Disc1 (NM_174854) Mouse Untagged Clone

CAT#: MC222089

Disc1 (untagged) - Mouse disrupted in schizophrenia 1 (Disc1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Disc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222089 representing NM_174854
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGCGGGGGTCCCCGGGGCGCTCCGATCCACAGTCCGAGCCACGGCGCAGACAGTGGGCATGGCT
  TACCGCCTGCAGTAGCCCCTCAGAGGCGGCGGCTGACACGGAGACCAGGCTACATGAGAAGCACAGCGGG
  TTCTGGGATCGGGTTCCTCTCTCCAGCAGTGGGCATGCCACACCCGAGCTCAGCAGGGCTGACAGGCCAG
  CAGTCCCAACACTCACAGTCCAAGGCTGGACAGTGCGGACTTGACCCTGGGAGCCACTGCCAAGCCTCAC
  TGGTGGGCAAGCCTTTTCTCAAGAGCTCCCTTGTCCCTGCTGTGGCCTCTGAGGGCCACCTGCACCCAGC
  CCAGCGCTCTATGAGAAAAAGACCAGTGCACTTTGCGGTTCATTCCAAGAATGACAGCAGACAATCTGAG
  AGGCTGACTGGGTCATTTAAGCCTGGGGACAGTGGGTTTTGGCAAGAATTATTATCTTCAGACAGCTTTA
  AGTCTCTGGCTCCTAGCCTTGATGCACCCTGGAACAAGGGATCAAGGGGCCTGAAGACTGTGAAACCTCT
  GGCATCACCGGCGTTGAATGGCCCCGCTGATATCGCATCCCTTCCCGGCTTCCAAGACACCTTCACTTCC
  AGCTTCAGCTTCATCCAACTCTCCCTTGGTGCTGCTGGAGAACGCGGAGAAGCAGAAGGTTGCCTGCCAT
  CCAGAGAGGCCGAACCTCTGCATCAGAGGCCCCAAGAGATGGCAGCTGAAGCATCTAGCTCAGACAGGCC
  CCACGGGGATCCTCGGCATCTCTGGACCTTCAGTCTTCACGCTGCTCCAGGCTTGGCGGACTTGGCTCAG
  GTGACAAGGAGCAGCAGCAGGCAATCAGAATGTGGCACGGTCTCCTCCTCCTCCTCGGACACTGGCTTCT
  CTTCCCAGGATGCATCCTCCGCTGGTGGGCGGGGCGACCAGGGCGGCGGCTGGGCCGATGCCCATGGATG
  GCATACATTGCTCAGGGAATGGGAGCCCATGCTGCAGGACTACCTACTGAGCAACCGCAGGCAGCTGGAG
  GTCACTTCCTTAATTTTAAAGCTTCAGAAATGTCAAGAAAAAGTGGTCGAGGATGGCGATTACGATACTG
  CAGAGACATTGAGACAGAGGTTGGAAGAACTGGAACAGGAGAAAGGCCGCCTGTCCTGGGCTCTGCCTTC
  ACAGCAACCTGCTCTTCGCAGCTTCTTGGGTTACCTGGCAGCACAGATACAGGTGGCCTTGCATGGAGCC
  ACCCAAAGGGCCGGCAGCGATGATCCAGAAGCCCCACTTGAAGGACAGCTGAGGACTACCGCCCAGGATA
  GCCTGCCTGCATCCATCACCAGGAGGGACTGGCTTATTCGAGAGAAACAGCGATTGCAGAAGGAAATCGA
  AGCTCTCCAAGCACGGATGTCTGCGCTGGAGGCAAAGGAAAAACGGCTGAGCCAAGAGTTGGAGGAGCAG
  GAGGTGCTGCTCCGGTGGCCGGGCTGTGACCTGATGGCACTGGTGGCCCAGATGTCCCCAGGCCAGCTGC
  AGGAGGTCAGCAAGGCCTTGGGAGAGACCCTGACCTCTGCCAACCAGGCTCCCTTCCAGGTGGAGCCACC
  TGAGACCCTCAGGAGCCTCCGGGAAAGGACAAAATCACTGAACCTGGCTGTCAGAGAACTCACTGCTCAG
  GTGTGCTCAGGTGAGAAGCTGTGCAGCTCTCTGAGGAGGAGACTCAGTGACCTCGACACCAGGCTGCCTG
  CCTTGCTGGAAGCCAAGATGCTGGCCCTATCAGGAAGCTGCTTCTCCACAGCCAAGGAGCTCACGGAGGA
  GATTTGGGCCTTGTCGTCAGAGCGGGAAGGGCTAGAGATGTTCCTGGGCAGGCTGTTGGCACTCAGCTCC
  AGGAACAGCAGAAGGCTGGGCATCGTCAAAGAGGATCACCTCAGGTGCAGGCAGGACCTGGCACTCCAGG
  ACGCCGCCCACAAAACACGCATGAAGGCAAACACTGTGAAGTGCATGGAAGTGTTGGAAGGTCAGCTGAG
  CAGCTGCAGGTGCCCGCTGCTTGGGAGAGTGTGGAAAGCAGACTTGGAGACTTGTCAGTTGCTAATGCAG
  AGCCTGCAGCTTCAGGAAGCAGGCAGCAGCCCACACGCAGAGGACGAGGAGCAGGTGCATAGCACAGGAG
  AGGCCGCCCAGACAGCTGCTCTGGCTGTCCCTCGAACACCCCACCCCGAAGAAGAAAAGTCCCCCTTGCA
  GGTGCTCCAGGAGTGGGACACCCACTCAGCTCTTTCACCACACTGTGCTGCAGGCCCATGGAAAGAGGAT
  TCTCACATCGTTTCTGCTGAAGTTGGAGAAAAGTGCGAAGCCATAGGCGTGAAGCTCCTACACCTGGAAG
  ACCAGCTTCTCGGAGCCATGTACAGTCACGATGAGGCTCTCTTTCAGTCTCTCCAGGGGGAGCTCCAGAC
  GGTGAAGGAAACACTGCAGGCCATGATCCTGCAGCTCCAGCCAACAAAGGAGGCAGGAGAGGCCTCAGCT
  TCCTATCCGACAGCTGGTGCTCAGGAAACCGAGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_174854
• Insert Size: 2559 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174854.2, NP_777279.2
• RefSeq Size: 2597 bp
• RefSeq ORF: 2559 bp
• Locus ID: 244667
• UniProt ID: Q811T9
• Cytogenetics: 8 73.26 cM
• Gene Summary: Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) is the longer transcript and it encodes the longer protein (isoform 1).