Tbx3 (NM_198052) Mouse Untagged Clone

CAT#: MC221091

Tbx3 (untagged) - Mouse T-box 3 (Tbx3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbx3
• Synonyms: D5Ertd189e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_198052, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCTCTCCATGAGAGATCCGGTTATCCCTGGGACAAGCATGGCCTATCATCCCTTCCTACCTCACC
  GGGCGCCGGACTTCGCCATGAGCGCGGTACTGGGCCACCAGCCGCCTTTCTTCCCCGCGCTAACGCTGCC
  TCCCAACGGCGCCGCGGCGCTCTCGCTCCCCGGAGCTCTGGCCAAGCCCATCATGGATCAGTTAGTGGGG
  GCTGCTGAGACCGGCATCCCTTTCTCATCCCTGGGACCCCAGGCACATCTGAGGCCTCTGAAGACCATGG
  AACCCGAAGAAGACGTAGAAGATGACCCTAAGGTGCACCTGGAGGCCAAGGAACTTTGGGACCAGTTTCA
  CAAGCGGGGTACAGAGATGGTCATCACGAAGTCAGGAAGGCGAATGTTCCCTCCGTTTAAAGTGAGGTGC
  TCTGGACTGGATAAAAAGGCCAAGTATATTTTATTGATGGACATTATAGCTGCTGACGACTGTCGATATA
  AATTTCACAACTCTCGGTGGATGGTGGCCGGTAAGGCAGACCCCGAAATGCCAAAGAGAATGTATATACA
  CCCGGACAGCCCCGCTACGGGGGAGCAATGGATGTCCAAAGTCGTCACTTTCCACAAACTGAAACTCACC
  AACAACATATCGGATAAACACGGATTTACTATACTAAACTCTATGCATAAGTACCAGCCGCGGTTCCACA
  TCGTCAGAGCCAACGATATCCTGAAACTGCCTTACAGTACTTTTCGAACCTACCTGTTCCCGGAAACAGA
  ATTCATCGCCGTTACTGCCTATCAGAATGACAAGATAACTCAGTTAAAAATAGACAACAATCCCTTTGCA
  AAGGGTTTTCGAGACACTGGCAATGGCAGGAGAGAAAAAAGAAAGCAGCTCACACTGCAGTCCATGAGAG
  TGTTTGAGGAGAGGCATAAGAAGGAGACTTCGGATGAGTCCTCCAGCGAGCAGGCAGCCTTCAACTGCTT
  TGCCCAGGCATCCTCTCCTGCTGTCTCCATCGTGGGGACATCCAACCTCAAAGATCTGTGTCCCAGCGAA
  GCCGAGAGTGACGCCGAGGCTGAGAGCAAGGAGGAGCACGGCCCTGAGGCCTGCGACGCGGCCAAGATTT
  CCACCACTACGGCCGAGGAGCCAGGCCGCGACAAGGGCAGCCCAGCAACCAGGGCGCAGCTGTTCCCCGC
  GGAGCCCAGCCGGGCCCGCGACACCGCGCGTCTGGACAAGGCATCGCCAGACTCGCGCCATAGCCCGGCC
  ACCATCTCGTCCAGCACGCGAGTTCCGGGAGCTGATGAGCGCAGGAGTCCCGGGCGCGAGGGTCCAGTCG
  CCACCAAGGTGGATGAGGCGCGCGCGATTCCTGCCAAGGACGCCTTCGCTCCACTGTCGGTGCAGACAGA
  CGCCACCGCGCACCTAGCGCAGGGGCCCCTCCCGGGCTTGGGTTTCGCCCCAGGCCTTGCGGGCCAGCAG
  TTCTTCAACGGGCACCCGCTCTTCCTGCACCCGGGCCAGTTCGCCATGGGCGGCGCCTTCTCTAGCATGG
  CTGCAGGCATGGGGCCCCTGCTGGCCACAGTATCCGGAGCATCCACCGGCGTCTCAGGCCTAGAATCCAC
  AGCCATGGCCTCGGCTGCCGCAGCGCAGGGACTGTCTGGGGCGTCGGCTGCCACCCTGCCCTTCCACCTC
  CAACAACACGTTCTGGCCTCCCAGGGCTTGGCTATGTCGCCTTTCGGGAGCCTGTTTCCTTACCCCTACA
  CATACATGGCTGCAGCAGCTGCAGCCTCCACCGCGGCCGCCTCCAGCTCCGTGCACCGCCACCCGTTCCT
  CAATTTGAACAGCATGCGCCCCAGGCTGCGTTACAGCCCCTATTCCATCCCCGTCCCAGTGCCGGATAGC
  AGCAGTTTGCTGGCCACAGCTTTACCATCAATGGCTTCCGCCGCGGGGCCCCTAGACGGCAAAGCGGCCG
  CCCTGGCAGCCAGCCCAGCCTCGGTGGCTGTGGACTCGGGGTCGGAACTGAACAGCCGCTCGTCCACGCT
  GTCCTCTGGCTCAGTGTCCTTGTCACCCAAACTCTGCTCCGAGAAGGAGGCGGCTACCAGCGAACTGCAG
  AGTATCCAGCGGCTGGTCAGTGGCTTGGAAGCCAAGCCAGACAGGTCTTGCAGCGGGTCCCCTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_198052
• Insert Size: 2166 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC096551, AAH96551
• RefSeq Size: 4087 bp
• RefSeq ORF: 2166 bp
• Locus ID: 21386
• Cytogenetics: 5 60.34 cM
• Gene Summary: Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.