Pla2g4f (NM_001024145) Mouse Untagged Clone

CAT#: MC222204

Pla2g4f (untagged) - Mouse phospholipase A2, group IVF (Pla2g4f), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pla2g4f
• Synonyms: 4732472I07Rik; Gm647; Pla2zeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222204 representing NM_001024145
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTGGACTCTCCAGCCAAAGTGGCTGGCAGGCAAGGGACTTCCCCTTCTTGGAGCCATACTGCTAC
  GGAAGACAGAAAAGAGCGAACCACAATGGAAGCATAGGCGGCAGGAAACCCACCCATACTACGACCTTCA
  AGTGAAGGTGCTGAGGGCCAGAAACATCCAGCACACAGATAAGTTGTCCAAAGCCGACTGCTATGTTCGA
  CTGTGGCTGCCCACGGCTTCTGTTAGCCCCAGTCAGACAAGGACAGTGGTTAACAGCAGTGATCCAGAAT
  GGAATGAGACCTTTCCCTATCAGATCCACGGCGCTGTGAAGAACGTCCTGGAGCTTGCCCTTTATGACGA
  GGATGTCCTGGACAGTGACAATGTCTTCTCCATTCTGTTTGACACGAGTACTCTTCAGCTAGGCCAGCCT
  TGCACAAAAAACTTCACCAGGCAGCAGGATCCAAAAGAGCTGGAAGTAGAATTTACTCTGGAAAAGAGTC
  AGACGCCTGCATCTGAAGTTGTCACCAATGGTGTCCTGGTGGCTCACCCCTGTCTGAGAATTCAGGGCAC
  AGTCACTGGAGACAAGACAGCCTCCCTTGGAGAGTTGGGCTCCAGGCAGATCCAGCTGGCAGTGCCTGGG
  GCCTATGAAAAGCCACAGCCTCTGCAGCCAACCTCGGAGCCAGGCCTCCCAGTGAACTTTACCTTCCACG
  TGAACCCAGTGCTGAGCCCCAAGCTGCACATAAAGCTGCAAGAACAGCTCCAAGTCTTCCATAGTGGCCC
  GAGTGATGAGCTGGAAGCTCAGACCAGCAAGATGGACAAGGCAAGCATCCTGCTCTCCTCTCTGCCCCTC
  AACGAGGAGTTAACGAAACTTGTGGACCTGGAGGAGGGCCAGCAGGTGTCTCTTAGGATGAAGGCAGACA
  TGAGCAGCTCTGGGGACTTGGACCTGCGCCTTGGTTTTGACCTCTGTGATGGAGAGCAGGAATTTCTGGA
  CAAGAGGAAGCAGGTGGCGTCCAAGGCCCTGCAGCGGGTGATGGGATTGAGTGAGGCTCTGCACTGTGAC
  CAGGTACCTGTGGTAGCCGTGTTAGGCTCTGGGGGTGGAACCAGAGCCATGACTTCCCTGTACGGCAGCC
  TGGCTGGGCTGCAGGAGCTTGGTCTTCTGGATGCCGTGACCTACCTGAGTGGGGTCTCTGGGTCTTCCTG
  GTGCATCTCTACACTCTACAGGGATCCATCCTGGTCCCAGAAGGCTTTGCAGGGCCCCATTAAATATGCC
  TCAGAGCGAGTCTGCAGCAGTAAAATTGGGATGCTGTCCCCAAAGCAGTTTGAATACTACTCCCGGGAAA
  AGAGAGCCTGGGAGAGCAGGGGACACAGCATGTCCTTCACTGACTTGTGGGGCCTCATCATTGAGTATTT
  CCTGAACCAGGAGGAAAACCCTGCCAAGCTGTCAGACCAGCAAGAAACGGTCAGCCAGGGTCAGAACCCA
  TACCCCATCTATGCCAGCATTAATGTCCACAAAAACATCAGTGGGGACGACTTTGCAGAGTGGTGCGAGT
  TCACCCCCTATGAGGTCGGTTTCCCCAAGTACGGGGCTTACGTTCCCACGGAACTCTTTGGCTCTGAATT
  CTTCATGGGCCGGCTGCTGCATTTCTGGCCAGAGCCCCGCATCTGTTACCTGCAGGGTATGTGGGGAAGT
  GCTTTTGCAGCCAGCCTGTATGAGATCTTCCTGAAGCTGGGAGGCCTAAGCCTGAGCTTTCTGGACTGGC
  ACAGGGGGAGTGTCAGTGTCACAGATGACTGGCCAAAGTTACGGAAGCAGGACCCCACACGGCTGCCTAC
  CAGACTCTTCACGCCAATGAGTTCCTTCTCTCAGGCTGTGCTGGACATATTCACCTCCCGTATTACTTGT
  GCCCAGACCTTTAACTTTACCCGAGGTCTCTGCATGTACAAAGACTACACAGCTAGAAAGGACTTCGTGG
  TCTCTGAAGATGCATGGCATTCACATAACTATGGATACCCTGATGCCTGTCCCAACCAGCTCACACCCAT
  GAAGGACTTCCTGTCCCTAGTAGATGGAGGCTTTGCTATCAACTCGCCATTTCCACTGGTCCTGCAGCCG
  CAGCGGGCTGTGGACCTCATTGTGTCCTTTGACTATTCCTTGGAAGGCCCTTTTGAGGTCCTGCAGGTGA
  CAGAGAAGTACTGCCGGGACCGAGGGATCCCCTTCCCAAGGATTGAGGTGGACCCCAAGGACTCTGAAGA
  CCCCCGTGAATGCTATCTGTTTGCTGAGGCAGAGGACCCCTGCTCGCCCATCGTGCTGCATTTCCCTCTT
  GTCAACAGGACCTTTCGCACGCACCTGGCCCCAGGTGTGGAACGACAAACAGCTGAGGAGAAGGCCTTCG
  GGGACTTTATCATCAACGGGCCAGATACTGCCTATGGCATGATGGATTTCACCTACGAGCCCAAGGAATT
  TGATCGGCTGGTGACCCTGAGCCGATACAACGTCTTGAACAACAAGGAGACTATCAGGCATGCCCTCCAG
  CTGGCTCTGGACCGGCGGCGGCAGGCTGGGGGAAGGGTTGGGGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024145
• Insert Size: 2568 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024145.2, NP_001019316.1
• RefSeq Size: 3125 bp
• RefSeq ORF: 2568 bp
• Locus ID: 271844
• UniProt ID: Q50L41
• Cytogenetics: 2 E5
• Gene Summary: Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.