Tbc1d25 (NM_001166437) Mouse Untagged Clone

CAT#: MC221120

Tbc1d25 (untagged) - Mouse TBC1 domain family, member 25 (Tbc1d25), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbc1d25
• Synonyms: 6330576A08Rik; DXHXS7927e; Oatl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221120 representing NM_001166437
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCGGAAGTAAACTGCCAAAACAACGGCGCCACGGACCGGGCCAGGCAGGACCTGGACAGGCACT
  TACACACAGGCAGTAAAAAAGACGACAGCGGCGGGATGGCAACCACCTCCGCGGCCTCGGACTCGGCGTG
  CTCGGCAGCGCCCCCGCCCGTCGGAGGTGCCCAGGCGGCCGCGGCGGTTGAGGAGGAGGAGCGTGAGGTG
  GTACGGGTCCGAGTCAAGAAATGTGAGAGCTTCTTGTCCCCTGAGTTCCGCTCTTTTGCGGTGGATCCTC
  AGATCACCTCGCTGGACGTGTTGCAGCACATCCTCATCCGAGCCTTTGACTTAAATGGGAAGAAGAACTT
  TGGCATCAGCTACCTGGCTCGAGACCGGCTAGGACAGGAAACTTTCCTCTCCCTCCTTTCTGATTGGGAC
  CTCAGCACAGCTTTCGCCACTGCCTCCAAACCTTATCTGCAGTTGCGTGTAGACATTCGGCCCTCAGAGG
  ACAGCCCATTGCTGGAAGACTGGGACATAATTAGCCCCAAAGATGTCATTGGATCTGATGTGCTACTGGC
  TGAGAAGCGATCATCACTGACGACAGCTGCCTTGCCTTTTACACAGTCCATCCTCTCTCAGGTGGGTCGT
  ACACTGTCTAAGGTCCAGCAGGTGCTGAGTTGGTCTTATGGGGAAGATGTGAAGCCTTTTAAGCCCCCCC
  TGAGTGATGCTGAGTTCCACACGTACCTGAATCATGAAGGTCAGCTCTCCCGGCCTGAGGAGTTGCGTTT
  GAGGATCTACCACGGCGGTGTGGAGCCTTCACTTCGAAAAGTAGTATGGCGGTACCTCCTTAATGTGTAC
  CCAGATGGGCTGACTGGCCGTGAGCGGATGGACTACATGAAACGTAAGAGCCGAGAGTACGAGCAGCTCA
  AAAGTGAGTGGGCCCAGCGAGTGAACCCTGAGGACCTGGAATTCATCCGCAGCACAGTCCTCAAGGATGT
  GCTCCGCACAGACCGAGCCCATCCCTACTACGCAGGGCCTGAGGATGGCCCACACCTACGGGCCCTACAC
  GACTTGCTCACCACCTATGCCGTTACCCATCCACAGGTATCATACTGCCAGGGGATGAGTGACCTGGCCT
  CACCAATTCTTGCTGTCATGGACCATGAAGGCCATGCCTTTGTATGCTTCTGTGGCATCATGAAGCGCTT
  AGCTGCCAACTTCCACCCTGATGGCCGTGCCATGGCCACCAAGTTTGCCCACCTCAAGCTGCTATTGAGA
  CATGCTGACCCAGACTTCTATCAGTACCTGCAAGAAGCAGGTGCCGATGACCTCTTTTTCTGTTACCGCT
  GGCTGCTGCTGGAGCTCAAGCGTGAATTCGCCTTCGATGATGCTCTCCGAATGCTAGAAGTCACCTGGAG
  TTCACTGCCCCCTGACCCTCCTGAACACGAAGTGGAGCTCGTCGGACCTCCCAGCCAAGTAGCAGACACT
  GGCTTTGGTAGCCACAGGGGACGGCCAGTACGTCAGAGGCACATGCTGAGGCCTGCTGGTGGAGGAGGCG
  GTGCTTTTGAAGATGCTGTTGTCCACTTAGCTGCATCTAGCCAGGGACCTAGTGGTGGGGGGCGTCTCCT
  AAGACAAGCCAGTCTGGATGGTCTCCAACAACTCAGGGATAACATGGGCCTCAGGAAGGACCATCTGGTC
  CAACTATCCCACCCAGCTACCCTCATTAGCTCTAAGTCCCTCTCTGAGCCCTTGCTGAATTCCCCAGATC
  CACTCCTCTCCACCTCTTCTCGGCCCGATTCCCCATCTTCGTCATCTCCTCCATCTACCCAGGAGGCTTC
  TCCTTCTGGTGACATAGCTGTAGGATCCCCCTTAATGCAAGAGGTAGGTTCTCCCAGAGACCCTGGGAAG
  CCTGTACCACCTCCACCTCCAATGGGCCTTCCCCCACCCCAGGAGTTTGGCCGAGGGAACCCATTCATGC
  TCTTCCTCTGCTTGGCTATCCTGCTGGAGCATCGTGACCACATCATGCGCAATGGGCTGGATTACAATGA
  GCTAGCCATGCATTTTGACCGCCTGGTTCGTAAACACCATCTAGGGCGTGTCTTGCGTAGGGCCAAAGCA
  CTCTTTGCTGATTACCTACAGTCTGAAGTGTGGGACTCTGAGGAGGGGGCTGAGGCCACAGCTCCATCTT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166437
• Insert Size: 2172 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166437.1, NP_001159909.1
• RefSeq Size: 2452 bp
• RefSeq ORF: 2172 bp
• Locus ID: 209815
• UniProt ID: A1A5B6
• Cytogenetics: X 3.69 cM
• Gene Summary: Acts as a GTPase-activating protein specific for RAB33B. Involved in the regulation of autophagosome maturation, the process in which autophagosomes fuse with endosomes and lysosomes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks a portion of the 5' UTR and 5' coding region and initiates translation at alternate upstream start site, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus when it is compared to isoform 1.