Ece2 (NM_139293) Mouse Untagged Clone

CAT#: MC221455

Ece2 (untagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ece2
• Synonyms: 1810009K13Rik; 6330509A19Rik; 9630025D12Rik; BB127715; Ece-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221455 representing NM_139293
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGTCGCGCTGCACGAGTTGGGTGGCGGAGGCAGTATGGTGGAGTACAAACGTGCTAAGCTTCGGG
  ATGAAGAATCACCAGAGATTACAGTTGAAGGCAGGGCCACCCGGGACTCACTGGAGGTGGGATTCCAGAA
  GAGGACAAGACAACTGTTTGGTTCACACACACAGTTGGAGCTGGTCTTGGCAGGCCTCATTCTAGTGTTG
  GCTGCCCTTCTTTTGGGCTGCCTCGTGGCTCTGTGGGTCCACAGAGACCCAGCCCATAGCACCTGCGTCA
  CAGAAGCCTGCATTCGAGTAGCTGGAAAAATCCTGGAGTCTCTAGACCGTGGGGTGAGCCCCTGTCAGGA
  CTTTTACCAGTTCTCCTGTGGAGGCTGGATTCGAAGAAACCCTCTACCCAATGGACGTTCTCGCTGGAAC
  ACCTTCAACAGCCTCTGGGACCAGAACCAGGCCATACTGAAGCACCTACTTGAGAACACCACTTTCAATT
  CCAGCAGTGAAGCTGAGAGGAAGACTCGGAGTTTCTACCTGTCCTGCCTACAGTCGGAGCGCATTGAGAA
  GCTAGGAGCCAAGCCACTTAGAGACCTCATTGACAAGATCGGTGGTTGGAACATAACGGGGCCTTGGGAC
  GAGGACAGCTTCATGGATGTGCTCAAGGCAGTCGCAGGGACCTACAGAGCCACCCCCTTCTTCACCGTCT
  ACGTCAGTGCTGATTCTAAGAGTTCTAACAGCAATATCATCCAGGTGGACCAGTCTGGGCTTTTTCTGCC
  CTCTCGAGATTACTACCTAAATAGAACTGCCAATGAGAAAGTTCTCACTGCCTACCTGGACTACATGGTG
  GAGCTGGGAGTGCTGCTGGGTGGACAGCCGACCTCCACTCGGGAGCAGATGCAGCAGGTGCTGGAGCTGG
  AGATACAGCTGGCTAACATCACTGTGCCCCAGGACCAGCGGCGTGATGAGGAGAAGATCTATCACAAGAT
  GAGCATCTCAGAGCTGCAGGCTCTCGCGCCCGCCGTGGACTGGCTGGAGTTCCTTTCTTTCTTGTTATCG
  CCACTTGAGTTGGGTGATTCTGAGCCTGTGGTGGTGTATGGGACTGAGTATTTACAGCAGGTGTCGGAGC
  TCATCAACCGTACTGAACCAAGCATCCTGAACAATTACCTAATTTGGAACCTGGTACAGAAGACGACCTC
  AAGCCTTGACCAGCGCTTTGAGACTGCACAGGAGAAACTGCTGGAGACCCTCTACGGTACCAAGAAGTCC
  TGCACTCCGAGGTGGCAGACCTGCATCTCCAATACAGATGATGCCCTTGGCTTTGCTCTGGGTTCACTCT
  TTGTGAAAGCCACATTTGACCGACAAAGCAAGGAAATCGCCGAGGGGATGATCAATGAAATCCGCTCTGC
  TTTTGAGGAGACCCTGGGAGACTTGGTTTGGATGGATGAGAAGACCCGGCTGGCAGCCAAGGAGAAAGCA
  GATGCCATCTATGATATGATTGGTTTCCCTGATTTCATCCTGGAGCCCAAAGAGCTGGATGATGTTTATG
  ATGGGTATGAAGTCTCTGAAGATTCTTTTTTCCAAAACATGTTGAATCTGTACAACTTCTCAGCTAAGGT
  GATGGCTGACCAGCTCCGCAAACCTCCCAGCCGAGACCAGTGGAGCATGACACCTCAGACCGTGAACGCT
  TACTACCTTCCAACCAAGAATGAAATCGTCTTCCCTGCTGGCATCTTGCAGGCCCCCTTCTATGCTCACA
  ACCATCCAAAGGCCTTGAACTTTGGTGGCATCGGCGTAGTGATGGGCCATGAGTTGACACATGCCTTTGA
  TGACCAAGGGCGTGAGTATGACAAAGAAGGGAATCTGCGTCCTTGGTGGCAGAATGAGTCACTGACGGCT
  TTCCAGAACCATACAGCCTGCATGGAAGAACAGTACAGCCAGTACCAGGTCAATGGAGAGAGGCTCAATG
  GACTCCAGACCCTGGGGGAAAACATCGCCGATAATGGGGGCCTTAAGGCTGCTTACAATGCTTACAAAGC
  ATGGCTGAGAAAGCATGGGGAGGAGCAGCCGCTGCCTGCTGTGGGGCTCACCAATCACCAGCTTTTCTTC
  GTGGGATTTGCTCAGGTGTGGTGCTCGGTCCGCACACCAGAGAGCTCTCACGAGGGGCTGGTGACCGACC
  CCCACAGCCCTGCCCGTTTCCGAGTGCTGGGCACTCTCTCCAACTCCCGAGACTTCCTTCGGCACTTCGG
  CTGCCCTGTCGGCTCCCCCATGAACCCAGGGCAGCTATGTGAGGTGTGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_139293
• Insert Size: 2292 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139293.2, NP_647454.2
• RefSeq Size: 3129 bp
• RefSeq ORF: 2292 bp
• Locus ID: 107522
• UniProt ID: B2RQR8
• Cytogenetics: 16 B1
• Gene Summary: Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides (By similarity). May play a role in amyloid-beta processing (PubMed:12464614).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and CDS compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.