Mark1 (NM_145515) Mouse Untagged Clone

CAT#: MC221734

Mark1 (untagged) - Mouse MAP/microtubule affinity-regulating kinase 1 (Mark1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mark1
• Synonyms: AW491150; B930025N23Rik; Emk3; mKIAA1477; mPar-1c; Par-1c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221734 representing NM_145515
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCGCGGACGCCATTGCCCACGGTGAACGAGCGGGACACGGAGAACCATACGTCTGTGGATGGCT
  ACACTGAAACACATATCCCGCCTGCCAAGTCAAGTAGCAGACAGAACCTTCCCCGGTGTAGAAACTCCAT
  TACGTCAGCGACAGATGAACAGCCTCACATCGGAAATTACCGCTTACAGAAAACAATAGGGAAAGGAAAT
  TTTGCCAAAGTGAAATTGGCAAGACATGTTCTAACAGGTAGAGAGGTCGCTGTGAAAATAATAGACAAAA
  CTCAGCTAAACCCTACCAGTCTACAGAAGTTGTTCCGAGAAGTACGAATAATGAAGATACTGAACCATCC
  TAACATAGTGAAACTGTTTGAAGTTATTGAAACAGAGAAGACACTCTATTTGGTCATGGAATACGCAAGT
  GGGGGTGAAGTCTTTGATTACTTAGTTGCCCACGGAAGAATGAAAGAGAAAGAGGCCCGTGCCAAATTCA
  GGCAGATTGTATCTGCTGTACAGTATTGCCATCAAAAATGCATTGTTCACCGTGATCTTAAGGCTGAAAA
  CCTTCTCCTTGATGCTGATATGAATATTAAAATTGCTGACTTTGGTTTTAGCAATGAATTCACAGTGGGG
  AACAAATTGGACACATTCTGTGGAAGCCCGCCTTATGCTGCCCCCGAGCTTTTCCAAGGCAAGAAGTACG
  ATGGGCCAGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTACACGCTGGTCAGTGGCTCGCTGCCGTT
  TGATGGCCAGAATCTAAAGGAACTTCGGGAGCGAGTCCTTCGAGGGAAGTACCGCATCCCCTTCTACATG
  TCCACAGACTGTGAGAATCTCCTGAAGAAACTGTTAGTGCTGAATCCCATAAAGCGGGGCAGCCTGGAGC
  AAATAATGAAAGACCGATGGATGAATGTTGGTCATGAGGAAGAAGAGCTAAAGCCGTACTCTGAGCCTGA
  GCTTGATCTCAGCGATGCAAAAAGAATAGACATTATGGTCACCATGGGTTTTGCACGAGATGAAATCAAT
  GATGCCTTAGTAAGTCAGAAATATGATGAAGTTATGGCTACTTACATCCTTCTAGGTAGAAAACCACCCG
  AGTTCGAAGGTGGTGAGTCACTGTCCAGTGGGAGCTTGTGCCAGCGGTCGCGGCCGAGCAGCGACCTGAA
  CAACAGCACCCTTCAGTCGCCTGCTCACCTGAAGGTCCAGCGGAGCATCTCGGCCAACCAGAAGCAGAGG
  CGCTTCAGCGACCACGCCGGCCCCTCCATCCCCCCTGCCGTCTCATACACCAAGAGGCCTCAGGCCAACA
  GCGTGGAGAGTGAACAGAAGGAGGAGTGGGGCAAAGACACAGCCCGCAGACTTGGCAGCACCACAGTGGG
  ATCAAAGAGCGAAGTCACAGCCAGCCCCTTGGTGGGGCCAGACAGGAAAAAGTCCACAGCGAGCCCAAGT
  AACAACGTATATTCTGGCGGTAGCATGGCGAGGAGGAATACGTATGTGTGTGAGAGGAGCACAGACCGGT
  ACGCAGCCTTGCAGAATGGAAGAGACAGCAGCCTTACAGAGATGTCTGCCAGCAGCATGTCCTCGGCGGG
  CTCCACCGTGGCCTCTGCTGGCCCCTCAGCACGTCCCCGCCACCAGAAATCTATGTCAACCTCTGGCCAC
  CCTATAAAAGTTACACTGCCAACCATTAAAGATGGCTCAGAAGCGTATCGGCCTGGTGCAGCTCAGAGAG
  TGCCTGCTGCTTCTCCGTCTGCTCACAGCATCAGTGCCAGCACTCCAGACAGGACCCGCTTCCCCCGAGG
  GAGCTCCAGCCGAAGCACTTTCCATGGGGAACAGCTCCGAGAGCGGCGCAGCGCGGCTTACAACGGACCA
  CCGGCCTCCCCATCCCACGAAACGGGTGCGTTTGCACATGCCAGAAGGGGCACGTCAACCGGCATCATCA
  GCAAAATCACCTCCAAGTTCGTCCGCAGGGATCCAAGTGAAGGCGAAGCCAGCGGCAGAGCCGACACAGC
  CAGAGGTTCATCGGGAGATCCCAAAGAGAGAGACAAGGACGAGGGCAAAGAGGCCAAGCCCCGCTCCCTG
  CGCTTCACCTGGAGCATGAAGACCACGAGCTCCATGGACCCCAACGACATGCTGAGGGAGATCCGCAAGG
  TGCTGGATGCCAACACCTGTGACTATGAGCAGAAAGAGAGGTTCCTGCTCTTCTGCGTTCACGGAGACGC
  TCGGCAGGACAGCCTCGTACAGTGGGAGATGGAGGTCTGCAAGTTGCCTCGGCTCTCACTCAACGGGGTC
  CGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTAAGAACATTGCATCCAAAATAGCAAATGAGCTTA
  AGCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145515
• Insert Size: 2388 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145515.2, NP_663490.2
• RefSeq Size: 4565 bp
• RefSeq ORF: 2388 bp
• Locus ID: 226778
• UniProt ID: Q8VHJ5
• Cytogenetics: 1 H5
• Gene Summary: Serine/threonine-protein kinase (By similarity). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (By similarity). Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).[UniProtKB/Swiss-Prot Function]