Ezh2 (NM_007971) Mouse Untagged Clone

CAT#: MC221274

Ezh2 (untagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ezh2
• Synonyms: Enx-1; Enx1h; KMT6; mKIAA4065
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221274 representing NM_007971
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAGACTGGGAAGAAATCTGAGAAGGGACCGGTTTGTTGGCGGAAGCGTGTAAAATCAGAGTACA
  TGAGACTGAGACAGCTCAAGAGGTTCAGAAGAGCTGATGAAGTAAAGACTATGTTTAGTTCCAATCGTCA
  GAAAATTTTGGAAAGAACTGAAACCTTAAACCAAGAGTGGAAGCAGCGGAGGATACAGCCTGTGCACATC
  ATGACTTCTGTGAGCTCATTGCGCGGGACTAGGGAGTGTTCAGTCACCAGTGACTTGGATTTTCCAGCAC
  AAGTCATCCCGTTAAAGACCCTGAATGCAGTCGCCTCGGTGCCTATAATGTACTCTTGGTCGCCCTTACA
  ACAGAATTTTATGGTGGAAGACGAAACTGTTTTACATAACATTCCTTATATGGGGGATGAAGTTCTGGAT
  CAGGATGGCACTTTCATTGAAGAACTAATAAAAAATTATGATGGAAAAGTGCATGGTGACAGAGAATGTG
  GATTTATAAATGATGAAATTTTTGTGGAGTTGGTAAATGCTCTTGGTCAATATAATGATGATGATGATGA
  CGATGATGGAGATGATCCAGATGAAAGAGAAGAAAAACAGAAAGATCTAGAGGATAATCGAGATGATAAA
  GAAACTTGCCCACCTCGGAAATTTCCTGCTGATAAAATATTTGAAGCCATTTCCTCAATGTTTCCAGATA
  AGGGCACCGCAGAAGAACTGAAAGAAAAATATAAAGAACTCACGGAGCAGCAGCTCCCAGGTGCTCTGCC
  TCCTGAATGTACTCCAAACATCGATGGACCAAATGCCAAATCTGTTCAGAGGGAGCAAAGCTTGCATTCA
  TTTCATACGCTCTTCTGTCGACGATGTTTTAAGTATGACTGCTTCCTACATCCCTTCCATGCAACACCCA
  ACACATATAAGAGGAAGAACACAGAAACAGCTTTGGACAACAAGCCTTGTGGACCACAGTGTTACCAGCA
  TCTGGAGGGAGCTAAGGAGTTTGCTGCTGCTCTTACTGCTGAGCGTATAAAGACACCACCTAAACGCCCA
  GGGGGCCGCAGAAGAGGAAGACTTCCGAATAACAGTAGCAGACCCAGCACCCCCACCATCAGTGTGCTGG
  AGTCAAAGGATACAGACAGTGACAGAGAAGCAGGGACTGAAACTGGGGGAGAGAACAATGATAAAGAAGA
  AGAAGAGAAAAAAGATGAGACGTCCAGCTCCTCTGAAGCAAATTCTCGGTGTCAAACACCAATAAAGATG
  AAGCCAAATATTGAACCTCCTGAGAATGTGGAGTGGAGTGGTGCTGAAGCCTCCATGTTTAGAGTCCTCA
  TTGGTACTTACTACGATAACTTTTGTGCCATTGCTAGGCTAATTGGGACCAAAACATGTAGACAGGTGTA
  TGAGTTTAGAGTCAAGGAGTCCAGTATCATAGCACCTGTTCCCACTGAGGATGTAGACACTCCTCCAAGA
  AAGAAGAAAAGGAAACATCGGTTGTGGGCTGCACACTGCAGAAAGATACAACTGAAAAAGGACGGCTCCT
  CTAACCATGTTTACAACTATCAACCCTGTGACCATCCACGGCAGCCTTGTGACAGTTCGTGCCCTTGTGT
  GATAGCACAAAATTTTTGTGAAAAGTTTTGTCAATGTAGTTCAGAGTGTCAAAACCGCTTTCCTGGATGT
  CGGTGCAAAGCACAATGCAACACCAAACAGTGTCCATGCTACCTGGCTGTCCGAGAGTGTGACCCTGACC
  TCTGTCTCACGTGTGGAGCTGCTGACCATTGGGACAGTAAAAATGTATCCTGTAAGAACTGTAGCATTCA
  GCGGGGCTCTAAAAAGCACTTACTGCTGGCACCGTCTGATGTGGCAGGCTGGGGCATCTTTATCAAAGAT
  CCTGTACAGAAAAATGAATTCATCTCAGAATACTGTGGGGAGATTATTTCTCAGGATGAAGCAGACAGAA
  GAGGAAAAGTGTATGACAAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGC
  AACCCGAAAGGGCAACAAAATTCGTTTTGCTAATCATTCAGTAAATCCAAACTGCTATGCAAAAGTTATG
  ATGGTTAATGGTGACCACAGGATAGGCATCTTTGCTAAGAGGGCTATCCAGACTGGTGAAGAGTTGTTTT
  TTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTGGGCATCGAACGAGAAATGGAAATCCCTTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007971
• Insert Size: 2241 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007971.2, NP_031997.2
• RefSeq Size: 2665 bp
• RefSeq ORF: 2241 bp
• Locus ID: 14056
• UniProt ID: Q61188
• Cytogenetics: 6 22.92 cM
• Gene Summary: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). This isoform is also known as ENX-1A.