Ago1 (NM_153403) Mouse Untagged Clone

CAT#: MC222196

Ago1 (untagged) - Mouse eukaryotic translation initiation factor 2C, 1 (Eif2c1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ago1
• Synonyms: Eif2c; Eif2c1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222196 representing NM_153403
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCGGGACCCTCGGGAGCAGCGGCAGGTGCCTACCTGCCTCCCCTGCAGCAGGTGTTTCAGGCAC
  CCCGCCGGCCTGGCATTGGCACTGTGGGCAAACCAATCAAGCTTCTGGCCAATTACTTTGAGGTGGACAT
  TCCTAAGATTGACGTTTACCATTACGAGGTGGACATCAAGCCGGATAAGTGTCCTCGCAGAGTCAACCGG
  GAGGTGGTGGAATACATGGTCCAGCATTTCAAACCGCAGATCTTTGGGGATCGCAAGCCTGTGTATGATG
  GAAAGAAGAATATTTACACGGTCACAGCACTGCCCATTGGCAATGAGAGGGTTGACTTTGAGGTGACAAT
  CCCCGGGGAGGGGAAGGATAGAATTTTTAAGGTCTCCATCAAGTGGCTAGCCATTGTGAGCTGGCGCATG
  CTGCATGAAGCCTTGGTCAGTGGCCAGATCCCTGTGCCCCTGGAGTCTGTGCAAGCCCTGGATGTGGCCA
  TGAGGCACCTGGCATCTATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTA
  CCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAG
  ATGATGCTCAACATTGATGTCTCAGCCACTGCCTTCTACAAAGCACAGCCAGTGATTGAGTTCATGTGTG
  AGGTCCTGGACATCAGGAACATAGACGAACAACCCAAGCCCCTCACGGATTCCCAGCGTGTTCGGTTTAC
  CAAGGAGATAAAAGGCCTGAAGGTGGAAGTGACCCACTGTGGACAGATGAAGAGGAAATACCGCGTGTGT
  AATGTTACCCGCCGCCCTGCTAGCCATCAGACGTTCCCCTTGCAGCTGGAGAGTGGACAGACTGTGGAGT
  GCACTGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGCTCAAGTATCCTCACCTGCCCTGCCTACA
  AGTTGGGCAAGAACAGAAGCATACCTATTTGCCCCTCGAGGTCTGTAACATTGTGGCTGGGCAGCGGTGC
  ATTAAGAAGCTGACTGACAACCAGACTTCAACCATGATAAAGGCTACAGCTAGGTCGGCCCCAGACAGAC
  AGGAGGAGATCAGCCGCCTGATGAAGAATGCTAGCTACAACCTGGATCCCTACATCCAGGAATTTGGAAT
  CAAAGTGAAGGATGACATGACGGAGGTGACAGGACGTGTGCTGCCGGCGCCCATCTTGCAGTATGGCGGC
  CGGAACCGGGCCATTGCTACGCCCAACCAAGGTGTCTGGGACATGCGCGGGAAACAGTTCTACAATGGGA
  TTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAGAAACAGTGTCGAGAAGAGGTGCTCAAGAA
  CTTCACAGACCAGCTTCGGAAGATTTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCATGCTTCTGC
  AAATACGCACAGGGGGCAGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAACACCTACTCAGGACTGC
  AGCTCATTATCGTCATCCTGCCCGGGAAGACGCCAGTGTATGCTGAAGTGAAACGTGTCGGAGATACACT
  CTTGGGAATGGCAACACAGTGTGTGCAGGTGAAGAATGTGGTCAAGACGTCACCGCAGACTCTGTCCAAC
  CTCTGCCTCAAGATCAATGTCAAACTCGGTGGCATTAACAACATCCTAGTCCCACACCAGCGCTCTGCTG
  TTTTTCAACAGCCAGTGATTTTCCTGGGAGCAGATGTTACACATCCCCCAGCTGGGGATGGGAAGAAACC
  ATCTATCACAGCAGTGGTAGGCAGCATGGACGCACACCCCAGCCGATACTGTGCCACCGTGCGTGTGCAG
  CGCCCACGGCAGGAGATCATTGAAGACTTGTCCTATATGGTGCGCGAGCTGCTTATCCAGTTCTACAAGT
  CCACCCGCTTCAAGCCCACCCGCATCATCTTCTACCGCGACGGCGTCCCCGAGGGCCAGCTGCCCCAGAT
  TCTTCACTATGAGCTGCTTGCCATTCGAGATGCATGCATCAAACTGGAAAAAGACTACCAGCCTGGGATC
  ACATATATTGTGGTGCAGAAGCGCCATCACACCCGCCTGTTCTGTGCTGACAAGAATGAGCGGATTGGGA
  AGAGTGGTAACATCCCAGCAGGGACCACTGTGGACACCAACATCACCCACCCATTTGAGTTCGACTTCTA
  TCTGTGCAGCCATGCAGGCATCCAGGGTACCAGCCGACCATCCCATTATTATGTCCTTTGGGATGACAAC
  CGTTTCACAGCAGATGAACTCCAGATCTTGACATACCAGCTGTGCCACACTTACGTACGATGCACACGTT
  CTGTCTCTATCCCAGCACCTGCCTACTATGCTCGCTTGGTGGCTTTCCGGGCACGATACCACCTGGTGGA
  CAAGGAACATGACAGTGGAGAGGGGAGCCACATATCAGGGCAAAGCAATGGGCGAGACCCCCAGGCCCTG
  GCCAAAGCTGTGCAGGTTCACCAGGATACTCTACGCACCATGTACTTCGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153403
• Insert Size: 2574 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153403.3, NP_700452.2
• RefSeq Size: 7255 bp
• RefSeq ORF: 2574 bp
• Locus ID: 236511
• UniProt ID: Q8CJG1
• Cytogenetics: 4 D2.2
• Gene Summary: This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.