Trpc4ap (NM_001163452) Mouse Untagged Clone

CAT#: MC221708

Trpc4ap (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 associated protein (Trpc4ap), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpc4ap
• Synonyms: 4833429F06Rik; D2Ertd113e; mFLJ00177; Trrp4ap; truss
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221708 representing NM_001163452
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCGCCCGCAGCGGCTGGAGCTGGGGCCAGCCGGGGGAGGCGGTTGGCAGCTACCGCGGCGG
  CGTGGGGCGGCTGGGGAGGTCGACCGCGGCCGGGTAACATCCTGCTGCAGCTGCGGCAGGGCCAGCTCAC
  CGGCCGAGGCCTGGTCCGGGCGGTGCAGTTCACTGAGACCTTTTTGACCGAGAGGGACAAATTGTCCAAA
  TGGAGTGGAATTCCTCAGTTGCTCCTTAAGCTGTATGCCACCAGCCATCTGCACAGTGACTTCGTTGAGT
  GCCAAAGCATTCTCAAGGAGATTTCTCCTCTTCTCTCTATGGAGGCTATGGCGTTTGTAACTGAAGACAG
  GAAATTTACTCAAGAAGCTACATACCCAAATACATATATTTTTGACTTATTTGGAGGTGTTGATCTTCTT
  GTAGAAATTCTTATGAGGCCTACCATCTCTATCCGGGGACAGAAACTAAAAATAAGTGACGAGATGTCGA
  AGGACTGCTTGAGCATCCTGTACAATACCTGTGTCTGCACGGAAGGAGTAACGAAGCGTTTGGCAGAAAA
  GAATGATTTTGTGATTTTTCTGTTCACACTGATGACAAGTAAGAAGACATTCTTACAAACAGCGACTCTC
  ATTGAAGACATTTTGGGTGTTAAGAAGGAAATGATCCGGCTAGATGAAGTTCCCAATCTGAGTTCCTTAG
  TATCCAATTTCGATCAGCAGCAGCTTGCTAACTTCTGCAGGATCCTGGCTGTCACCATTTCAGAGATGGA
  CACGGGGAATGACGACAAACACACGCTTCTTGCCAAAAATGCTCAGCAGAAGAAGAGTTTGAGTTTGGGG
  CCTTCTGCAGCTGAAATCAACCAAGCTGCTCTTCTCAGCATCCCTGGCTTTGTGGAACGGCTTTGCAAAC
  TGGCAACTAGGAAAGTGTCAGAGTCAACGGGCACAGCCAGCTTCCTTCAAGAACTGGAGGAGTGGTACAC
  ATGGCTCGACAATGCGTTGGTGCTAGATGCCCTGATGCGAGTGGCCAATGAGGAGTCTGAACACAATCAA
  GCCCCCACTGTGTTCCCTTCTCTAGGCACTTCGGAGGAGGGTGGGCTGCCACACACCTCGGCCAGGGCGC
  AGCTGCCCCAGTCCATGAAGATTATGCATGAGATTATGTACAAACTGGAAGTGCTTTATGTCCTCTGCGT
  GCTGCTGATGGGGCGTCAGCGAAACCAGGTCCACAGGATGATTGCTGAGTTCAAGTTGATCCCAGGACTC
  AATAATTTGTTCGACAAACTGATTTGGAGGAAGCATTCAGCATCTGCCCTTGTCCTCCATGGCCACAACC
  AGAACTGTGACTGTAGCCCGGACATCACCTTGAAGATCCAGTTTTTGAGACTTCTCCAGAGTTTCAGTGA
  CCACCATGAGAACAAGTACTTGCTGCTCAACAACCAGGAGCTAAATGAGCTCAGTGCCATCTCGCTGAAA
  GCCAACATCCCTGAGGTGGAGGCTGTCCTCAACACTGACAGGAGTCTGGTGTGTGACGGCAAGAGGGGCC
  TACTAACTCGGCTGCTGCAGGTCATGAAGAAGGAGCCAGCGGAGTCTTCATTCAGGTTTTGGCAAGCCCG
  GGCTGTGGAAAGTTTCCTCCGAGGGACCACCTCCTATGCAGACCAGATGTTCCTGCTGAAGCGAGGCCTT
  CTGGAGCACATCTTATACTGCATTGTGGACAGTGAGTGCAAGTCTAGGGATGTACTCCAGAGCTACTTTG
  ATCTCCTGGGAGAGCTGATGAAGTTCAACGTCGATGCATTCAAGAGATTCAATAAATATATCAACACTGA
  TGCAAAGTTCCAGGTGTTCCTGAAACAGATCAACAGCTCGCTAGTGGACTCCAACATGCTGGTGCGCTGT
  GTCACTCTGTCACTGGACCGATTTGAAAACCAGGTGGACATGAAAGTGGCCGAGGTCCTGTCCGAGTGCC
  GCCTGCTTGCCTACATATCCCAGGTGCCCACGCAAATGTCCTTCCTCTTCCGCCTCATCAATATCATCCA
  TGTGCAGACGCTAACCCAGGAGAACGTCAGCTGCCTCAACACCAGCCTGGTAATCCTGATGCTGGCCCGA
  CGGAAAGAGCGGCTGCCCCTGTACCTGCGACTGCTACAGCGAATGGAGCACAGCAAGAAATACCCCGGCT
  TCCTGCTCAACAACTTCCACAACCTGCTGCGCTTTTGGCAGCAGCACTACCTGCACAAGGATAAGGACAG
  CACCTGCCTGGAGAATAGCTCCTGTATCAGCTTCTCGTACTGGAAGGAGACAGTGTCCATCTTGTTGAAC
  CCAGACCGCCAGTCGCCCTCTGCCCTCGTCAGCTACATTGAGGAGCCCTACATGGACATAGACAGAGACT
  TCACTGAGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163452
• Insert Size: 2394 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163452.1, NP_001156924.1
• RefSeq Size: 3219 bp
• RefSeq ORF: 2394 bp
• Locus ID: 56407
• UniProt ID: Q9JLV2
• Cytogenetics: 2 77.26 cM
• Gene Summary: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC (By similarity). Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).