Fancb (NM_175027) Mouse Untagged Clone

CAT#: MC220921

Fancb (untagged) - Mouse Fanconi anemia, complementation group B (Fancb), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fancb
• Synonyms: BC022692
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220921 representing NM_175027
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATTTAATGAGCAAGCAAAGTTCTTGTGCTATCATGGGGAAATCCTTGTCTTTCAGTTGTCCAGAG
  GAGAGCGTGCAGATTTAGAAATGCCTACAGATCCACTGGTCTTAGGTGTCAAAAGGATGATGTTTGACAG
  AGAAACTAGTACATTCCTTCTGATATCCACTGTTTTTTTAAACATCAACGAAAAAGATTCTCATTTAAAA
  ATTCTGTGTTGTAACTGTGTGTCCGATTTGAGAACTAGAATTAACCTTCCTTGCGTTTTGATACAATGTA
  GAAAGTACAATAGTGAAGCTTTCAAATATTGTATCCTGCTTCTTCACAATCTCAACAGAGTGGAGAGACT
  TTTGAGTTTTGAACTAAACCATGCATTGGATGAGAACACAAAGATCTTTGACGGCCCTATAGTTTTCTGG
  CAGTATCTCAACCAGTTCTTCTATATCTCTTCTGCAATTGGGAAAGTTACTACTATATCACTTATGCTTT
  CCTCTATTGAGTGGATCGGTGAGATTGAAAACTTTGGCCTAGGTTTCTTGGGGCTAGCAGAACCATCTGA
  GGATAAATGTACCCAGAAGCTTTCAGAATCAGACTATGAATTTTCCAATTCCAGCCTTTGCGCATATGCT
  CTTAAAAGTCAAGAAATGTTAAGCAATGGTTACCTGATCCCTCTTGCTTACAGTACTATGGTAACACACG
  TGCATGTCTGGGCGGCGGAGATGGTTGACCACCAGTTAAGAACATCTCTGATTGCCCTGACTCGAAAGAA
  TCAGCTCATTTTGTTTCAAAATGGCATTCCTGTAAGAGCATGTCAGCTTCCATTTCCAGGTCCCCGTTCA
  GTTCAGATTCTAGATGCAGGGAAAAGAAACCGATTTTTCATCGTGTCATTCCCGTCCAAGGCTTGTGCTG
  TTTCAGAAAAGAAATTTAAGGTTGTTGCTAAGTGGGAACAACTTAGCTTAGTACTGGTGAATGACTTTGC
  TGGAGTTGGAACTGAACAAGTACTGGTCGTTTTTGAGGACTCTCTGGATGCAGATCAGCTGACCTCATTT
  ACAGTAACAGATTTTGTCAAAATATGGTATTCAACCAAACCACTGGATTGCTGTGAAGACCCTTTGGCTG
  AAGAGGAGCATGAAAATTATTATTTGGTGCTTCCAGCACTGGAAGGCCAATTAGACAATTCTTTTATTTT
  TCTTAACAAAATCCAGCAGCATATCTCTTTTAAGGATAAATTTATTGCAAAGTCTTGGAAGGCTCTTTTA
  AACGCAGTTTATGGGAAAGGTGACAGTCTGCCAAGTGATGAAATGGTTAAGTTAGACCAGGACGGTCTTG
  TTCCTTTCTGTGATGAAGGTGAAGACAGTGTCCCTACCCCTGAGGAAAACTTACCAGACAATTTTCCAGA
  GCCAGAACATATTGTAGAGCAGACATGGTGCCATGTACTAGATGATGACTTAGTTGTGGGCGCAAAAGTC
  ACATCTTTGAAGGAATCTAATGAGATGACTTTATCACTGATAATGAATCAAGGCAACAGGTCCAGTTTTC
  ATCTTATGAAGTGTCACAGTCAGGTATGGCTCTTGAAACACATGAAATGTGAAAGAATTCAAGAATGTAC
  AGAAATATACCTTTATAAAAAGCTACGAAATTGTGGAGCACTCTTCTCCTGGGAGCAGAGAACAGCATCT
  GAAGGAATTTTAACAATATATTGCAGGAGCCAAGGAGTTTTGTTCCAGTGTCTCGACCATCTCATCAAAG
  TTCTCCCTGAAATATGTTCCTTCAAATATCTGAAGGTCGAAAATGAGGATTTCTTAGTTGATCATTTGTC
  ATCCACTTTGGAGGCAGAACTGGTTACCTTTTGTTCAGTTTCTACTTCTGCCTTCGAGTATGTTAGAGGT
  GGATATAACTGTAGAATAAGGAGAACAGATAATAGGGCCATGACATTTTTAGGGAGAAGGGCCAAAATCC
  GTCAGTCCAAGCGCAAAGTTCAGAGAGAAAGGATACTGAAGCACCTTAATATGACAGTAAATGGCAGCAG
  CTATGCGGAGATGACTTTGGCGTTGGCTGAGATTCAGTTGAAATCAGACCTCATTGTAAAGACACTGGCC
  AATTTTGTAATTGCACTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175027
• Insert Size: 2121 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175027.4, NP_778192.3
• RefSeq Size: 2543 bp
• RefSeq ORF: 2121 bp
• Locus ID: 237211
• UniProt ID: Q5XJY6
• Cytogenetics: X F5
• Gene Summary: DNA repair protein required for FANCD2 ubiquitination.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame segment in the 3' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.