Jup (NM_010593) Mouse Untagged Clone

CAT#: MC221283

Jup (untagged) - Mouse junction plakoglobin (Jup), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Jup
• Synonyms: Ctnng; D930025P04Rik; PG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221283 representing NM_010593
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGATGAACCTTATTGAGCAGCCCATCAAGGTGACAGAGTGGCAACAGACATACACCTACGACT
  CGGGCATCCACTCCGGCGTCAATACCTGTGTGCCCTCTGTAAGCAGCAAGGGCATCATGGATGAGGATGA
  TGCCTGCGGCAGACAGTACACACTCAAGAAGACTACCACCTATACACAAGGGGTGCCACAGAACCAAGGT
  GACCTGGAATACCAGATGTCCACAACGGCCAGAGCCAAGCGGGTGCGGGAGGCCATGTGTCCAGGGGTCT
  CAGGCGAGGACAGTTCTCTACTGCTGGCCACCCAGGTGGAGGGGCAGACAACCAATCTGCAGCGCCTGGC
  CGAACCATCCCAGTTGCTCAAGTCGGCCATCGTCCATCTCATCAACTACCAGGATGATGCAGAGCTGGCC
  ACCCGGGCTCTGCCTGAGCTCACCAAGCTGCTCAACGATGAGGACCCGGTAGTGGTGACCAAGGCGGCTA
  TGATCGTGAACCAGCTGTCCAAGAAGGAGGCATCCCGCCGGGCTCTAATGGGCTCGCCCCAGCTGGTGGC
  GGCGGTGGTGCGGACTATGCAGAACACCAGTGACCTGGATACTGCCCGGTGTACCACCAGCATCCTGCAC
  AACCTCTCTCACCACCGAGAGGGCTTGCTCGCCATATTCAAGTCTGGAGGCATCCCTGCCCTAGTCCGAA
  TGCTCAGTTCCCCCGTAGAATCTGTCCTGTTCTACGCCATCACCACACTGCACAACCTGCTGCTCTATCA
  GGAGGGCGCCAAGATGGCGGTGCGTCTGGCAGACGGGCTGCAGAAGATGGTGCCCCTACTCAACAAGAAC
  AACCCTAAGTTCCTGGCCATCACTACTGACTGCCTGCAGCTCCTGGCCTATGGCAACCAGGAGAGCAAGC
  TGATCATCCTGGCCAATGGGGGACCCCAAGGGCTTGTGCAGATCATGAGGAACTACAGCTACGAGAAGCT
  TCTGTGGACCACCAGCCGTGTGCTCAAGGTGCTCTCCGTGTGTCCTAGCAACAAGCCCGCCATCGTGGAG
  GCTGGTGGGATGCAGGCTCTGGGCAAGCACTTGACAAGCAACAGTCCCCGCCTGGTGCAGAACTGCCTGT
  GGACTCTGCGCAATCTCTCGGATGTAGCCACCAAGCAGGAGGGCCTGGAGAGTGTGCTGAAGATCCTGGT
  CAACCAACTGAGTGTAGATGACGTCAACGTCCTCACCTGTGCCACGGGCACCCTCTCCAACCTGACCTGC
  AACAACAGCAAAAACAAGACGCTGGTGACACAGAACAGTGGCGTGGAGGCGCTCATCCACGCCATCCTGA
  GAGCCGGAGACAAAGATGACATCACAGAGCCCGCTGTCTGTGCCCTGCGCCACCTTACCAGCCGTCACCC
  CGAGGCTGAGATGGCCCAGAACTCTGTGCGCCTCAACTATGGAATCCCGGCCATTGTGAAACTGCTCAAC
  CAGCCAAACCAGTGGCCACTGGTCAAGGCAACTATTGGCCTGATCCGGAACCTGGCTCTGTGCCCAGCCA
  ACCATGCCCCTCTGCAGGAGGCCGCGGTCATTCCCCGCCTCGTCCAGCTGTTGGTCAAGGCCCACCAGGA
  CGCCCAGCGCCATGTGGCTGCAGGCACCCAGCAGCCCTACACGGATGGTGTGAGGATGGAGGAGATCGTG
  GAAGGCTGCACCGGAGCCCTGCACATCCTCGCCCGGGATCCCATGAACCGCATGGAGATCTTCCGGCTCA
  ACACCATTCCCCTGTTTGTCCAGCTCCTGTACTCCTCTGTGGAGAACATCCAGCGTGTGGCTGCGGGAGT
  GCTCTGCGAGCTGGCCCAGGACAAGGAGGCTGCCGACGCCATTGATGCGGAGGGCGCCTCTGCCCCTCTC
  ATGGAGCTACTCCATTCCCGCAATGAGGGCACCGCGACCTACGCTGCTGCTGTCCTGTTCCGCATCTCTG
  AGGACAAGAACCCAGATTACCGTAAGCGAGTGTCTGTGGAGCTCACCAATTCCCTCTTCAAGCATGACCC
  AGCTGCCTGGGAGGCTGCCCAGAGTATGATCCCGATCAACGAACCCTATGCAGACGACATGGATGCCACC
  TACCGCCCCATGTACTCCAGCGATGTGCCTCTGGACCCTCTGGACATGCACATGGACCTGGATGGAGACT
  ACCCCATGGACACCTACAGTGACGGCCTGAGGCCTCCCTACCCCACTGCAGACCACATGCTGGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010593
• Insert Size: 2238 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010593.2, NP_034723.1
• RefSeq Size: 5009 bp
• RefSeq ORF: 2238 bp
• Locus ID: 16480
• UniProt ID: Q02257
• Cytogenetics: 11 63.47 cM
• Gene Summary: Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.[UniProtKB/Swiss-Prot Function]