Mmel1 (NM_013783) Mouse Untagged Clone

CAT#: MC221441

Mmel1 (untagged) - Mouse membrane metallo-endopeptidase-like 1 (Mmel1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mmel1
• Synonyms: Mell1; NEP2; NEPII; Nl1; SEP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221441 representing NM_013783
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGAGAGCAGGCTGGTGTCGGAAGAAGTCCCCAGGCTTCGTGGAGTATGGGCTGATGGTGCTGC
  TGCTGCTGCTGTTGCTGGGAGCCATAGTGACTCTGGGTGTCTTCTACAGCATAGGGAAGCAGCTGCCCCT
  CTTAACTAGCCTGCTACACTTCTCCTGGGATGAGAGGACGGTTGTAAAACGAGCCCTCAGGGATTCATCA
  CTGAAAAGTGACATCTGCACCACCCCAAGCTGTGTGATAGCAGCTGCCAGAATCCTCGAAAACATGGACC
  AATCGAGGAACCCCTGTGAAAACTTCTACCAGTACGCTTGCGGAGGCTGGCTGAGGCACCACGTGATCCC
  AGAGACCAACTCCAGATACAGCGTCTTTGACATCCTTCGGGACGAGCTGGAGGTTATCCTCAAAGGGGTG
  CTGGAGGATTCCACTTCCCAGCATCGCCCGGCCGTGGAGAAGGCCAAGACACTATATCGCTCCTGCATGA
  ACCAAAGTGTGATCGAGAAGAGAGACTCTGAGCCCCTGCTGAGCGTCTTAAAAATGGTAGGAGGTTGGCC
  TGTGGCCATGGATAAGTGGAACGAGACCATGGGCCTCAAGTGGGAACTGGAGCGACAGTTGGCTGTGTTG
  AACTCGCAGTTCAACAGGCGGGTCCTCATCGACCTCTTCATCTGGAATGACGACCAGAACTCCAGCCGGC
  ATGTCATCTACATAGACCAGCCCACCTTGGGCATGCCATCCCGGGAGTACTATTTCCAGGAGGACAACAA
  CCACAAGGTACGGAAAGCCTACCTGGAGTTCATGACGTCAGTGGCCACTATGCTTAGGAAAGACCAGAAC
  CTGTCCAAGGAGAGCGCCATGGTGCGGGAGGAGATGGCGGAGGTGCTGGAACTGGAGACGCATCTGGCCA
  ACGCCACAGTCCCCCAGGAGAAAAGGCATGATGTCACTGCCCTGTACCACCGAATGGACCTGATGGAGCT
  ACAGGAAAGGTTTGGTCTGAAGGGGTTTAACTGGACTCTCTTCATACAAAACGTGTTGTCTTCTGTGGAA
  GTCGAGCTGTTCCCAGATGAGGAGGTGGTGGTCTACGGCATCCCCTACCTGGAGAATCTGGAGGATATCA
  TTGATAGCTACTCAGCACGGACCATGCAGAACTACCTGGTATGGCGCCTGGTGCTAGATCGAATTGGCAG
  CCTGAGCCAGAGATTCAAAGAGGCGCGTGTGGACTACCGCAAGGCGCTGTACGGCACGACCGTGGAGGAG
  GTACGCTGGCGAGAGTGTGTCAGCTATGTCAACAGTAACATGGAGAGCGCCGTGGGCTCCCTCTACATCA
  AGCGGGCCTTCTCCAAGGACAGCAAGAGCACGGTCAGAGAGCTGATTGAGAAGATAAGGTCCGTGTTTGT
  GGATAACCTGGATGAGCTGAACTGGATGGACGAGGAATCCAAGAAGAAGGCCCAGGAAAAGGCCATGAAT
  ATACGGGAACAGATTGGCTACCCTGACTACATTTTGGAAGATAACAATAAACACCTGGATGAGGAATACT
  CCAGTTTGACTTTCTATGAGGACCTGTATTTTGAGAACGGACTTCAGAACCTCAAGAACAATGCCCAGAG
  GAGCCTCAAGAAGCTTCGGGAAAAGGTGGACCAGAATCTCTGGATCATCGGGGCTGCAGTGGTCAATGCA
  TTCTACTCCCCAAACAGAAACCAGATCGTCTTTCCAGCAGGGATTCTCCAGCCGCCCTTCTTCAGCAAGG
  ACCAACCACAGTCCTTGAATTTTGGGGGCATCGGGATGGTGATTGGGCACGAGATCACACACGGCTTTGA
  TGATAATGGTCGTAACTTTGACAAGAACGGCAACATGCTGGACTGGTGGAGTAACTTCTCGGCCCGGCAC
  TTCCAACAGCAGTCGCAATGCATGATCTATCAGTACGGCAACTTCTCTTGGGAACTAGCAGACAACCAGA
  ATGTGAACGGATTCAGTACCCTCGGGGAGAACATTGCCGACAACGGAGGTGTGCGACAGGCATACAAGGC
  TTACCTACGGTGGCTGGCTGATGGCGGCAAAGATCAGCGACTGCCGGGACTGAACCTGACCTATGCCCAG
  CTTTTCTTCATCAACTATGCCCAGGTGTGGTGTGGGTCCTATAGGCCGGAGTTCGCCGTCCAGTCCATCA
  AGACGGACGTCCACAGTCCTCTTAAGTACAGGGTGCTGGGCTCACTACAGAACCTGCCAGGCTTCTCTGA
  GGCATTCCACTGCCCACGAGGCAGCCCCATGCACCCCATGAAGCGATGTCGCATCTGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013783
• Insert Size: 2301 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013783.2, NP_038811.2
• RefSeq Size: 2948 bp
• RefSeq ORF: 2301 bp
• Locus ID: 27390
• UniProt ID: Q9JLI3
• Cytogenetics: 4 E2
• Gene Summary: Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond.[UniProtKB/Swiss-Prot Function]