Cdh5 (NM_009868) Mouse Untagged Clone

CAT#: MC221547

Cdh5 (untagged) - Mouse cadherin 5 (Cdh5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdh5
• Synonyms: 7B4; AA408225; Cd144; VE; VE-C; VE-Cad; Vec; VEcad; VECD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221547 representing NM_009868
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGGCTCACAGAGCTGGCCACTGCTTTGGGAGCCTTCCTGGGCCTCCTGGCAGTGGCAGCAATGG
  CAGGCCCTAACTTTCCCCAGATAGACACCCCCAACATGCTACCTGCCCACCATCGCCAAAAGAGAGACTG
  GATTTGGAATCAAATGCACATCGATGAAGAGAAAAATGAATCGCTGCCCCACTATGTGGGAAAGATCAAG
  TCCAACGTGAACCGCCAGAATGCTAAGTATGTGCTCCAAGGAGAGTTTGCTGGCAAGATCTTCGGGGTCG
  ATGCTAACACAGGGAATGTGCTTGCCTATGAGAGGCTAGACCGGGAGAAAGTCTCTGAGTACTTCCTTAC
  TGCCCTCATTGTGGACAAGAACACCAACAAAAACCTGGAACAACCTTCCAGCTTCACTGTCAAGGTGCAT
  GATATAAATGACAATTGGCCTGTGTTTTCGCACCAGGTATTCAACGCATCTGTGCCAGAGATGTCAGCTA
  TAGGGACCTCTGTCATCCGTGTGACAGCAGTGGATGCAGATGACCCCACTGTGGCCGGCCACGCCACTGT
  CTTGTACCAAATCGTGAAAGGAAATGAGTATTTCAGCATTGATAATTCTGGACTCATTTTCACAAAGATC
  AAAAACTTGGACCGAGAGAAACAGGCTGAATACAAGATCGTGGTGGAAACACAAGATGCCCTGGGTCTGC
  GGGGAGAGTCAGGCACAGCCACTGTGATGATCAGACTGGAGGACATCAATGACAACTTCCCCGTCTTTAC
  TCAATCCACATACACATTTTCGGTGCCTGAAGACATCCGAGTGGGCAAGCCCTTGGGCTTTCTGACTGTT
  GTGGACCCAGATGAGCCTCAGAACCGGATGACCAAGTACAGCATCATGCAGGGCGAGTACAGGGACACCT
  TCACCATTGAGACAGACCCCAAACGTAACGAGGGTATCATCAAACCCACGAAGTCCCTGGACTATGAAGT
  CATCCAGCAGTACACATTCTACATTGAGGCCACAGACCCCACTATCCGATACGAATACCTGAGCAGCACT
  TCAGGCAAAAACAAAGCCATGGTCACCATCAACGTCCTAGATGTGGATGAGCCCCCTGTCTTCCAGCGAC
  ACTTCTACCACTTCAAGCTGCCAGAAAACCAGAAGAAACCGCTGATCGGCACTGTGGTGGCCAAAGACCC
  TGACAAGGCTCAGCGCAGCATCGGGTACTCCATCCGCAAGACCAGTGACAGAGGCCAATTCTTCCGAATA
  ACCAAGCAGGGAAACATCTATAACGAGAAAGAACTGGACAGAGAAACCTACGCCTGGTATAACCTGACTG
  TGGAGGCCAACGAACTGGATTCTCGGGGTAACCCTGTAGGGAAAGAGTCCATTGTGCAGGTTTACATTGA
  AGTCTTGGATGAGAATGACAACCCTCCGGAATTTGCCCAGCCCTACGAACCTAAAGTGTGTGAGAATGCT
  GCCCAGGGCAAGCTGGTAGTACAGATCTCTGCAACAGACAAGGATGTGGTGCCAGTAAACCCAAAGTTCA
  AGTTTGCCCTGAAGAACGAGGACAGCAACTTCACCCTCATAAACAACCATGACAACACCGCCAACATCAC
  GGTCAAGTATGGGCAGTTTAATCGGGAGCATGCCAAGTTCCACTACCTGCCCGTGCTCATCTCAGACAAC
  GGGGTGCCCAGCCTGACTGGAACCAGCACGCTAACCGTGGGTGTGTGCAAGTGCAACGAGCAGGGCGAGT
  TCACCTTCTGTGAGGAGATGGCAGCCCAGGCGGGTGTCAGCATCCAGGCGCTGGTGGCCATCTTCCTCTG
  CATCCTCACCATCACAGTGATTACCTTGCTGATCATCCTGCGGAGGCGGATCCGGAAGCAGGCGCATGCT
  CATAGCAAGAGTGCGCTGGAGATTCACGAGCAGTTGGTCACTTACGATGAGGAGGGCGGTGGTGAGATGG
  ACACCACCAGCTATGATGTGTCAGTGCTTAATTCTGTGCGCGGTGGCTCCACTAAGCCCCTGCGCTCCAC
  GATGGACGCCCGGCCGGCAGTGTACACGCAGGTGCAGAAGCCGCCGCGCCTGGCGCCTGGACTGCACGGA
  GGGCCCAGGGAGATGGCCACCATGATCGACGTGAAGAAAGAAGAGGCAGACAATGACGGCGGTGGCCCCC
  CATACGACACACTGCACATCTACGGCTACGAGGGCGCAGAGTCCATCGCAGAGTCCCTCAGTTCCCTGAG
  CACCAATTCCTCCGACTCTGACATCGATTATGACTTCCTCAACGACTGGGGACCCAGGTTCAAGATGCTG
  GCTGAGCTGTACGGCTCAGATCCCCAGGAGGAACTCATCATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009868
• Insert Size: 2355 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009868.4, NP_033998.2
• RefSeq Size: 4004 bp
• RefSeq ORF: 2355 bp
• Locus ID: 12562
• UniProt ID: P55284
• Cytogenetics: 8 53.04 cM
• Gene Summary: This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Mice lacking the encoded protein die in utero due to vascular insufficiency, caused by increased endothelial apoptosis. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8. [provided by RefSeq, Oct 2015]