Cdc27 (NM_145436) Mouse Tagged ORF Clone

CAT#: MG223434

Cdc27 (tGFP-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27), (10ug)

Product Images

Circular map for MG223434

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdc27
• Synonyms: AI452358; APC3; BC023187
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223434 representing NM_145436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTGCTGCAGGAACCTGTCCAGGCTGCTATATGGCAAGCGCTAAACCACTATGCTTACCGAGATG
  CAGTTTTCCTCGCAGAACGACTATATGCAGAAGTACATTCAGAAGAAGCCTTGTTTTTACTGGCAACCTG
  TTACTACCGCTCAGGAAAGGCTTATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACCACCCCACAG
  TGTAAATACCTGCTTGCAAAATGTTGTGTTGACCTCAGCAAGCTTGCAGAAGGGGAACAGATCTTATCTG
  GTGGAGTGTTTAATAAGCAGAAAAGCCATGACGACCTTGTCACTGAGTTTGGAGATTCAGCTTGCTTCAC
  TCTTTCCTTGTTGGGACATGTGTATTGCAAGACAGATCGGCTTGCCAAAGGGTCAGAATGTTACCAAAAG
  AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCGTTATGTGAAATAGGTGAGAAGCCAGATC
  CTGACCAAACATTTAAATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAACACTTGTACAACTCT
  AGTATCTAATCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACACCCCAAGACACAATT
  GAATTAAACAGACTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCCTCCGTGTCTT
  ACATTGATTCAACTGTAATTTCACCAGATAACGTCCCCCTTGGACCTGGTACTGCCATATTATCTAAACA
  GGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAACTGCTCTTAGCCCATTAACCCCA
  AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATA
  CACCTTCTGTAATTGATGTGGCACCCACCGGAGCACCTACAAAAAAGTCTGTTGCAAGAATGGGCCAAAC
  TGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGTCGAGAGGTCACACCAGTACTTGTTGCACAAACA
  CAAAGTTCTGGCCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATCACATCTCCCCCAAACG
  CATTGCCTCGGAGAAGTTCCCGCCTTTTCACTAGTGACAGTTCTACAACCAAGGAGAATAGCAAAAAGTT
  AAAAATGAAGTTTCCACCTAAAATCCCTAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGACTAACT
  CAGCCCAGCATAAACGATAGTCTGGAAATTACAAAACTGGACTCCTCTATCATTTCAGAAGGGAAAATAA
  CCACAGTCACACCTCAGATCCAGGCATTTAACCTACAAAAGGCAGCAGCAGAAGGTTTGATGAGCCTTCT
  TCGTGAAATGGGAAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCTATCAATATTTTGAGC
  CATCTACCTTCTCATCACTACAGTACTGGTTGGGTCCTATGCCAGATTGGACGGGCTTATTTTGAACTTT
  CAGAATACATGCAGGCTGAAAGAATATTCTCAGAGGTTAGAAGGATTGAGAGTTTCAGAGTTGAAGGAAT
  GGAGATCTACTCTACAACACTCTGGCATCTTCAGAAAGACGTTGCTCTTTCAGTTCTTTCAAAAGATTTA
  ACAGACATGGATAAGAATTCACCAGAGGCCTGGTGTGCTGCAGGGAATTGTTTCAGTCTACAACGAGAGC
  ATGATATAGCAATTAAATTCTTCCAAAGAGCTATCCAGGTCGATCCAAATTACGCTTACGCCTATACTCT
  ATTAGGACATGAGTTTGTGTTAACTGAAGAACTAGATAAAGCATTAGCATGTTTTCGAAATGCTATAAGA
  GTAAATCCCAGACATTACAATGCATGGTATGGTTTAGGAATGATTTATTACAAGCAAGAGAAGTTCAGCC
  TCGCAGAAATGCATTTCCAGAAAGCACTTGATATCAACCCTCAGAGTTCAGTTTTACTTTGCCACATTGG
  AGTAGTTCAGCATGCACTAAAGAAGTCTGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTTATCGAT
  CCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCGGTTTTATTTGCAAATGAAAAATACAAGTCTGCTT
  TACAAGAACTCGAGGAACTGAAGCAGATTGTCCCCAAAGAGTCCCTCGTTTACTTCCTAATAGGAAAGGT
  TTACAAGAAGTTGGGTCAAACTCATCTCGCCTTGATGAATTTCTCTTGGGCTATGGATTTAGATCCTAAA
  GGAGCCAATAATCAGATTAAAGAGGCAATTGACAAGCGCTACCTTCCAGATGATGAGGAGCCAATAACCC
  AGGAGGAACAGATCATGGGGACAGATGAATCCCAGGAGAGCAGCATGACAGATGCAGATGACACACAACT
  TCATGCGGCGGAAAGTGACGAATTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223434 representing NM_145436
  Red=Cloning site Green=Tags(s)
  
  MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQ
  CKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDLVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQK
  SLSLNPFLWSPFESLCEIGEKPDPDQTFKLTSLQNFSSCLPNTCTTLVSNHSLSHRQPETVLTETPQDTI
  ELNRLNLESSNSKYSLNTDSSVSYIDSTVISPDNVPLGPGTAILSKQVQNKPKTGRSLLGGPTALSPLTP
  SFGILPLETPSPGDGSYLQNYTNTPSVIDVAPTGAPTKKSVARMGQTGTKSVFSQSGNSREVTPVLVAQT
  QSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGLT
  QPSINDSLEITKLDSSIISEGKITTVTPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILS
  HLPSHHYSTGWVLCQIGRAYFELSEYMQAERIFSEVRRIESFRVEGMEIYSTTLWHLQKDVALSVLSKDL
  TDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIR
  VNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVID
  PKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPK
  GANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145436
• ORF Size: 2475 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145436.2, NP_663411.2
• RefSeq Size: 5783 bp
• RefSeq ORF: 2478 bp
• Locus ID: 217232
• UniProt ID: A2A6Q5
• Cytogenetics: 11 67.79 cM
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]