Nefm (NM_008691) Mouse Untagged Clone

CAT#: MC222041

Nefm (untagged) - Mouse neurofilament, medium polypeptide (Nefm), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nefm
• Synonyms: Nef3; NF-M; NF160; NF165; Nfm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222041 representing NM_008691
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACACGCTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGGGTCACCGAGACCCGCTCCAGCT
  TCAGCCGCGTGAGCGGTTCCCCGTCCAGCGGCTTCCGCTCGCAGTCCTGGTCCCGCGGCTCGCCCAGCAC
  CGTGTCCTCCTCCTACAAGCGCAGCGCGCTCGCCCCGCGTCTCGCCTACAGCTCGGCTATGCTCAGCTCG
  GCCGAGAGCAGCCTCGACTTCAGCCAGTCCTCGTCGCTGCTCAACGGCGGCTCCGGCGGCGACTACAAAC
  TGTCCCGCTCTAACGAGAAAGAGCAGCTGCAGGGGCTGAACGACCGCTTCGCCGGCTACATCGAGAAAGT
  GCACTACTTGGAACAACAGAACAAGGAGATCGAAGCAGAGATCCAGGCACTGCGGCAGAAGCAGGCCTCG
  CACGCCCAGCTGGGTGATGCTTACGACCAGGAGATCCGAGAGCTGCGCGCCACCCTCGAGATGGTGAACC
  ACGAGAAGGCTCAAGTGCAGCTGGACTCCGATCACTTGGAGGAAGACATCCACCGGCTCAAGGAGCGCTT
  CGAGGAGGAGGCGCGGCTGCGGGACGACACCGAGGCTGCCATTCGCGCGCTGCGCAAAGACATCGAAGAG
  TCGTCGATGGTTAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCTTTCCTGCGGA
  GCAATCACGAAGAGGAGGTGGCCGACCTGCTGGCTCAGATCCAGGCGTCGCACATCACGGTAGAGCGCAA
  AGATTACCTGAAGACAGACATCTCCACGGCGCTGAAGGAGATCCGCTCCCAGCTCGAGTGTCACTCAGAC
  CAGAACATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCGAGCAGA
  ACAAGGAGGCCATTCGCTCTGCCAAGGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAAGAGCAT
  CGAGCTCGAGTCGGTGCGAGGCACTAAGGAGTCCCTGGAGCGGCAGCTCAGCGACATCGAGGAGCGCCAC
  AACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGTTGGAAAATGAACTTCGGGGAACCAAGTGGG
  AAATGGCTCGTCATTTGCGAGAATACCAGGATCTCCTTAACGTCAAGATGGCCCTGGACATCGAGATCGC
  CGCGTACAGGAAACTCCTAGAGGGGGAAGAGACCAGATTTAGCACATTTTCAGGAAGCATCACCGGGCCT
  CTGTACACACACCGACAGCCCTCAGTCACAATATCCAGTAAGATTCAGAAGACCAAAGTCGAGGCCCCCA
  AGCTCAAGGTCCAACACAAATTTGTGGAGGAGATCATCGAAGAAACTAAAGTGGAAGATGAGAAGTCAGA
  AATGGAAGAAACCCTCACAGCCATCGCAGAGGAGTTGGCAGCCTCCGCCAAAGAGGAGAAGGAAGAGGCC
  GAAGAAAAGGAGGAGGAACCAGAAGCCGAAAAGTCTCCCGTGAAGTCTCCTGAGGCTAAGGAAGAGGAGG
  AGGAAGGGGAAAAGGAGGAAGAAGAGGAAGGCCAGGAGGAAGAAGAGGAGGAAGATGAAGGTGTCAAGTC
  AGACCAGGCAGAAGAGGGGGGATCTGAGAAGGAAGGCTCCAGTGAAAAAGATGAAGGTGAGCAGGAAGAA
  GAAGAAGGAGAAACCGAGGCAGAAGGTGAAGGAGAGGAAGCAGAAGCTAAGGAGGAAAAGAAAATTGAGG
  GAAAGGTTGAGGAAGTGGCTGTCAAGGAGGAAATCAAGGTCGAGAAGCCTGAGAAAGCCAAATCCCCTAT
  GCCCAAATCACCCGTGGAAGAAGTAAAGCCAAAACCAGAGGCCAAGGCCGGGAAGGGTGAGCACAAGGAG
  GAAGAGAAAGTTGAGGAAGAGAAGAAGGAAGTCACCAAAGAATCACCCAAGGAAGAGAAGGTGGAGAAAA
  AGGAGGAGAAGCCAAAAGATGTTGCAGATAAAAAGAAGGCCGAGTCCCCGGTGAAAGAGAAGGCTGTGGA
  GGAGGTGATCACCATCAGCAAGTCGGTAAAGGTGAGCCTAGAGAAAGACACCAAAGAGGAGAAGCTGCAG
  CCGCAGGAGAAGGTGAAGGAGAAGGCAGAGGAGGAGGGGGGCAGTGAGGAGGAAGGGAGTGACCGTAGCC
  CGCAGGAGTCCAAGAAGGAAGACATAGCTATCAATGGGGAGGTGGAAGGAAAAGAGGAGGAGGAGCAGGA
  AACTCAGGAGAAGGGCAGTGGGCGGGAGGAGGAGAAAGGGGTGGTCACTAATGGCTTAGATGTGAGCCCT
  GCAGAGGAGAAGAAAGGAGAGGATAGCAGTGATGATAAAGTGGTGGTCACCAAGAAGGTAGAAAAGATCA
  CCAGCGAGGGGGGCGATGGTGCTACCAAATACATCACCAAATCTGTAACCGTCACTCAAAAGGTTGAAGA
  GCATGAGGAGACCTTTGAGGAGAAGCTGGTCTCAACTAAAAAGGTAGAAAAGGTCACTTCACACGCCATA
  GTCAAGGAAGTCACCCAGGGTGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008691
• Insert Size: 2547 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008691.2, NP_032717.2
• RefSeq Size: 3244 bp
• RefSeq ORF: 2547 bp
• Locus ID: 18040
• UniProt ID: P08553
• Cytogenetics: 14 D1
• Gene Summary: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.[UniProtKB/Swiss-Prot Function]