Qrich1 (NM_175143) Mouse Untagged Clone

CAT#: MC221500

Qrich1 (untagged) - Mouse glutamine-rich 1 (Qrich1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Qrich1
• Synonyms: 2610028H07Rik; b2b2404Clo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221500 representing NM_175143
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAATTCTCTAGAGAACACCATCTCATTTGAAGAGTACATCCGAGTAAAGGCGAGGTCTGTCCCGC
  AACACAGGATGAAGGAATTTCTGGACTCACTGGCTTCTAAGGGACCAGAAGCTCTTCAGGAGTTCCAGCA
  GACTGCCACCACCACCATGGTGTACCAGCAGGGTGGGAACTGCATTTATACAGACAGCACTGAAGTGGCT
  GGGTCTTTGCTTGAACTTGCTTGTCCAGTCACAACCAGTGTTCAACCACAAACTCAGCAAGAGCAGCAGA
  TCCAGGTTCAGCAGCCACAGCAGGTTCAGGTACAGGTGCAGGTGCAGCAGTCTCCACAACAGGTCTCAGC
  TCAGCAACTTTCCCCACAGTTCACCGTTCACCAGCCTGCTGAGCAACCCATCCAGGTCCAGGTGCAGATC
  CAGGGCCAAGCACCACAGTCCGCAGCCCCCTCCATTCAGACTCCATCTCTGCAGAGCCCCAGCCCCTCAC
  AGCTGCAAGCAGCTCAGATCCAGGTGCAGCACGTCCAGGCGGCCCAACAGATCCAGGCTGCAGAAATCCC
  AGAGGAGCATATCCCACATCAACAAATCCAGGCTCAGTTGGTAGCTGGCCAATCTCTTGCTGGGGGCCAG
  CAAATTCAAATTCAGACTGTGGGTGCTCTTTCCCCACCACCATCCCAGCAGGGCTCACCCAGGGAAGGGG
  AACGGAGGGTTGGCACAGCCAGTGTCCTTCAGCCAGTGAAGAAACGCAAAGTGGACATGCCTATCACCGT
  GTCCTACGCCATCTCAGGGCAGCCAGTGGCCACTGTGCTGGCCATTCCACAAGGCCAGCAACAGAGTTAT
  GTGTCTTTGAGGCCAGACTTACTGACAGTAGACAGTGCCCACCTGTACAGTGCCACTGGGACCATAACTA
  GCCCTACAGGAGAAACTTGGACCATCCCTGTTTATTCTGCCCAGCCCCGCGGGGATCCTCAGCAGCAGAG
  CATTACGCACATTGCCATTCCCCAGGAAGCCTACAATGCGGTTCATGTCAGTGGCTCACCTACGGCCCTG
  GCAGCTGTAAAGCTGGAGGATGACAAGGAGAAGATGGTGGGCACCACATCTGTAGTGAAAAACTCCCATG
  AAGAGGTAGTGCAGACCCTTGCAAACTCTCTCTTTCCAGCACAGTTCATGAATGGCAACATCCACATTCC
  AGTGGCTGTGCAGGCTGTAGCAGGCACATACCAGAATACGGCTCAGACTGTACATATATGGGACCCACAG
  CAGCAGCCACAACAGCAAACTGCACAAGAGCAGACACCACCACCACAACAGCAGCAGCAGCAGCTGCAGG
  TCACTTGTTCAGCACAGACTGTTCAGGTTGCTGAAGTTGAGCCACAGTCACAACCACAGCCTTCACCAGA
  GCTTTTGCTTCCAAATTCTTTGAAGCCAGAAGAAGGGCTTGAAGTATGGAAAAACTGGGCTCAGACCAAG
  AATGCTGAACTAGAAAAGGATGCTCAAAACAGATTGGCCCCCATTGGGAGGCGACAGTTGCTACGATTTC
  AGGAAGATCTGATCTCCTCTGCTGTGGCTGAATTGAATTATGGCCTTTGTTTAATGACACGAGAAGCTCG
  GAATGGAGAAGGTGAACCCTATGACCCAGATGTACTCTACTACATTTTCCTGTGTATACAGAAGTATCTT
  TTTGAAAATGGAAGAGTAGATGACATATTTTCTGATCTTTACTATGTTCGTTTCACCGAATGGCTACATG
  AAGTTCTGAAGGATGTACAGCCTCGGGTCACTCCACTTGGCTATGTGTTGCCCAGTCACGTGACTGAAGA
  GATGCTGTGGGAATGTAAGCAGCTTGGGGCACATTCCCCTTCCACACTGCTGACCACCCTCATGTTCTTT
  AACACCAAGTACTTCCTGTTGAAGACTGTAGATCAGCACATGAAGCTGGCTTTCTCTAAGGTCCTCCGAC
  AGACAAAGAAGAGCCCCTCGAATCCTAAGGATAAAAGCACGAGCATCCGCTACCTGAAGGCCCTCGGAAT
  ACACCAGACTGGCCAGAAAGTTACAGATGACATGTATGCAGAGCAAACAGAGAATCCAGAGAATCCACTA
  AGGTGTCCCATCAAGCTCTACGATTTCTACCTCTTTAAATGCCCTCAGAGTGTGAAAGGCCGAAATGACA
  CCTTTTACCTGACACCTGAGCCAGTTGTAGCTCCCAACAGCCCAATCTGGTACTCAGTCCAGCCTATCAG
  CAGAGAACAGATGGGACAGATGCTGACCCGGATCCTGGTTATACGAGAAATCCAAGAAGCCATTGCCGTG
  GCCAATGCAACCACCATGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175143
• Insert Size: 2334 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175143.5, NP_780352.2
• RefSeq Size: 3238 bp
• RefSeq ORF: 2334 bp
• Locus ID: 69232
• UniProt ID: Q3UA37
• Cytogenetics: 9 F2
• Gene Summary: Transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PubMed:33384352). Controls the outcome of the unfolded protein response (UPR), an ER-stress response pathway that either promotes recovery of ER homeostasis and cell survival, or triggers the terminal UPR which elicits programmed cell death when ER stress is prolonged and unresolved (PubMed:33384352). ER stress induces QRICH1 translation by a ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced QRICH1 regulates a transcriptional program associated with protein translation, protein secretion-mediated proteotoxicity and cell death during the terminal UPR (By similarity). May cooperate with ATF4 transcription factor signaling to regulate ER homeostasis which is critical for cell viability (By similarity). Upregulates CASP3/caspase-3 activity in epithelial cells under ER stress. Central regulator of proteotoxicity associated with ER stress-mediated inflammatory diseases in the intestines and liver (PubMed:33384352). Involved in chondrocyte hypertrophy, a process required for normal longitudinal bone growth (PubMed:30281152).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant lacks an exon in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.