Arhgef16 (NM_001112744) Mouse Untagged Clone

CAT#: MC221044

Arhgef16 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 16 (Arhgef16), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221044 representing NM_001112744
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGAGACATTCTGACAGCTCTCTGGATGAGAAACTACTGGAGTATCGCTTCCACGCAGAGCTGC
  GCCTCGATGCCAACGGGAACCCTATCCCTGGACTCCCCATGGTCCGGAGCTCCTTGAAAGCCAGAGCCAA
  TGCTGCCTTTGAGCCAGATGCCTCAGAACCCCTGCCGCCACCTCCGTCACCAGAAGACGAAGAGCCTCCT
  CGGCCCATCGTCCTTAGCACGCAAAGCCCCGCGGCCCTCAAGATGGGCACGCAGCAGCTCATCCCCAAAA
  GTCTGGCTGTGGCCAGCAAGGCCAAGACCAAGTCCCCAGCCCGCCACCAGAGCTTTGGGGCGGCTGTGCT
  CAGCAAGGAGGCTGCCAGGCGGAATCCTCAGCTCTTCTCAGCCCCCAGCTTCTCGTTGGATGACATGGAT
  ATGGACATGGTTACTACCGGGAACCTGAGGCGCAACCTGAGGAACCAGTCCTACAGGGCAGCCATGAAGG
  GGCCGGGGCCACCCAGTAGCAAGGGGGACTCAGTCCAGCTTGGCCCCAAGCTCCAGGCTCTCGCTGAGGA
  GGCTGCTCAGCCTCCCTCCCGGTATCCAGCCAAAAATAAGAAAACTCTCGGTCGGAAACGTGCGCACAAG
  GGCTCTTTCAAAGATGACCCCCAGCTGTATCAGGAGATCCGAGAGCGGGGTTTGAACACCAGTCACGAGT
  CCGATGATGACATTCTTGATGAGCCCTCCGGCCCAGTGGGAACTCAGAGAGCGGACACCACCATCGTGGT
  CAAGAGCTATCGGCCTGCTCAGCTCACCTGGAGCCAGCTCCCAGAGGTGCTGGAGTCAGGTGTCCTGGAC
  ACGCTGTCAACGGAAGAACGGAAGAGGCAGGAGGCCATCTTCGAGATCCTCACTTCTGAGTTCTCGTATC
  TGCACAGCCTGAGCATCCTGGTGACTGAGTTCCTGCAGTCCCGGGAGCTGAGGGCCACTATGACCCAGAC
  GGAACACCACCACCTCTTCTCCAACATCTTGGATGTGATGTCTGCCAGTCAGAAGTTCTTTGAAGCCCTG
  GAGCAGCGGCACAAAGCTCAGGTGTGCGTGGAGGACATCAGCGACATCTTGGAGGACCATGCTCAGCATC
  ATTTCCACCCTTACATCGCCTACTGCTCCAACGAGGTCTATCAACAGCGCACCCTGCAGAAGCTGTCGAA
  CAGCAACGCAGCCTTCCGGGACGTTCTGAAGGAGATCGAGAAGCGGCCTGCGTGTGGGGGCCTGCCCATG
  ATCTCTTTCCTGATTCTCCCCATGCAGAGAGTGACCCGGCTGCCGCTGCTGACAGATACACTCTGCCTGA
  AGACGCAAGGCCATCCCGAACGGTATAAAGCTGCCAGCCAAGCCCTGAAGGCCATCAGCAAGCTGGTGAA
  GCAGTGTAACGAGGGAGCTCACAAGATGGAGCGCACAGAGCAGATATACACACTGAACATGCAGCTGGAC
  TTCGGCAAGGTCAAGTCCCTCCCGCTCATCTCCGCCTCTCGCTGGCTGCTGAAGCGTGGGGAGCTCTTCC
  TCTTGGAGGAATCCAGTATCTTTAGGAAAATCGCCAGCCGACCCACGTGTTATCTGTTCCTGTTCAATGA
  TGTCCTGGTTGTCACCAAGAAGAAAAGTGAGGAAAGCTACCTGGTCCAAGACTACGCGCAGCTAGACCAC
  GTACAGGTCAGGAAACTGGAACCCTCGGAGCCCTTGCTGCCAGGAGGCAGCAGCCGCAGCTCCTCCGTGC
  CCTACCCATTCCAGGTGAACCTGCTACATAACAGCGAGGGCCGCCAGGAGCAGATACTGCTGTCGTCCGA
  CTCTGCGAGTGACCGGGCCCGGTGGATCACAGCACTCACCTACAAGGAGAGACAGTGGCAGGGCATCACC
  AACAAAGGAGAACTCCCCCAGGTAGAGGTCACCAAGGCGTACTTTGCCAAGCAGGCTGATGAGATCACAC
  TGCAACAGGCTGACATCGTCCTGGTCCTGCAGGAAGAGGACGGTTGGCTACACGGGGAGCGGCTTCGGGA
  CGGAGAGACCGGCTGGTTTCCCGAGAGCTTTGCTCACTCCATCACCAGCCGTGTGGCCGTGGAAGGCAAC
  GTGCGCAGGATGGAGCGGCTGCGGGTGGAGACGGATGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001112744
• Insert Size: 2142 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001112744.1, NP_001106215.1
• RefSeq Size: 2717 bp
• RefSeq ORF: 2142 bp
• Locus ID: 230972
• UniProt ID: Q3U5C8
• Cytogenetics: 4 E2
• Gene Summary: Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 (By similarity).[UniProtKB/Swiss-Prot Function]