Cdc27 (NM_145436) Mouse Untagged Clone

CAT#: MC221891

Cdc27 (untagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdc27
• Synonyms: AI452358; APC3; BC023187
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221891 representing NM_145436
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTGCTGCAGGAACCTGTCCAGGCTGCTATATGGCAAGCGCTAAACCACTATGCTTACCGAGATG
  CAGTTTTCCTCGCAGAACGACTATATGCAGAAGTACATTCAGAAGAAGCCTTGTTTTTACTGGCAACCTG
  TTACTACCGCTCAGGAAAGGCTTATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACCACCCCACAG
  TGTAAATACCTGCTTGCAAAATGTTGTGTTGACCTCAGCAAGCTTGCAGAAGGGGAACAGATCTTATCTG
  GTGGAGTGTTTAATAAGCAGAAAAGCCATGACGACCTTGTCACTGAGTTTGGAGATTCAGCTTGCTTCAC
  TCTTTCCTTGTTGGGACATGTGTATTGCAAGACAGATCGGCTTGCCAAAGGGTCAGAATGTTACCAAAAG
  AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCGTTATGTGAAATAGGTGAGAAGCCAGATC
  CTGACCAAACATTTAAATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAACACTTGTACAACTCT
  AGTATCTAATCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACACCCCAAGACACAATT
  GAATTAAACAGACTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCCTCCGTGTCTT
  ACATTGATTCAACTGTAATTTCACCAGATAACGTCCCCCTTGGACCTGGTACTGCCATATTATCTAAACA
  GGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAACTGCTCTTAGCCCATTAACCCCA
  AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATA
  CACCTTCTGTAATTGATGTGGCACCCACCGGAGCACCTACAAAAAAGTCTGTTGCAAGAATGGGCCAAAC
  TGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGTCGAGAGGTCACACCAGTACTTGTTGCACAAACA
  CAAAGTTCTGGCCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATCACATCTCCCCCAAACG
  CATTGCCTCGGAGAAGTTCCCGCCTTTTCACTAGTGACAGTTCTACAACCAAGGAGAATAGCAAAAAGTT
  AAAAATGAAGTTTCCACCTAAAATCCCTAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGACTAACT
  CAGCCCAGCATAAACGATAGTCTGGAAATTACAAAACTGGACTCCTCTATCATTTCAGAAGGGAAAATAA
  CCACAGTCACACCTCAGATCCAGGCATTTAACCTACAAAAGGCAGCAGCAGAAGGTTTGATGAGCCTTCT
  TCGTGAAATGGGAAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCTATCAATATTTTGAGC
  CATCTACCTTCTCATCACTACAGTACTGGTTGGGTCCTATGCCAGATTGGACGGGCTTATTTTGAACTTT
  CAGAATACATGCAGGCTGAAAGAATATTCTCAGAGGTTAGAAGGATTGAGAGTTTCAGAGTTGAAGGAAT
  GGAGATCTACTCTACAACACTCTGGCATCTTCAGAAAGACGTTGCTCTTTCAGTTCTTTCAAAAGATTTA
  ACAGACATGGATAAGAATTCACCAGAGGCCTGGTGTGCTGCAGGGAATTGTTTCAGTCTACAACGAGAGC
  ATGATATAGCAATTAAATTCTTCCAAAGAGCTATCCAGGTCGATCCAAATTACGCTTACGCCTATACTCT
  ATTAGGACATGAGTTTGTGTTAACTGAAGAACTAGATAAAGCATTAGCATGTTTTCGAAATGCTATAAGA
  GTAAATCCCAGACATTACAATGCATGGTATGGTTTAGGAATGATTTATTACAAGCAAGAGAAGTTCAGCC
  TCGCAGAAATGCATTTCCAGAAAGCACTTGATATCAACCCTCAGAGTTCAGTTTTACTTTGCCACATTGG
  AGTAGTTCAGCATGCACTAAAGAAGTCTGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTTATCGAT
  CCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCGGTTTTATTTGCAAATGAAAAATACAAGTCTGCTT
  TACAAGAACTCGAGGAACTGAAGCAGATTGTCCCCAAAGAGTCCCTCGTTTACTTCCTAATAGGAAAGGT
  TTACAAGAAGTTGGGTCAAACTCATCTCGCCTTGATGAATTTCTCTTGGGCTATGGATTTAGATCCTAAA
  GGAGCCAATAATCAGATTAAAGAGGCAATTGACAAGCGCTACCTTCCAGATGATGAGGAGCCAATAACCC
  AGGAGGAACAGATCATGGGGACAGATGAATCCCAGGAGAGCAGCATGACAGATGCAGATGACACACAACT
  TCATGCGGCGGAAAGTGACGAATTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145436
• Insert Size: 2478 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145436.2, NP_663411.2
• RefSeq Size: 5783 bp
• RefSeq ORF: 2478 bp
• Locus ID: 217232
• UniProt ID: A2A6Q5
• Cytogenetics: 11 67.79 cM
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1.