Map4k1 (NM_008279) Mouse Untagged Clone

CAT#: MC221868

Map4k1 (untagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 1 (Map4k1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map4k1
• Synonyms: Hpk1; mHPK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221868 representing NM_008279
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTTGTGGACCCCGACATTTTTAATAAGGACCCTCGAGAACACTATGATCTACTGCAACGACTGG
  GTGGTGGAACTTATGGAGAAGTATTCAAGGCTCGCGACAAGGTGTCTAAGGACCTGGTGGCTTTGAAGAT
  GGTGAAGATGGAGCCTGATGATGACGTCGCCACCCTTCAGAAGGAGATTCTCATGCTGAAAACTTGCCGG
  CACGCCAACATCGTGGCTTACCATGGCAGTTACCTCTGGCTGCAGAAGCTGTGGATCTGCATGGAATTCT
  GTGGAGCTGGTTCTCTCCAGGACATCTACCAAGTGACTGGCTCTCTGTCCGAGCTCCAGATCAGCTATGT
  GTGCCGGGAAGTGCTTCAGGGACTGGCCTACCTGCATTCAGAGAAGAAGATACATCGAGACATCAAGGGA
  GCCAACATCCTCATCAACGACTGCGGGGAGGTTAAGCTGGCTGACTTTGGGATCTCAGCCCAGATTGGGG
  CGACACTGGCCCGGCGCCTTTCTTTCATTGGGACACCGTACTGGATGGCTCCAGAGGTGGCAGCGGTGGC
  CCTGAAGGGTGGATACAATGAGCTGTGCGACATCTGGTCTCTTGGCATCACAGCCATCGAGCTGGCTGAG
  CTTCAGCCCCCTCTCTTTGATGTGCACCCTCTCAGGGTTCTCTTCCTCATGACCAAGAGCGGGTACCAGC
  CTCCTCGGCTGAAGGAAAAGAGCAGATGGTCATCTTCCTTCCACAACTTTGTCAAAGTCACCCTCACTAA
  GAACTCCAAGAAACGCCCCAGTGCCACCAAGATGCTCAGTCATCAGCTGGTGTCCCAGCCAGGGCTGAAC
  AGAGGCCTGATCTTGGATCTTCTTGACAAAATGAAGAATCCTGGGAAGGGGCTGCCTGTTGATATTGAAG
  ATGAGGAGCCTGAGCCACCCCCTGCCATCCCTCGGCGGATCAGATCTACCTACCGGGCCAGCTCACTAGG
  GATCCCAGATGCAGACTGCTGCCGGAGGCAAATGGAGTTCCAGAGGCCCAGATGCGTGGACTGCAGACCT
  CAGGCTGAAACTGTTCGCTTATATCCCCCTGCACACTTCGGGAGCACCAGTCCCAGGAGTCAGCTGTCAG
  ATTCTGATGACGACTATGATGATGTGGACATCCCTGCCCCTTCAGAGAACATACCTCCTCCACTGCCCCC
  CAAGCCCAAGTTTCGGTCTCCATCAGATGATGGTTCTGGAGGAATTAGGGACGATGGGCAATTGAGCCCA
  GGGGTTCTGGTTCGATGTGCAAGTGGGCCCCCTCCACGTACTCCCCGACCTGGACCTCCCCCAGCGACCT
  GCAGCCCTCACCTCACAGCTCGCTCAGATCCCTCATTGTGGAACCCAGCTGCTCCGGAGCCAGGCCAGCC
  CCCACTCGTACCCCCAAGGAAGGAAAAGATGAGGGGAAAGATGGAGAACGAGAAGCGCAGAGAGAAATAT
  CCCCTTCTTGTGAAATTATTCAATGGCTGTCCCCTTCAGATCCACAGCACAGCTGCCTGGACACATCCCT
  CTACAAAAGATCAGAACCTGCTCCTGGGGGCAGAGGAAGGCATTTTCATACTGAACCGGAATGATCAGGA
  GGCCACGCTGGAAATGATCTTCCCTGGCCGCACAACCTGGCTCTATTGCATCAACAACCTCCTCATGTCT
  CTCTCAGGGAAGACACCCTACCTGTATTCTCATAGCATCCTGGGCCTGCTTGAAAGGAAAGACGGCAGAA
  CGGGAAGCCCCATTGCTCACATTAGCCCTCACCGGTTACTGGCAAGAAAGAACATGGTCTCCAGTAAGAT
  CCAGGACACCAAAGGCTGCCGGGCTTGCTGTGTGGCTGAGAGTGCTAGCTCTGGGGGCCCATTCTTATGT
  GGGGCATTGGAGACGTCTGTGGTTTTGCTCCAGTGGTACCAACCAATGAACAAATTCTTGCTTGTCCGGC
  AGGTGCTGTTCCCGCTGCCCACGCCGCTGCCGGTGTTCACGCTGCTGACAACGCCGGGCTCCGAGCTGCC
  AGCTGTGTGCATCGGCGTGAGCCCGGGGCAGGCGGCAAAGTCGGTGCTCTTCCACACCGTGCGCTTCGGT
  GCGCTGTCCTGCTGGCTGGACGACTCCAGCACGGAGCACAAGGGACCAGTGCAGGTGATCCAAGTAAAGG
  AAGACATGGTGATGGTGTTGATGGATGGCTCTCTAAAGCTAGTGACCCCAGAGGGGGCACCAGCTCCTGG
  GCTCCGCACCCCTGAGATCCCCATGACAGAAGCGGTCGAGGCTGTGGCTATGGTTGAAGATCGACTCGAG
  GCCTTCTGGAAGCATGGAGTTCAGGTGTGGGCTCCAGGCTTGAAACAGCCGCTGCAGGAGCTGAGAGACC
  CCACCCTCACCTTCCGTCTGCTCTGCTCCCCCAGGCCGGTAGTGGTGGAGACAAGGCCCACGGATGACCC
  TACGGCCCCCAGCAACCTCTACATCCAGGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008279
• Insert Size: 2484 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008279.2, NP_032305.2
• RefSeq Size: 2699 bp
• RefSeq ORF: 2484 bp
• Locus ID: 26411
• UniProt ID: P70218
• Cytogenetics: 7 B1
• Gene Summary: Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.[UniProtKB/Swiss-Prot Function]