Rsph4a (NM_001162957) Mouse Untagged Clone

CAT#: MC221036

Rsph4a (untagged) - Mouse radial spoke head 4 homolog A (Chlamydomonas) (Rsph4a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rsph4a
• Synonyms: A230081C05; Rshl3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221036 representing NM_001162957
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAACTCTACCTCTCTGAAACAAGAAAAGGAAAATCAAGAGCCAGGGGAAGCAGAGAGATTATGGC
  AAGGAGAGTCAGACGTTTCTCCCCAGGAGCCTGGTCCCCCATCACCTGAGTACCGGGAAGAGGAGCAAAG
  GACAGACACCGAACCCGCACCCAGAATGAGCCCCTCTTGGAGCCACCAGTCTAGAGTCAGCCTGTCTACG
  GGAGACCTCACCGCTGGACCAGAAGTGTCATCCTCCCCTCCCCCTCCTCCTCTGCAGTTTCATTCCACTC
  CTCTCAACACTGAAACTACTCAGGATCCTGTAGCTGCATCACCCACAGAGAAGACCGCTAATGGAATCGC
  TGACACCGGGACACCTTATTCTGATCCTTGGGAATCATCTTCTGCTGCAAAGCAATCAACTTCTCACTAC
  ACAAGCCACGCCGAGGAAAGTACTTTCCCGCAGTCTCAGACACCCCAACCTGACCTCTGTGGGCTAAGGG
  ATGCCAGCAGGAACAAGTCCAAGCACAAAGGGCTGAGATTTGACCTTTTACAAGAGGAAGGCTCAGACAG
  TAACTGTGACCCAGACCAGCCTGAGGTTGGAGCCAGTGAAGCAGCCCAGAGTATGCTTGAGGTGGCCATT
  CAGAATGCAAAAGCCTACCTGCTGAGTACCAGCAGCAAGTCTGGCTTAAATCTATATGACCATCTTTCTA
  AAGTGTTGACCAAGATACTAGATGAGCGTCCTGCAGATGCTGTTGACATCATTGAAAATATAAGCCAAGA
  TGTGAAAATGGCACATTTTAATAAAAAATTAGATACACTCCACAATGAATATGAGATGCTTCCAGCATAT
  GAAATAGCAGAGACACAAAAGGCACTTTTTCTCCAGGGACATTTGGAAGGTGCTGATTCAGAACTGGAGG
  AGGAAATGGCAGAAAGTTCTCTGCCCAATGTGATGGAATCAGCTTATTATTTTGAACAAGCTGGAGTTGG
  TTTGGGGACAGATGAGACTTACCGAGTATTCCTCGCCCTCAAGCAACTTACTGATACACACCCAATCCAA
  AGATGTCGCTTCTGGGGCAAGATCTTGGGTCTGGAAATGAACTATATTGTTGCTGAAGTGGAATTTCGTG
  ATGGTGAAGATGAAGAGGAGGTGGAAGAAGAAGGGATAGCTGAGGAGAGGGACAATGGAGGAAGTGAGGC
  CGGTGAAGAGGAGGAGGAGGAATTACCGAAGTCCTTGTATAAGGCCCCACAGGTTATACCTAAGGAAGAA
  AGTAGAACGGGTGCCAACAAGTATGTCTATTTTGTTTGCAATGTGCCTGGAAGGCCATGGGTAAGGTTAC
  CATCAGTTACACCTGCACAAATTGTCACTGCAAGAAAAATCAAGAAATTTTTCACTGGGCGACTAGATGC
  AGCTGTCATAAGCTACCCACCCTTCCCAGGAAATGAGAGCAATTACTTAAGAGCACAAATTGCCCGCATT
  TCAGCAGGGACCCATGTCAGTCCTCTAGGATTCTATCAGTTTGGTGAAGAGGAAGGAGAGGAAGAAGAGG
  TGGAAGGTGGTCGAGACAGCTATGAGGAAAACCCTGACTTTGAAGGCATCCAAGTGATTGACTTGGTGGA
  GTCTCTATCCAATTGGGTTCATCACGTGCAGTATATTCTTCCTCAGGGTCGCTGTAATTGGTTCAACCCT
  ATACAAAAAGATGAAGATGAAGAAGAGGAAGAGGAAGAAGATGAAGAAAAGGGGGAAGAACCTGACTACA
  TAGAGCAGGAAGTCGGACCTCCTCTTTTGACACCAATATCTGAAGATTTGGGGATTCAGAATATACCCTC
  TTGGACAACACAGCTGTCTTCAAATCTCATTCCACAGTATGCCATTGCTGTCCTTCGCTCCAATCTTTGG
  CCTGGAGCATATGCTTTTTCCAATGGCAAGAAGTTTGAAAACTTCTACATAGGCTGGGGTCATAAGTACT
  GTGTGGAGAATTACACACCCCCAAGCCCACCACCAGTTTATCAAGAATATCCCAGTGGACCAGAAATTAC
  AGAAATGAATGATCCCAGTGTGGAGGAGGAACAGGCTTTCAGGATGACACAAGAGCCAGTTGCACTTTCA
  ACTGAGGAGAACGAAGGAACTGAAGATGAAGATGAGGACGATGAGGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001162957
• Insert Size: 2151 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162957.1, NP_001156429.1
• RefSeq Size: 3650 bp
• RefSeq ORF: 2151 bp
• Locus ID: 212892
• UniProt ID: Q8BYM7
• Cytogenetics: 10 B1
• Gene Summary: Probable component of the axonemal radial spoke head (By similarity). Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules.[UniProtKB/Swiss-Prot Function]