Pan3 (NM_028291) Mouse Untagged Clone

CAT#: MC222008

Pan3 (untagged) - Mouse PAN3 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pan3
• Synonyms: 2700050F09Rik; A430027N15Rik; AU014670
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222008 representing NM_028291
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGTGGCGGCGGCGGTGGCCTCCCGCCGCCCTCGGCCGCTGCCTCGCCGTCGTCGTCCTCGCTGG
  CGGCGGCCGTGGCGGTGGCGGTGGCGGCCTCGTCGGGCGTCGGGGGCGTCCCCGGCGGGCCGGCGGCGGC
  GGCGGGCGTGAAGCTCAAGTACTGCCGCTACTACGCCAAGGACAAGACGTGCTTCTACGGCGAGGAGTGT
  CAGTTCCTGCACGAGGACCCAGCCGCCGGCGCGGCCCCGGGGCTTGGCCTCCATAGCAACAGCGTCCCGC
  TGGCGCTAGCGGCCGCGGCCGGGGCCGCCTTTCCTCCGGGAGCGCTCCCGGGCGGGGGAGCGGGGCCGCC
  CGCGGGGCCCAAGAAGCCGGAGCTGGGCGTCCCGGGGGCGGCGACCGCGGGCGGCGGACTCGATGGCCCG
  CGGGTGGCGATTCCGGGAATGGATGGAGGTGCTTTAACTGATGCGAGTCTCACAGAGTCCTATTTCAGCA
  CCAGCTTTATTGGAGTCAATGGATTTGGAAGCCCTGTAGAAACAAAGTATCCCTTGATGCAGAGAATGAC
  TAGTAGTAGCAGCTCCCCAAGCCTTCTAAATGACAGTGCCAAGCCATACACAGGCCACGATCTTCTGACT
  TCATCTGCTTCATCCTTGTTTAATGACTTCGGTGCCCTCAACATCTCCCAGAGAAGGAAGACACCAAATC
  CTACTGCAAGCGAATTTATACCTAAAGGAGGATCAACCTCCAGGCTGAGTAACGTGTCCCAGTCAAATAT
  GTCTGCCTTCTCTCAAGTTTTCTCTCACCCATCCATGGGAAGTCCTGCTACTGCTGGATTAGCACCAGGA
  ATGTCACTGTCTGCTGGATCTTCCCCTCTCCATTCCCCCAAAATTACTCCACACACCTCTCCTGCTCCTA
  GAAGAAGAAGCCACACTCCAAACCCAGCAAGTTTCATGGTGCCGCCTAGTGCCTCCACTCCTGCCAATAA
  CCCTGCTCCTCAGCCTCCGTCCTCTGGTCAGGTGATCCAGAAGGAGACTGTCGGGGGGACGACTTACTTC
  TATACAGATACAACCCCAGCACCTTTGACTGGAATGGTATTTCCAAACTATCATATTTATCCTCCAACCG
  CACCTCATGTTGCTTATATGCAACCAAAAGCAAATGCACCTTCCTTCTTCATGGCCGACGAGCTCCGACA
  GGAGCTGATCAACAGACATTTAATAACGATGGCTCAAATTGACCAAGCAGATATGCCAGCAGTCCCCACA
  GAAGTTGACAGCTACCATAGCCTGTTCCCTCTAGAACCACTGCCACCTCCCAACCGGATACAGAAATCAA
  GTAATTTTGGATATATCACGTCTTGCTACAAAGCTGTAAATAGCAAAGATGATCTGCCATATTGCCTTCG
  GAGGATACATGGTTTTCGTCTTGTTAACACAAAGTGCATGGTGTTGGTTGATATGTGGAAAAAAATTCAG
  CACTCAAATATTGTAACTCTGCGTGAAGTGTTTACCACTAAAGCATTTGCCGAGCCTTCTCTGGTGTTCG
  CCTATGATTTCCATGCTGGAGGAGAAACTATGATGAGCAGACACTTTAACGACCCGAATTCTGATGCCTA
  CTTCACAAAGAGGAAGTGGGGCCAGCACGATGGACCACTGCCCAGGCAGCATGCTGGATTGTTACCAGAG
  TCTCTCATCTGGGCATACATTGTACAGCTGAGTTCTGCTCTGCGGACCATTCATACAGCAGGTTTGGCCT
  GTAGAGTAATGGACCCAACGAAGATTCTGATAACCAGCAAAACAAGGTTACGAGTAAATTGTGTAGGAGT
  CTTTGATGTTTTAACATTTGATAACAGTCAAAATAATAATCCTTTGGCATTAATGGCTCAGTACCAGCAA
  GCAGATCTGATATCATTAGGAAAAGTTGTGTTGGCTTTGGCTTGCAACTCTTTGGCAGGAATTCAGCGAG
  AAAATTTACAGAAAGCCATGGAATTGGTGACAATCAACTACTCCTCTGACCTGAAGAACCTCATCTTGTA
  CTTGTTGACTGACCAAAACAGGATGCGGAGTGTGAATGACATCATGCCCATGATTGGTGCTCGGTTTTAC
  ACTCAATTGGATGCTGCTCAAATGAGAAATGATGTTATCGAGGAAGACCTTGCTAAGGAGGTTCAAAATG
  GAAGACTGTTTAGGCTTCTAGCAAAATTGGGAACAATCAACGAGAGGCCGGAGTTTCAGAAGGATCCAAC
  CTGGTCAGAGACTGGAGACAGATACCTGTTGAAACTCTTTAGAGATCATCTTTTTCATCAGGTGACAGAA
  GCAGGTGCTCCCTGGATCGACCTCAGTCACATCATTTCTTGTCTTAACAAGCTAGATGCTGGTGTGCCAG
  AAAAAATCAGCCTGATTTCCAGAGACGAGAAGAGCGTGCTGGTGGTGACATACAGTGACCTAAAACGCTG
  CTTTGAAAATACCTTTCAAGAACTGATTGCGGCTGCAAATGGTAACGATCGGAACAGTAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028291
• Insert Size: 2514 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028291.4, NP_082567.3
• RefSeq Size: 4628 bp
• RefSeq ORF: 2514 bp
• Locus ID: 72587
• UniProt ID: Q640Q5
• Cytogenetics: 5 G3
• Gene Summary: Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data. The upstream in-frame AUG is conserved in all mammals with available genomic sequences in this region. and results in a 146aa-longer protein, but the splice sites are unaffected.