Ccnt2 (NM_028399) Mouse Untagged Clone

CAT#: MC221106

Ccnt2 (untagged) - Mouse cyclin T2 (Ccnt2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccnt2
• Synonyms: 2900041I18Rik; C81304; CycT2; CycT2a; CycT2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221106 representing NM_028399
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGGGCCGTGGAGCTTCTTCTCGCTGGTTCTTTACTCGGGAACAGCTGGAGAACACGCCGAGCC
  GCCGCTGCGGAGTGGAAGCGGATGAAGAGCTGTCGCATCGCCAGCAGGCGGCCAACCTCATCCAGGACAT
  GGGGCAACGTCTCAATGTCTCTCAGCTTACAATAAACACTGCGATTGTTTATATGCACAGGTTTTATATG
  CACCATTCCTTCACCAAATTCAACAGAAATATAATATCACCTACTGCATTGTTTTTGGCTGCAAAAGTGG
  AAGAGCAGGCTCGAAAGCTTGAACATGTTATCAAAGTAGCACACGCTTGTCTTCATCCCCTAGAGCCACT
  CCTGGACACAAAATGTGATGCTTATCTTCAGCAGACTCAAGAACTGGTTTTACTTGAAACCATAATGCTA
  CAAACACTGGGTTTTGAGATCACCATTGAACACCCACACACAGATGTGGTGAAATGCACCCAGTTAGTAA
  GAGCAAGCAAGGATTTGGCACAGACATCCTATTTCATGGCTACCAACAGTTTGCATCTTACGACCTTCTG
  TCTTCAGTACAAACCCACAGTGATAGCATGTGTATGCATTCATTTGGCTTGCAAATGGTCCAATTGGGAG
  ATTCCTGTGTCAACAGATGGAAAGCACTGGTGGGAGTATGTGGACCCCACGGTTACCCTAGAGCTGCTAG
  ATGAACTAACACATGAGTTTCTACAAATATTGGAGAAAACGCCTAGTAGGTTGAAGAGGATTCGAAACTG
  GAGGGCTATGGCTAAGAAACCAAAAGTAGATGGACAAGTATCAGAGACACCACTGCTTGGTTCATCTTTG
  GTCCAGAATTCTATTTTAGTAGATAGTGTCACTGGTGTACCTGCCAACCCAAGCTTTCAGAAACCATCAA
  CATCAACGTTCCCTGCTCCAATACCTCTAAATTCAGGAAGTACTTCTGTTCAAGATAGCCGTGCATCTGA
  TAATTTATCAGTGCTAGCAGCAGGAATGCCCAGTACCTCATACAGTTTGTCATCACACCAAGAGTGGCCC
  CAGCATCCAGATTCAGCCAGGACAGACCCAGTGTACACACAGAAACAGGAGGCCACTCTCTCTGGGAGCC
  AGTACATAAGCTTCCAGCAGGGACCTTCAATGGCACTGCATTCTGGATTACATCACAGGCCGGACAAAGT
  TGCTGATCATTCATCAGCTAAGCAAGAATATACTCACAAAGCTGGGAGCAGTAAACACCATGGTCCCATA
  CCTGCTACTCCTGGAATGCTTCCTCAGAAAATGTCTTTAGATAAGTACAGAGAGAAGCGGAAGCTGGAAA
  CCCTTGACGTGGACACAAGGGATCACTATCTAGCTGCCCATGCAGAACAGCAGCACAAGCATGGGCCGGC
  TCAGGCAGTCACGGGCACTTCTGTCACTTCTCCCATCAAGATGAAGCTCCCCCTCACCAACTCGGACAGG
  CCTGAGAAACACGTGGCAGAGAAAAAGGAGAGGAGTGGATCACTGAAGCTCCGCATTCCCATCCCCCCGC
  CTGACAAAGGTCCCAGCAAAGAGGAGCTGAAGATGAAAATCAAAGTGGCATCCTCGGAAAGACACAGCTC
  TTCTGATGAAGGCAGTGGGAAGAGCAAGCACTCAAGCCCACACATCAGCAGAGACCACAAGGAGAAGCAC
  AAGGAGCACCCTGCCAACCGCCACCACAGCAGCCACAAGTATTTGCACATGCACAGTGGAGGCAGTAAGC
  ACACAGCGGATGGAATGCCTCCCACGGTGCTCAGGAGTCCTGTGGGCCTGGGCCCTGAAGGCGTCTCCTC
  CGCCTCCAGTGCAAGGAAGAAGCTGCATAGCAGCGAAGCCTCCCACAACCACCACTCCAAAATGAGCAAA
  AGTTCCAAAAGTGCAGGTAGTTCATCTAGTTCTTCCTCTGTTAAGCAGTATCTATCCTCTCACAGCTCTG
  TTTTTAACCATCCCTTACCCCCTCCTCCCCCTGTCACATACCAGGTGGGCTACGGACATCTCAGCACCCT
  CGTGAAACTGGACAAGAAACCAGTGGAGCCCCACGGTCCTGAGGCCAATCACGAGTACAGTACAAGCAGC
  CAGCATATGGACTACAAAGACACATTCGACATGCTGGACTCGCTGCTAAGTGCCCAAGGAATGAATATGT
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028399
• Insert Size: 2172 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028399.1, NP_082675.1
• RefSeq Size: 3514 bp
• RefSeq ORF: 2172 bp
• Locus ID: 72949
• UniProt ID: Q7TQK0
• Cytogenetics: 1 E3
• Gene Summary: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). The activity of this complex is regulated by binding with 7SK snRNA (By similarity). Plays a role during muscle differentiation; P-TEFB complex interacts with MYOD1; this tripartite complex promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation and binds the chromatin of promoters and enhancers of muscle-specific genes; this event correlates with hyperphosphorylation of the CTD domain of RNA pol II (PubMed:16245309, PubMed:23060074, PubMed:12037670). In addition, enhances MYOD1-dependent transcription through interaction with PKN1 (By similarity). Involved in early embryo development (PubMed:19364821).[UniProtKB/Swiss-Prot Function]