Myrip (NM_144557) Mouse Untagged Clone

CAT#: MC222202

Myrip (untagged) - Mouse myosin VIIA and Rab interacting protein (Myrip), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myrip
• Synonyms: A230081N12Rik; Slac2-c; Slac2c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222202 representing NM_144557
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGGAAGCTGGACCTGTCGGGTCTGACCGACGATGAGACCGAGCATGTTCTCCAAGTGGTGCAGA
  GAGACTTCAATCTTCGCAAAAAGGAGGAGGACCGGCTAAGTGAGATGAAGCAGAGGCTGGCCGAGGAGAA
  CAGCAAGTGCAGCATCCTCTCGAAGCACCAGAAGTTCGTGGAGCGCTGCTGCATGCGGTGCTGCTCGCCC
  TTCACCTTCCTGGTCAACGCCAGGCGCCGCTGCGGGGAATGCAAGTTCAGCGTCTGCAAGAGCTGCTGCT
  CCTACCAGAAGCACGAGAAGCTATGGGTTTGCTGTGTCTGCCAGCAAGCCAGGCTTCTGAGGACACAGTC
  TCTGGAGTGGTTCTACAACAATGTGAAAAGCCGCTTCAAGCGCTTTGGCAGTGCCAAGGTCCTGAAGAAC
  CTGTACAGAAAACACCGTCTGGAGAGCGGTGCCTGCTTCGACATCCTAGGAGGAGGATTGTTTGAACCAA
  ACCTGGAGAATGAAGGGAGCATTTCAGGAAGTGACTCCACGTTCTACAGGCAGTCAGAAGGGCACAGCAT
  GATGGACACCTTGGCTGTGGCCCTACGAGTGGCAGAAGAGGCCATCGAGGAGGCCATTTCCAAAGCAGAA
  TCCCATGGGGACAGCCTGGACAAACAGAACGAGGCCAGCTACCTGCGGGACCACAAGCAAGAGCTAACTG
  AGGAGCTGGCCGGCACCATCCTGCAGAGGATTATAAGAAAGCAGAAGGATAAAGCTGAGCTGCGAGCTGA
  GGAGGAGGAGCCAGAGTGGCCACGGTCCCAGAGTGGCAGTGTGAAGGCCAGGGGTGAGGGGACCACGGCC
  CCTCCAGGACGACACAAAGCACGTGCCACCTTCAGGCGGTCCCAGTCTGCATTCTCCTTCACTATGGAAG
  ATGCTCTGAAGTCTGGTTCAGCAGAGGCTGCACCAAGGTCACCCAAGGACCGCGCCCAGCGTCTGCTGGA
  GGAGGCCGCTCTGCCCAGCTGGAGGAGCATGGATGGACTGGACGGAACAAACCTGGCCCCACTTTTGCAG
  AGCCCCGATGGGAACTGGATGACCTTGAAGGACGGTAGTAGACAGCCCCCAACCCGCCTGCTGACCAAAC
  CGAAGAGTGGGACCTTTCAGGCCCTGGAGGTGGCGTCCAGTGTGACGTCTGCCTATGATGAGATCGGTTC
  CGACAGTGAGGAAGACTTTGATTACAGCGAAGCCTTGAGTAAGCTGTGCCCACCGTCCCAGAGCAGGCTC
  AAGCAACCCCAGCCTCAGCCTACACAGGCCCAGAGTTCTGGCCAAGGTCCCTTAGCCACCTCTCCCTCAA
  ACCCTGAGGCCATGTGCTCGGATTCAGAGACATCTTCCACCAGCTCATCTCGGGAAGCTGGCTGCCGGGC
  TAAGCTGTCCTGGCTGCAGAGGAAGGCCCCCAAGAACCCCGCAGTGGAGAAGATGCCCCTCCAGGGAGAA
  CTGGACGTCAATTTCAACCCTCAGGCAGCCGGTGGAGAGACTTCCGACAGTAGCGATCCAGAGGAGACCC
  TCCGCACTGCAGAGCGCCGGGCCAGGAGGTGGAGAAGGGCACGAGTGGGCCCCGAGGAGTCAAACAGGGG
  TCTGCCTTCACCTGGTGCCCATCCACGGGCACTGCACACGGCTCAGGTGTCAGACAACGTGTCAGAGACT
  GACATCAGCAACGAGACTCAGAATTCCCGGTCCAGCACGGACTCAGTAGAGGAGAAGCTGAGAAATAGAC
  TGTATGAGTTAGCCATGAAAATGAGCGAAAAGGAGACTTCTTCTGGGGAGGACCAGGAGTCCGAGTCCAA
  GGCGGAACCTAAGAACCAGAAGGGAAGTCTCTCTTCTGAGGAGAACAACCAGGGTGTCCAGGAGGAGCTG
  AAGAAGAAGTGTTCTGCTGTTTCTCTCTGCAACATCTCCACAGAAGTCCTGAAGGTCATCAATGCTACCG
  AGGAGTTGATCGCAGAGTCTGCAGGGCCCTGGGAGATCCCCCCAGTCTCCACTGACAGAGAAAACGGGAT
  GTTTCCTCTTGGGACAGACCAAGTGAGACTGGACAAGCAGCTGACGTCCTTGGAAGAAAACGTGTACCTG
  GCAGCGGGCACCGTGTATGGGCTGGAGGGCCAGCTGAGTGAGCTGGAAGACGCTGCCCGCTGCATCCACA
  GCAGCACTGGCGAGACGGAGCTGGCAGACCTGGAGGACCAGGTGGCTGCAGCTGCAGCCCAGGTGCACCA
  CGCCGAACTCCAGATCTCGGACATCGAGAGCCGCATTTCAGCCCTGACCATCGCTGGCTTAAACATAGCA
  CCGTGTGTGCGTCTCACACGAAGACGGGACCAGAAGCAAAGGAGCCAGGTACAAACTATAGACACGTCGA
  GGCAGCAGAGGAGGAAACTGCCTGCCCCTCCTGTGAAAGCTGAAAAAATCGAAGCATCTTCCGTGACCCC
  CATTAAGACATTCAACCGCAACTTCCTTCTCCAAGGCTCCTCCACAAACAGGCCCACAGCAAGTACCGGT
  GACACCAAGGACTTGATGGAGCCTGATCTGGAGTCAGCTGTGATGTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144557
• Insert Size: 2571 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144557.5, NP_653140.1
• RefSeq Size: 4762 bp
• RefSeq ORF: 2571 bp
• Locus ID: 245049
• UniProt ID: Q8K3I4
• Cytogenetics: 9 F4
• Gene Summary: Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments. Functions as a protein kinase A-anchoring protein (AKAP). May act as a scaffolding protein that links PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release.[UniProtKB/Swiss-Prot Function]