Abcf1 (NM_013854) Mouse Untagged Clone

CAT#: MC222022

Abcf1 (untagged) - Mouse ATP-binding cassette, sub-family F (GCN20), member 1 (Abcf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Abcf1
• Synonyms: Abc50; AU041969; D17Wsu166e; GCN20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222022 representing NM_013854
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAAGGGTCCCAAGCAACAGCCGCCCGAGCCCGAGTGGATCGGGGACGGCGAGGGCACGAGCCCCG
  CGGACAAAGTAGTGAAGAAAGGAAAAAAGGACAAGAAGACCAAAAAGACGTTCTTTGAAGAGCTGGCAGT
  AGAGGACAAACAAGCTGGGGAAGAGGAAAAATTGCAGAAGGAGAAGGAGCAGCAACAGCAACAGCAGCAA
  CAGAAGAAAAAGCGAGACACCAGGAAAGGCCGTCGGAAAAAGGATGTGGACGATGATAGTGATGAGAGAG
  TGCTCATGGAGCGACTTAAGCAACTGTCTGTGCCAGCCAGTGATGAGGAAGATGAGGTGCCTGCCCCCAT
  ACCCCGAGGACGGAAGAAGGCCAAGGGTGGAAATGTTTTTGAAGCCCTGATTCAGGATGACAGTGAGGAG
  GAGGAAGAGGAGGAAGAAAACCGTGTTCTCAAGCCCGCCAAGCCAGAGAAGAATCGCATCAATAAAGCCG
  TGGCTGAGGAACCTCCTGGGCTCAGGAGTAAAAAGGGAAAGGAGGAGAAATCAAAAGGGAAAGCCAAGAG
  TAAACCTGCTGCTGCAGACAGTGAAGGGGAAGAGGAGGAGGAGGACACAGCTAAAGAAAAGGAGCCTCCT
  CAGCAAGGGAAGGACAGAGACAAAAAGGAGGCTGAGCAGGGCTCAGGGGAAGAGAAGGAAGAGAAAGAAG
  GGGACTTGAAGGCAAACGATCCCTATGCCAACCTTAGCAAAAAGGAAAAGAAAAAGCTAAAGAAACAGAT
  GGATTATGAACGACAGGTGGAGTCATTGAAAGCAGCTAATGCTGCAGAAAACGACTTCTCTGTGTCCCAG
  GCAGAGGTGTCTTCCCGCCAGGCAATGTTAGAAAATGCATCTGACATTAAGTTGGAAAAGTTCAGCATCT
  CCGCCCACGGCAAGGAGCTATTCGTCAATGCTGACCTGTACATAGTAGCCGGCCGCCGCTATGGGCTGGT
  GGGACCCAACGGCAAAGGCAAAACCACGCTTCTGAAGCACATTGCCAACCGTGCCCTGAGCATCCCCCCT
  AACATTGACGTGCTGCTGTGCGAGCAGGAGGTGGTGGCTGATGAAACACCAGCCGTGCAAGCTGTCCTTC
  GAGCAGATACCAAGCGACTGAGGTTGCTAGAGGAGGAGAGACGGCTTCAGGGACAGCTGGAGCAGGGGGA
  TGACACTGCTGCTGAGAAACTAGAAAAGGTGTATGAGGAACTGCGAGCTACCGGGGCAGCAGCTGCAGAG
  GCCAAGGCACGGCGGATCCTGGCTGGCTTGGGCTTCGACCCTGAGATGCAGAATCGGCCCACACAGAAGT
  TCTCTGGGGGTTGGAGAATGCGTGTCTCCCTGGCCAGGGCACTGTTCATGGAGCCAACGCTGCTGATGTT
  GGATGAGCCCACTAACCACCTGGACCTCAACGCCGTCATCTGGCTCAATAACTACCTTCAGGGCTGGAGG
  AAGACGTTGCTGATTGTCTCCCACGACCAGGGCTTTCTGGATGACGTTTGCACTGATATCATCCACCTGG
  ACACCCAGCGGCTCCATTACTACAGGGGCAATTACATGACCTTCAAGAAGATGTACCAGCAGAAGCAGAA
  AGAGCTGCTAAAGCAGTACGAGAAGCAGGAGAAGAAACTGAAGGAGCTGAAGGCTGGGGGCAAGTCCACC
  AAGCAAGCGGAAAAGCAAACAAAGGAAGTCCTGACTCGAAAACAGCAGAAGTGCCGACGGAAAAACCAGG
  ATGAAGAGTCTCAGGAGCCCCCTGAGCTCCTGAAGCGTCCCAAGGAGTACACCGTGCGCTTCACCTTCCC
  AGACCCCCCGCCTCTCAGCCCACCTGTGCTGGGCCTGCACGGTGTGACGTTTGGCTACGAGGGGCAGAAG
  CCACTCTTTAAGAATCTAGATTTCGGCATCGACATGGACTCCCGGATTTGCATCGTGGGTCCCAATGGTG
  TGGGGAAGAGCACACTACTCCTGCTGCTGACTGGCAAGCTGACACCGACCAACGGGGAGATGAGGAAGAA
  CCATCGGCTGAAAATCGGCTTCTTTAACCAGCAGTACGCAGAGCAGCTGCACATGGAGGAGACGCCCACT
  GAGTACCTGCAGCGGAGCTTCAATCTGCCCTACCAGGATGCCCGGAAGTGCTTGGGCCGCTTTGGCCTGG
  AGAGCCACGCCCACACCATCCAGATCTGCAAACTCTCCGGTGGGCAGAAAGCCCGAGTTGTGTTTGCGGA
  GCTGGCCTGTCGGGAGCCTGATGTCCTCATCTTGGATGAACCAACCAATAACTTGGACATAGAGTCCATC
  GATGCCCTGGGGGAGGCCATCAACGACTACAAGGGGGCTGTGATCGTTGTCAGCCACGATGCGCGCCTCA
  TCACAGAAACCAACTGCCAGTTGTGGGTGGTGGAGGAGCAGGGTGTCAGTCAGATCGACGGCGACTTTGA
  TGACTACAAGCGAGAGGTGTTGGAGGCCCTGGGTGAGGTCATGGTCAACCGTCCTCGGGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013854
• Insert Size: 2514 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013854.1, NP_038882.1
• RefSeq Size: 3207 bp
• RefSeq ORF: 2514 bp
• Locus ID: 224742
• UniProt ID: Q6P542
• Cytogenetics: 17 18.8 cM
• Gene Summary: Required for efficient Cap- and IRES-mediated mRNA translation initiation. Not involved in the ribosome biogenesis (By similarity).[UniProtKB/Swiss-Prot Function]