Elfn1 (NM_175522) Mouse Untagged Clone

CAT#: MC221902

Elfn1 (untagged) - Mouse leucine rich repeat and fibronectin type III, extracellular 1 (Elfn1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Elfn1
• Synonyms: A930017N06Rik; AI197272; Ppp1r28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221902 representing NM_175522
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGCACGGGTGGGGTACAGCGTGGGTACTTGTGGCAGCCGCCACGTTACTGCACGCGGGTGGGC
  TGGCCCAAGGTGACTGCTGGCTGATTGAGGGTGACAAAGGCTTCGTGTGGTTGGCCATCTGCAGCCAGAA
  CCAGCCACCCTATGAGGCCATCCCCCAACAGATCAACAACACCATCGTGGACCTGCGGCTTAACGAGAAC
  CGTATCCGCAGCGTGCAGTACGCCTCCCTGAGCCGCTTCGGAAACCTCACATACCTCAACCTCACCAAGA
  ACGAGATCGGCTACATCGAGGACGGGGCCTTCTCCGGCCAGTTCAACCTCCAGGTGCTGCAGCTGGGCTA
  CAACCGGCTGCGCAACCTCACGGAGGGCATGCTGCGGGGCCTGAGCAAGCTCGAGTACCTGTACCTACAG
  GCCAACCTCATCGAGGTGGTGATGGCCAGCGCCTTCTGGGAGTGTCCCAACATCGTCAACATAGATCTCT
  CCATGAATCGCATCCAGCAGCTGGGCAGCGGCACCTTCGCAGGCCTGACCAAGCTCTCGGTGTGTGAGAT
  CTACAGCAACCCTTTCTACTGCTCCTGTGAGCTGCTGGGTTTCCTGCGCTGGCTGGCTGCCTTCACCAAC
  GCCACACAGACCCATGACCGGGTGCAGTGTGAGTCTCCTCCTGTCTACGCAGGCTACTTCCTCCTGGGCC
  AGGGCCGCCACGGCCACCAACGTAGCATCCTTAGCAAGCTTCAGTCTGTGTGCACAGAGGGCTCCTACAC
  TGCTGAGGTGCTTGGCCCACCCCGTCCGGTGCCAGGCCGGTCACAGCCGGGACATTCGCCACCTCCGCCT
  CCACCAGAGCCTAGTGACATGCCCTGCGCTGATGACGAGTGCTTCTCCGGAGATGGCACCACGCCGCTGG
  TGATCCTGACTACGCTGGTCCCTCAGACAGAGGCCCGTCCCTCCATGAAGGTCAAGCAGCTGACCCAAAA
  TTCGGCCACCATCATGGTGCAGCTGCCGAGCCCGTTCAACCGCATGTACACGCTGGAGCAGTACAATAAC
  AGCAAATCGTTCACCGTGTCCAAGCTGACCCAGCCTCAGGAGGAGATCCGCCTCACCAATCTTTACACAC
  TCACCAACTACACATACTGCGTGGTCTCTACCAGCTCCGGCACCCATCATAACCACACCTGCCTTACCAT
  CTGCCTGCCCAAGCCGCCCAGCCCACCGGGCCCCGTGCCCAGCCCCTCCACAGCCACCCACTACATCATG
  ACCATCCTGGGCTGCCTCTTTGGTATGGTACTGGTTCTGGGCGCTGTCTATTACTGCCTGCGCAAGCGGA
  GGCGACAAGAGGAGAAACACAAGAAGGCTGTGGCAGCGGCAGCTGGCAGCTTGAAAAAAACCATCATCGA
  GCTTAAGTATGGTCCTGAGATTGAGGCGCCTGGCCTGGCTCCCCTGACCCAGGGTCCGCTGCTTGGCCCT
  GAGGCTGTGACTCGAATACCCTACCTGCCAGCCGCCACCAGTGACGTGGAGCAGTATAAGCTGGTGGAGA
  GCAGCGAGACCCCCAAGGCCACCAAGGGGAACTACATTGAAGTGCGCACGGGGGAGCCGCAGGAGCGCAG
  AGGCTGTGAGCTGAGCCGGCCAGGTGAACCCCAGAGCTCAGTAGCGGAGATCTCCACCATTGCCAAGGAA
  GTGGACAGAGTCAACCAGATCATTAACAACTGCATCGACGCCCTCAAGTCCGAGTCCACCTCCTTCCAGG
  GCGCCAAATCAGGGGCCGTGTCTGCGGCTGAGCCGCAGCTGGTGCTGCTGTCCGAGCCTCTGGCCAGCAA
  GCACAGCTTCCTGTCCCCTGTATACAAAGATGCCTTTGGCCATGGTGGCCTGCAGCGGCACCACAGTGTG
  GAGGCTGCGCCCGGGCCCCCGAGGGCCAGCACTTCATCCAGCGGTTCTGCACGCAGCCCTCGCACCTTCC
  GGGCTGAGGCCACCGGCACACATAAGGCCCCAGCCACCGAGACCAAGTACATTGAAAAAAGCTCACCTGT
  GCCCGAGACCATCCTCACTGTGACACCCGCGGCCACTGTGCTGCGGGCGGAGGCCGACAAGAGTCGCCAG
  TACGGCGAGCATCGGCACTCATACCCCGGCTCTCACCCTGCCGAGCCACCTGCCCCTCCACCACCTCCAC
  CCACTCACGAGGGCTTGGGTGGCCGCAAGGCATCCATCCTGGAGCCTCTGACCCGGCCACGACCCCGAGA
  CCTCGTCTACTCGCAGCTGTCCCCACAGTACCACAACCTGAGCTATTCATCCAGCCCCGAGTACACCTGC
  AGGGCATCCCCAAGCATCTGGGAGCGTCTCAGACTGAGCCGACGGAGACACAAGGATGATGCGGAGTTCA
  TGGCGGCTGGCCACGCCCTGCGCAAGAAGGTTCAGTTTGCCAAAGACGAGGACCTGCATGACATCCTAGA
  CTACTGGAAAGGCGTGTCTGCCCAGCACAAGTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175522
• Insert Size: 2487 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175522.3, NP_780731.1
• RefSeq Size: 3835 bp
• RefSeq ORF: 2487 bp
• Locus ID: 243312
• UniProt ID: Q8C8T7
• Cytogenetics: 5 G2
• Gene Summary: Postsynaptic protein that regulates circuit dynamics in the central nervous system by modulating the temporal dynamics of interneuron recruitment. Specifically present in excitatory synapses onto oriens-lacunosum molecular (OLM) interneurons and acts as a regulator of presynaptic release probability to direct the formation of highly facilitating pyramidal-OLM synapses. Inhibits phosphatase activity of protein phosphatase 1 (PP1) complexes.[UniProtKB/Swiss-Prot Function]