Dact1 (NM_001190466) Mouse Untagged Clone

CAT#: MC221808

Dact1 (untagged) - Mouse dapper homolog 1, antagonist of beta-catenin (xenopus) (Dact1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dact1
• Synonyms: 4921528D17Rik; AI115603; DAPPER; DAPPER1; Frd1; FRODO; Frodo1; MDpr1; MTNG3; THYEX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221808 representing NM_001190466
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCGGACGCAGCGCGCGAGCCGGAGCCGCTGAGCCCCGGCCGGGGCGCGGAGGCCGAGGGGCGCT
  GGCGCGAGAGGGGCGAGGCGGACACGGAGCGGCAGCGTACCCGCGAGCGCCAGGAGGCCACGCTGGCGGG
  GCTGGCGGAGCTGGGGTACCTGCGGCAACGCCAAGAGCTGCTGGTGCGCGGTGCGCTGCGCTGCTCCGGG
  ACCGTGGGGACCGTCGCGCCGCGCTCCGGGGAGCTGCGGGGAGACGCGGCGCAGCGCAGCCGCCTGGAGG
  AGAAGTTCCTGGAAGAGAACATCTTGCTGCTGCGAAGGCAGTTGAATTGTTTGAGGAGAAGAGATGCCGG
  TTTGTTGAATCAGTTGCAAGAACTTGACAAGCAGATAAGTGACCTGAGACTGGATGTGGAGAAGACATCT
  GAAGAGCACCTGGAGACAGACAGCCGGCCTAGCTCAGGGTTTTATGAGCTGAGTGATGGAGCTTCGGGCT
  CCCTCTCTAACTCCTCCAACTCCGTGTTCAGTGAGTGTTTGTCCAGTTGCCATTCCAGCACCTGCTTCTG
  CAGCCCCTTGGAGGCGGCCTTGACCATCTCAGACGGTTGCCCCAAATCTGCAGATCTCATAGGATGGTTG
  GAATGTAAAGGCGGCCCCTGTGAAGACCAGGCCTCAGGGACAGTGTGCAGTTCCCCCTCCACACCACAAT
  TTAATTCCCTTGATGTCATTGCAGATGTGAATCCTAAATACCAGTGTGATCTTGTGTCTAAAAACGGGAA
  TGACGTATATCGCTACCCCAGTCCACTTCATGCTGTGGCTGTGCAGAGCCCAATGTTTCTCCTTTGTCTG
  ACGGGCAACACTCTGAGGGAAGAGGAGGGGCTTGGGAGCCATGCCAGCGACATCTGCATTGGATCTGAAC
  TGAACGCCACCAAAACAGACAATTCCCTGCCATCTCCAAGCAGTTTGTGGTCCGCTTCCCATCCTGCATC
  CAGTAAGAAAATGGATGGGTATATTTTGAGCCTCGTGCAGAAGAAAACACACCCTGTAAGGACCAATAAA
  CCTAGAACCAGTGTGAACGCTGACCCTACCAAGGGCCTTCTGAGGAATGGAAGTGTGTGTGTCAGGGCCC
  CTAGTGGCGTCCCACCGGGCAGTAGTGTGAACTTTAAGAATACAAAACAGATGTGTTTGCCCGCTGGGGG
  AATAACCTCTTTGGAAAACGGGCCATTCTCCCCTCCTAAGCAGAGGTCCAAAGACTCAAAGACAGACCAG
  TTAGAAAGCAAGAGGTTGGCTCTGCCGGAGAGCTGCTCGGCAGGCGCCGCCATGGAACCCCAAAGCAAGC
  ATGTGCCCAAAGCCGCCAAGGCAGCCTCTCAAGAGCTCACAAGGTGTCAAGCCGGGCTGGGGGAATCCAT
  GAAGGAAAGCAATCAGGCCTCCGCTGTTTCTCCTAAAACAAGTCCTGGCAGAGGCCCTGTCGCCCCCGCA
  GAGAGCAAAGCCCTGCAGCTCCCGAAAAAGATGTCGCAGAAGAACAGCCTCCAGGCTGTGCCCGCGCTGG
  ACAGGCCGGCCTTGGACTTCAAAAGCGAGGGCTCATCTCAAAGCCTCGAGGAAGGGCATCTGGTGAAAGC
  TCAGTTCATTCCGGGGCAGCAGGCGGCCGCCAGGCCTCACCGTGCACACAGGAACCCGGGTGTCGCAAGG
  AGCGCCACCTTGAAGGCCCGCGGCCAGGCAGCCATGGAACACGGCCTGCCCACCGTCAGGGAGAAACCGC
  GGGCAGCAGGCAAGAAGTGCCGTTTCCCAGACGACTCGGATACAAATAAGAAATTCAGGAAGACCTCCGC
  CAAGGGCCGGCGCAGTGGCGGGCTGCAGGACGCTGGCCTTCCCGGTAGGGCCCTGGGCACCGGCGGCCAT
  CGGGCGGGTAGCAGGGCGCACGCGCATGGCCGGGAGCCCGTGGTGGCCAAACCGAAGCACAAGCGAACCG
  ACTACCGGCGGTGGAAATCGTCAGCCGAGGTCTCCTACGAAGAAGCCCTGCGGAGGGCCCGGAGGGCTCG
  CAGGGAGCACGGGGCTGCCTACCGGGTGGCTGTCGCCCTGCCTTACGCCAGCCCCTATGCCTACGTGCCC
  AGCGACTCCGAGTACTCGGCGGAGTGCGAGTCGCTCTTCCACTCCACGGTGGTGGACACCAGCGAGGACG
  AGCAGAGCAACTACACCACCAACTGCTTCGGCGACAGCGAGTCCAGCGTGAGCGAAGGCGACTTCGTGGG
  CGAGAGCACCACCACCAGCGACTCAGAGGAGAGCGGGGGTTTAATCTGGTCCCAGTTTGTCCAGACTCTC
  CCGATTCAAACGGTCACGGCCCCAGACCTCCACACCCGTCCCACAAAAACCTTTGTCAAAATCAAGGCTT
  CGCACAACCTCAAGAAGAAGATCCTCCGTTTCCGCTCTGGCTCTTTGAAACTGATGACTACCGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001190466
• Insert Size: 2448 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190466.1, NP_001177395.1
• RefSeq Size: 3761 bp
• RefSeq ORF: 2448 bp
• Locus ID: 59036
• Cytogenetics: 12 C3
• Gene Summary: Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB (By similarity). Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway. The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling (By similarity). May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.