Tpcn1 (NM_145853) Mouse Untagged Clone

CAT#: MC221794

Tpcn1 (untagged) - Mouse two pore channel 1 (Tpcn1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tpcn1
• Synonyms: 5730403B01Rik; mKIAA1169; Tpc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221794 representing NM_145853
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTAAGTTTAGATGACGATGTGCCGCTCATCCTGACCTTGGACGAGGCTGAGAGTGCTCCGCTGC
  CTCCTTCGAACAGCCTGGGCCAAGAGCAGCTGCCCAGCAAAAATGGGGGCAGCCACAGCATCCACAACTC
  CCAGGTCCCCAGTCTGGTCTCCGGAGCGGACAGCCCCCCCTCCAGTCCCACCGGACACAACTGGGAGATG
  AATTATCAAGAGGCGGCAATCTACCTCCAGGAAGGTCAGAACAACGACAAGTTCTTCACCCACCCCAAGG
  ATGCCAGAGCGCTGGCGGCCTACCTCTTCGTCCACAACCACTTCTTCTACATGATGGAGCTGCTCACGGC
  CCTGCTCCTGCTGCTGCTGTCGCTGTGCGAGTCCCCCGCTGTCCCCGTGCTCAAGCTGCACACTTACGTC
  CACGCCACGCTGGAACTCTTTGCCCTCATGGTGGTGGTATTTGAACTCTGCATGAAATTGCGGTGGCTGG
  GCTTCCACACGTTCGTCCGGCACAAACGTACCATGGTCAAGACGTCCGTCCTCGTGGTGCAGTTCATCGA
  GGCCATTGTGGTGCTGGTTCGGCAGACGTCCCACGTGCGGGTGACCCGGGCACTACGCTGCATTTTCCTG
  GTGGACTGTCGCTACTGTGGCGGTGTACGGCGCAACCTGCGGCAGATCTTCCAGTCTCTCCCACCCTTCA
  TGGACATCCTCCTGTTGCTGCTCTTCTTTATGATCATCTTTGCCATCCTCGGTTTCTACTTATTCTCCAC
  AAATCCTTCCGACCCCTACTTCAGCACCCTGGAGAACAGCATCGTCAACCTGTTCGTTCTCCTGACCACA
  GCCAACTTTCCAGATGTCATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGCGTCTTCTTCATTGTAT
  ACCTCTCCATTGAGCTGTACTTCATCATGAACCTGCTCCTGGCCGTGGTGTTCGACACCTTCAACGACAT
  TGAAAAGCACAAGTTCAAGTCTTTGCTGCTGCACAAACGGACCGCCATCCAGCATGCCTACGGCCTGCTT
  GCCAGCCAACGGAGGCCGGCTGGCATCTCCTACAGGCAGTTCGAAGGCTTAATGCGCTTCTACAAGCCCC
  GGATGAGTGCAAGGGAACGCTTCCTGACTTTCAAGGCCTTGAACCAGAGCAACACGCCTCTGCTCAGCCT
  GAAGGACTTCTATGATATTTACGAAGTCGCTGCTCTGCAGTGGAAGGCAAAGAGAAACAGACAGCATTGG
  TTTGATGAGCTCCCCCGGACAGCCTTCCTCATCTTCAAAGGGATTAACATCCTTGTGAATTCCAAGGCCT
  TCCAGTATTTCATGTACTTGGTGGTGGCTGTCAACGGTGTCTGGATCCTGGTGGAGACATTCATGTTGAA
  AGGTGGGAATTTCACCTCAAAGCATGTGCCCTGGAGTTACCTCGTGTTTCTTACCATCTATGGAGTTGAA
  CTGTTCATGAAGGTGGCTGGCCTGGGCCCTGTGGAGTACCTGTCCTCTGGATGGAACCTGTTCGATTTCT
  CGGTCACGGCATTCGCCTTCCTGGGACTGCTCGCACTGACGCTCAACATGGAACCCTTCTATTTCATTGT
  GGTCCTGCGTCCCCTTCAGCTGCTGAGGTTATTTAAACTGAAGAAACGCTACCGCAACGTGTTGGACACC
  ATGTTTGAGCTGCTGCCGCGGATGGCCAGCCTTGGCCTCACGCTGCTCACCTTCTACTATTCCTTCGCCA
  TCGTGGGCATGGAGTTCTTCAACGGGCGGCTGACCCCCAACTGCTGCAACACCAGCACAGTGGCCGACGC
  CTACCGGTTCATCAACCACACTGTGGGCAATAAGACCAAGGTAGAGGAAGGCTACTACTATCTCAACAAC
  TTTGACAACATCCTCAACAGCTTCGTGACCTTGTTTGAGCTCACCGTTGTCAACAATTGGTACATCATCA
  TGGAAGGCGTCACCTCGCAGACGTCCCACTGGAGCCGCCTGTACTTCATGACCTTTTACATAGTGACCAT
  GGTGGTGATGACCATTATCGTGGCCTTCATCCTGGAGGCCTTCGTCTTCCGCATGAACTACAGCCGCAAG
  AGCCAGGACTCGGAAGTGGACAGTGGCATCGTCATCGAGAAGGAAATGTCCAAGGAGGAGCTTATGGCCG
  TCCTGGAGCTTTATCGTGAGGAGCGAGGCACCTCCTCTGACGTGACCCGGCTGCTGGACACCCTCTCTCA
  GATGGAGAAATACCAGCAAAATTCCATGGTGTTTCTGGGACGGCGATCGAGAACCAAAAGTGACCTGAGT
  CTGAAGATGTACCAGGAGGAGATCCAGGAGTGGTACGAGGAGCATGCCCGGGAACAGGAGCAGCAGAAGC
  TCAGGGGCAGCGTGCCCGGCCCTGCAGCCCAGCAGCCCCCTGGCAGTCGCCAGCGCTCCCAGACTGTCAC
  CTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145853
• Insert Size: 2454 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145853.2, NP_665852.1
• RefSeq Size: 4666 bp
• RefSeq ORF: 2454 bp
• Locus ID: 252972
• UniProt ID: Q9EQJ0
• Cytogenetics: 5 F
• Gene Summary: Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal and endosomal membrane.[UniProtKB/Swiss-Prot Function]