Ift80 (NM_026641) Mouse Untagged Clone

CAT#: MC221499

Ift80 (untagged) - Mouse intraflagellar transport 80 homolog (Chlamydomonas) (Ift80), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ift80
• Synonyms: 4921524P20Rik; mKIAA1374; Wdr56
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221499 representing NM_026641
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGAAGATATCTCTCTCAAAGGAACCAAAGCATCAAGAATTAGTGAGCTGTGTGGGTTGGACTA
  CGGCGGAGGAGCTGTACTCATGCAGTGACGACCACCAGATCGTCAAGTGGAACCTGCTAACCAGTGAAAC
  CAGCCTAATAGTCAAGCTGCCTGATGACATCTACCCGATAGACCTTCACTGGTTTCCAAAGAGTTTAGGC
  ATAAAGAAACAGACGCAGGCAGAAAGCTTCGTCCTCACAAGCTCTGATGGTAAGTTTCATCTGATTTCCA
  AGTTAGGAAGAGTGGAAAAGAGTGTAGAAGCTCACTGTGGGGCTGTGCTCGCAGGACGATGGAATTATGA
  GGGGACTGCATTAGTGACAGTTGGAGAAGATGGACAAGTGAAAATCTGGTCAAAGACAGGGATGCTCAGA
  TCAACTTTAGCCCAGCAAGGAACACCAGTGTACTCAGTAGCCTGGGGCCCTGATTCAGAAAAGGTTCTTT
  ATACAGCAGGCAAACAGCTCATCATTAAACCTCTTCAACCAAATGCTAAAGTTTTACAGTGGAAAGCCCA
  TGATGGCATCATTTTAAAAGTCGACTGGAACTCAGTGAATGACCTTATTTTATCTGCGGGTGAAGACTGT
  AAGTATAAGGTTTGGGATAGTTATGGCCGCGTGCTGTACGGCTCCCAGCCTCATGAGCACCCCATCACCT
  CAGTGGCCTGGGCTCCAGATGGGGAGTTATTTGCCGTTGGATCGTTTCATACGTTACGCTTGTGTGATAA
  AACTGGGTGGTCATATGCTTTAGAGAAACCCAACACTGGCAGCATATTTAACATTGCGTGGTCTATTGAT
  GGCACTCAGATCGCGGGAGCCTGTGGGAACGGGCATGTTGTCTTTGCACATGTGGTGGAGCAGCGCTGGG
  AGTGGAAAAATTTTCAAGTAACGCTAACGAAGAGAAGGACCATGCAGGTTCGCAACGTTCTCAATGATGC
  CGTGGATCTCCTGGAGTTCCGGGACAGAGTCATTAAAGCATCCTTAAACCACGCACACTTGGTGGTCTCC
  ACGTCCCTGCAGTGCTACGTGTTCTCTACGAAGAACTGGAATACACCACTTATATTTGATCTCAAAGAAG
  GAACTGTTAGTTTAATTCTGCAGGCAGAAAGACATTTTCTTCTGGTGGATGGCGGGGGTATCTACCTGCA
  CTCTTATGAAGGGCGCTTCATTTCTTCTCCAAAGTTTCCTGGCATGAGAACAGACATCCTGAACGCTCAG
  ACTGTGTCTCTGAGCAATGACACGATAGCAATAAAGGACAAAGCTGACGAGAAGATAATCTTCCTCTTTG
  AGGCGTCAACTGGAAAACCCCTAGGGGATGGGAAACTTCTTTCTCATAAGAATGAAATCTCAGAAATTGC
  TCTGGATCAAAAAGGACTTACCAATGACAGAAAAATTGCTTTCATTGACAAGAACAGAGATCTCTACATC
  ACCTCTGTGAAACGATTTGGGAAAGAAGAACAGATTATTAAACTTGGAACCATGGTACATACTTTGGCAT
  GGTGTGATACATGCAATATCCTCTGTGGAATTCAAGATACTCGATTCACAGTATGGTATTACCCTAATAC
  CATTTATGTGGACAGAGACATTCTGCCTAAAACATTGTACGAAAGAGATGCAAGTGAATATAGTAAAAAC
  CCGCATATAGTGAGTTTTGTTGGAAATCAGGTAACTATAAGAAGAGCTGATGGCTCCCTGGTGCACATCA
  GCATATCACCGTACCCCGCCATCCTCCATGAGTATGTGAGCAGCTCCAAGTGGGAAGAGGCTGTAAGGCT
  TTGCCGCTTTGTTAAGGAGCAAAGCATGTGGGCTTGCCTAGCGGCTATGGCTGTTGCTAATCGAGATATG
  GTCACTGCAGAAATAGCCTATGCAGCAGTTGGGGAAATTGATAAAGTTCGGTACATCAATGCTATAAAAG
  ATCTTCCATCTAGAGAGTCAAAAATGGCCCACATACTCATGTTTAGTGGGAACATCCAGGAGGCTGAAAC
  AGTCCTGCTCCAGGCTGGCCTTGTTTATCAAGCCATACAGATCAACATTAATCTCTATAACTGGGAAAGG
  GCGCTGGAGTTGGCTGTAAAATACAAAACCCACGTTGATACAGTGCTCGCTTATCGTCAGAAGTTCTTGG
  ACACATTTGGTAAACAGGAAACTAATAAACGATATTTACAATATGCAGAGGGCCTCCAAATAGACTGGGA
  GAAAATCAAAGCCAAAATTGAGATGGAAATTACTAAAGAACGAGACCGATCATCCAGCGGCCAGTCCAGC
  AAGAGTGTGGGCTTAAAGCACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026641
• Insert Size: 2334 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026641.2, NP_080917.1
• RefSeq Size: 4090 bp
• RefSeq ORF: 2334 bp
• Locus ID: 68259
• UniProt ID: Q8K057
• Cytogenetics: 3 E1
• Gene Summary: Component of the intraflagellar transport (IFT) complex B, which is essential for the development and maintenance of motile and sensory cilia.[UniProtKB/Swiss-Prot Function]