCGGCCAAGGGCGGCGCGAATGGCGAGTGCGGGCGCGGGGAGCCGCAGTGCAGCCCCGAGGGCC
  CCGCGCGCGGCCCCAAGGTTTCGTTCTCATGCCGCGGGGCGGCCTCCGGGCCCTCGGCGGCCGAGGAGGC
  GGGCAGCGAGGAGGCGGGCCCGGCGGGTGAGCCGCGCGGCAGCCAGGCTAGCTTCCTGCAGCGCCAATTC
  GGGGCGCTTCTGCAGCCCGGCGTCAACAAGTTCTCCCTGCGGATGTTCGGCAGCCAGAAGGCCGTGGAGC
  GCGAGCAGGAACGCGTGAAGTCGGCGGGGGCCTGGATCATCCACCCCTACAGCGACTTCAGGTTCTACTG
  GGACTTCACCATGCTGTTGTTCATGGTGGGAAATCTCATTATCATTCCCGTGGGCATCACTTTCTTCAAG
  GACGAGACCACCGCGCCCTGGATCGTCTTCAACGTGGTCTCGGACACTTTCTTCCTCATGGACTTGGTGT
  TGAACTTCCGCACCGGCATTGTTATTGAGGACAACACGGAGATCATCCTGGACCCCGAGAAGATAAAGAA
  GAAGTACTTGCGTACGTGGTTCGTGGTGGACTTCGTGTCATCCATCCCGGTGGACTACATCTTCCTCATA
  GTGGAGAAGGGAATCGACTCCGAGGTCTACAAGACAGCGCGTGCTCTGCGCATCGTGCGCTTCACCAAGA
  TCCTCAGTCTGCTGCGGCTGCTGCGGCTATCACGGCTCATCCGATATATCCACCAGTGGGAAGAGATTTT
  CCACATGACCTACGACCTGGCAAGTGCAGTGATGCGCATCTGTAACCTGATCAGCATGATGCTACTGCTC
  TGCCACTGGGACGGTTGCCTGCAGTTCCTGGTGCCCATGCTGCAAGACTTCCCCAGCGACTGCTGGGTGT
  CCATCAACAACATGGTGAACCACTCGTGGAGCGAGCTCTACTCGTTCGCGCTCTTCAAGGCCATGAGCCA
  CATGCTGTGCATCGGCTACGGGCGGCAGGCGCCCGAGAGCATGACAGACATCTGGCTGACCATGCTCAGC
  ATGATCGTAGGCGCCACCTGCTATGCCATGTTCATTGGGCACGCCACTGCGCTCATCCAGTCCCTGGATT
  CGTCACGGCGCCAATACCAGGAGAAGTACAAGCAAGTAGAGCAATACATGTCCTTCCACAAACTGCCCGC
  TGACTTCCGCCAGAAGATCCACGATTACTATGAACACCGGTACCAAGGGAAGATGTTTGATGAGGACAGC
  ATCCTTGGGGAACTCAACGGGCCACTGCGTGAGGAGATTGTGAACTTCAACTGCCGGAAGCTGGTGGCTT
  CCATGCCGCTGTTTGCCAATGCAGACCCCAACTTCGTCACAGCCATGCTGACAAAGCTCAAATTTGAGGT
  CTTCCAGCCTGGAGATTACATCATCCGAGAGGGGACCATCGGGAAGAAGATGTACTTCATCCAGCATGGG
  GTGGTGAGCGTGCTCACCAAGGGCAACAAGGAGATGAAGCTGTCGGATGGCTCCTATTTCGGGGAGATCT
  GCTTGCTCACGAGGGGCCGGCGTACGGCCAGCGTGCGAGCTGACACCTACTGTCGCCTCTACTCACTGAG
  TGTGGACAATTTCAACGAGGTGCTGGAGGAATACCCCATGATGCGGCGTGCCTTTGAGACTGTGGCTATT
  GACCGGCTAGATCGCATAGGCAAGAAGAACTCCATCTTGCTGCACAAGGTTCAGCATGATCTCAGCTCAG
  GTGTGTTCAACAACCAGGAGAATGCCATCATCCAGGAGATTGTCAAATATGACCGTGAGATGGTGCAGCA
  GGCAGAGCTTGGCCAGCGTGTGGGGCTCTTCCCACCACCGCCACCACCGCAGGTCACATCGGCCATTGCC
  ACCCTACAGCAGGCTGTGGCCATGAGCTTCTGCCCGCAGGTGGCCCGCCCGCTCGTGGGGCCCCTGGCGC
  TAGGCTCCCCACGCCTAGTGCGCCGCGCGCCCCCAGGGCCTCTGCCTCCTGCAGCCTCGCCAGGGCCACC
  CGCAGCAAGCCCCCCGGCTGCACCCTCGAGCCCTCGGGCACCGCGGACCTCACCCTACGGTGTGCCTGGC
  TCTCCGGCAACGCGTGTGGGGCCCGCATTGCCCGCACGTCGCCTGAGCCGCGCCTCGCGCCCACTGTCCG
  CCTCGCAGCCCTCGCTGCCCCATGGCGTGCCCGCGCCCAGCCCCGCGGCCTCTGCGCGCCCGGCCAGCAG
  CTCCACGCCGCGCCTGGGACCCGCACCCACCGCCCGGACCGCCGCGCCCAGTCCGGACCGCAGGGACTCA
  GCCTCGCCGGGCGCTGCCAGTGGCCTCGACCCACTGGACTCTGCGCGCTCGCGCCTCTCTTCCAACTTGT
  GA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_008226
• Insert Size: 2592 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008226.2, NP_032252.1
• RefSeq Size: 3195 bp
• RefSeq ORF: 2592 bp
• Locus ID: 15166
• UniProt ID: O88703
• Cytogenetics: 10 C1
• Gene Summary: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current.[UniProtKB/Swiss-Prot Function]