Vps52 (NM_172620) Mouse Untagged Clone

CAT#: MC221127

Vps52 (untagged) - Mouse vacuolar protein sorting 52 (yeast) (Vps52), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps52
• Synonyms: ARE1; D130068D18; D430041K17Rik; SAC2; Sacm2l; tclw5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221127 representing NM_172620
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCGCAGCGACCATGGCGGCCGCTGCCCGGGAGCTGGTGTTGCGGGCTGGGGCCTCAGATGTAG
  AGGAGGAGGAGGGCCCGCTGGGGGGGGGTTCTGGACTCCAAGAACCCCTACAACTTGGAGAGTTGGACAT
  CACCTCTGATGAATTCATCTTGGATGAAGTGGATGTTCACATCCAGGCAAACCTGGAGGATGAGTTAGTG
  AAGGAAGCTCTCAAAACGGGTGTGGATCTTCGACACTATTCAAAGCAGGTGGAGCTGGAGCTGCAGCAGA
  TTGAGCAGAAGTCAATCCGGGACTATATCCAAGAGAGTGAAAACATAGCGTCTCTGCACAATCAGATCAC
  GGCCTGTGACGCCGTCCTGGAGCGCATGGAACAGATGCTGGGAGCTTTTCAGAGCGATCTCAGCTCCATC
  AGCTCTGAGATCCGAACCCTGCAGGAGCAGTCGGGGGCCATGAACATCCGACTTCGTAACCGACAGGCAG
  TTCGGGGGAAACTTGGGGAACTTGTGGATGGGCTGGTGGTGCCCTCTGCTCTGGTCACAGCAATTCTGGA
  AGCTCCAGTGACCGAGCCCAGGTTCCTGGAGCAGCTACAGGAGCTGGATGCCAAGGCAGCCGCGGTGAGA
  GAGCAGGAGGCTATGGGCACCGCTGCCTGTGCTGACGTCAGAGGCGTGCTGGACCGGCTCCGGGTCAAGG
  CAGTGACGAAGATCCGGGAGTTTATTCTCCAGAAGATCTACTCCTTCAGAAAGCCCATGACCAACTACCA
  GATCCCCCAGGCGGCCCTGCTGAAGTACAGGTTTTTCTATCAGTTCCTGCTGGGTAATGAGCGTGCTACA
  GCCAAGGAGATCAGGGATGAGTACGTAGAGACGCTGAGCAAGATCTACCTGTCCTACTACCGATCCTATG
  TGGGGCGGCTCATGAAAGTGCAGTACGAGGAAGTTGCTGAGAAAGACGACCTAATGGGTGTTGAAGACAC
  AGCAAAGAAAGGCTTCTTCTCGAAGCCGTCCCTCCGAAGCAGGAACACCATCTTTACCCTTGGCACTCGT
  GGTACTGTCATCTCACCGGCCGAGCTGGAGGCCCCCATCCTAGTGCCCCATACTGCCCAACGTGGAGAGC
  AGAGGTATCCATTCGAAGCGCTCTTCCGCAGCCAGCACTATGCCCTCCTCGACAATTCTTGCCGTGAATA
  TCTTTTCATCTGTGAATTTTTCATCGTGTCTGGCCCGGCTGCACATGACCTGTTCCACGCCGTCATGGGC
  CGCACGCTCTCCATGACGCTGAAACACCTGGAGTCCTACCTGGCTGACTGCTACGATGCCATTGCTGTTT
  TTCTCTGTATCCACATTGTTCTCCGGTTCCGCAACATTGCAGCGAAGAGGGACGTCCCTGCCCTGGACAG
  GTACTGGGAGCAGGTGCTTGCCTTGCTGTGGCCTCGGTTTGAGCTGATCCTGGAGATGAATGTCCAGAGT
  GTCCGCAGCACTGACCCCCAGCGCCTTGGGGGACTGGACACTCGGCCCCACTATATCACACGCCGCTATG
  CTGAGTTCTCCTCTGCACTTGTCAGCATCAACCAGACGATCCCCAATGAACGCACGCTGCAGCTCCTGGG
  ACAGCTCCAGGTGGAGGTGGAGAATTTTGTCCTCCGAGTGGCTGCAGAGTTCTCCTCCAGGAAGGAGCAG
  CTTGTGTTTCTGATCAACAACTATGACATGATGCTCGGGGTGCTGATGGAGCGGGCTGCTGATGACAGCA
  AAGAGGTGGAAAGTTTCCAGCAGCTGCTCAATGCTCGGACACAGGAGTTCATTGAGGAGCTGCTGTCTCC
  CCCCTTCGGGGGTCTGGTGGCATTCGTGAAGGAGGCTGAAGCCTTGATTGAGCGTGGGCAGGCTGAGCGG
  CTCCGAGGGGAGGAAGCCCGAGTCACTCAGCTGATCCGTGGCTTTGGTAGTTCCTGGAAGGCATCAGTGG
  AGTCCCTGAGTCAGGATGTAATGCGAAGTTTTACCAACTTCCGAAATGGAACCAGCATCATCCAGGGGGC
  GCTGACCCAGCTGATCCAGCTCTACCATCGCTTCCACCGGGTGCTGTCTCAGCCCCAGCTCCGCGCACTC
  CCGGCCAGGGCCGAGCTCATCAACATCCATCACCTCATGGTGGAGCTGAAGAAACACAAGCCGAACTTCT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172620
• Insert Size: 2172 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172620.3, NP_766208.2
• RefSeq Size: 2766 bp
• RefSeq ORF: 2172 bp
• Locus ID: 224705
• UniProt ID: Q8C754
• Cytogenetics: 17 B1
• Gene Summary: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function]