Unc5b (BC057560) Mouse Untagged Clone

CAT#: MC220874

Unc5b (untagged) - Mouse unc-5 homolog B (C. elegans) (cDNA clone MGC:66787 IMAGE:6417563), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Unc5b
• Synonyms: 6330415E02Rik; A630020F16; D10Bwg0792e; Unc5h2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057560
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGCCCGGAGCGGGGTGCGGAGCGCGCTGCTGCTGGCGCTGCTGCTTTGCTGGGATCCGACACCGA
  GCCTAGCAGGCGTTGACTCTGCTGGCCAGGTGCTCCCAGACTCCTACCCATCAGCCCCTGCGGAGCAGCT
  GCCGTACTTCCTATTGGAGCCACAGGACGCCTACATCGTAAAGAACAAGCCAGTGGAACTGCACTGCAGA
  GCCTTCCCTGCCACGCAGATCTACTTCAAGTGTAATGGCGAGTGGGTCAGCCAGAATGACCACGTCACAC
  AGGAGAGCCTGGATGAGGCCACAGGCTTGCGGGTGCGAGAGGTGCAGATCGAGGTGTCACGGCAGCAAGT
  GGAGGAACTCTTCGGGCTCGAGGACTACTGGTGCCAGTGCGTGGCCTGGAGCTCTTCGGGAACTACCAAG
  AGTCGCCGAGCCTACATCCGCATTGCCTACTTGCGCAAGAACTTTGACCAGGAGCCTCTGGCCAAGGAGG
  TACCCTTGGATCATGAGGTCCTTCTGCAGTGCCGCCCACCGGAGGGAGTGCCTGTGGCTGAGGTGGAATG
  GCTCAAGAATGAAGATGTCATTGACCCCGCTCAGGACACTAACTTCCTGCTCACCATTGACCACAACCTC
  ATCATCCGCCAGGCGCGCCTCTCAGACACGGCCAACTACACCTGTGTGGCCAAGAATATCGTGGCCAAGC
  GCCGGAGCACCACGGCCACAGTCATCGTCTATGTGAATGGAGGCTGGTCCAGCTGGGCAGAGTGGTCACC
  CTGTTCCAATCGCTGTGGCCGAGGCTGGCAGAAGCGTACTCGGACCTGCACCAATCCAGCCCCACTCAAT
  GGAGGCGCCTTCTGTGAGGGACAGGCCTTCCAGAAGACAGCTTGCACCACCGTGTGCCCAGTGGATGGAG
  CGTGGACCGAGTGGAGCAAGTGGTCTGCCTGCAGCACAGAGTGTGCGCACTGGCGCAGCCGCGAGTGCAT
  GGCACCGCCACCCCAGAACGGAGGCCGTGACTGCAGCGGGACGCTACTTGACTCCAAGAACTGCACTGAT
  GGGCTGTGCGTGCTGACCCTGGAGACATCGGGAGATGTGGCACTGTACGCAGGCCTTGTGGTGGCCGTCT
  TTGTGGTGGTAGCGGTTCTCATGGCCGTGGGAGTGATCGTATACCGGAGAAACTGCCGGGACTTCGACAC
  GGACATCACCGACTCCTCTGCGGCCCTCACTGGTGGCTTCCACCCTGTCAACTTCAAGACTGCAAGGCCC
  AACAACCCGCAGCTCCTGCACCCGTCCGCCCCTCCAGACCTAACGGCCAGTGCTGGCATCTACCGCGGGC
  CTGTGTATGCCCTGCAGGACTCCGCCGACAAGATCCCCATGACTAATTCGCCCCTGCTGGATCCCCTGCC
  CAGCCTCAAGATCAAGGTCTATAACTCCAGCACCATCGGTTCTGGGTCTGGCCTGGCTGATGGAGCCGAC
  CTGCTGGGTGTCCTCCCGCCGGGCACGTACCCAGGCGATTTCTCCCGGGACACCCATTTCCTGCACCTGC
  GCAGTGCCAGCCTTGGTTCCCAGCACCTCCTGGGCCTACCTCGGGACCCCAGCAGCAGTGTCAGCGGCAC
  CTTTGGTTGCCTGGGAGGAAGGCTGAGCCTCCCCGGCACAGGGGTCAGCCTGTTGGTACCAAATGGAGCC
  ATTCCCCAGGGCAAGTTCTATGACCTGTATCTACATATCAACAAGGCCGAAAGCACCCTCCCACTTTCAG
  AAGGTTCCCAGACAGTATTGAGCCCCTCGGTGACCTGTGGGCCCACAGGCCTACTCCTGTGCCGCCCTGT
  CGTCCTCACCGTGCCCCACTGTGCTGAAGTCATCGCTGGAGACTGGATCTTTCAGCTCAAGACCCAGGCC
  CATCAGGGCCACTGGGAGGAGGTGGTGACCTTGGATGAGGAGACCCTCAACACACCCTGCTACTGCCAGC
  TGGAGGCTAAGTCCTGCCACATCCTGCTGGACCAGCTGGGTACCTACGTATTCATGGGCGAGTCCTACTC
  TCGCTCTGCAGTCAAGCGGCTCCAGCTGGCCATCTTCGCCCCAGCCCTCTGCACCTCCCTGGAGTATAGC
  CTCAGGGTCTACTGTCTGGAGGACACACCTGTAGCACTGAAGGAGGTCCTGGAGCTGGAGAGGACTCTGG
  GTGGCTACTTGGTGGAGGAGCCCAAGCCTTTGCTCTTTAAGGACAGTTACCACAACCTACGCCTCTCCCT
  CCATGACATCCCCCATGCCCACTGGAGGAGCAAACTACTGGCCAAGTACCAGGAGATTCCCTTCTACCAC
  GTCTGGAATGGCAGCCAGAGAGCCCTGCACTGCACTTTCACCCTGGAGAGGCATAGCCTGGCCTCCACGG
  AGTTCACCTGTAAGGTCTGCGTGCGGCAGGTCGAAGGGGAAGGCCAGATTTTCCAGCTGCACACAACGTT
  GGCCGAGACGCCTGCTGGCTCCCTGGATGCTCTCTGCTCTGCCCCGGGCAATGCCATCACCACCCAGCTG
  GGACCCTATGCCTTCAAGATACCCCTGTCCATCCGCCAAAAGATCTGCAGCAGCCTGGACGCCCCCAACT
  CCCGGGGCAACGACTGGAGGCTGTTGGCGCAGAAGCTGTCCATGGACCGGTACCTAAACTACTTCGCCAC
  CAAAGCTAGTCCCACAGGTGTCATCTTAGACCTCTGGGAAGCTCGGCAACAGGATGACGGGGACCTCAAC
  AGCCTGGCCAGTGCCTTGGAGGAGATGGGCAAGAGTGAGATGCTGGTAGCCATGGCCACAGATGGCGATT
  GCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC057560
• Insert Size: 2805 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057560, AAH57560
• RefSeq Size: 3672 bp
• RefSeq ORF: 2804 bp
• Locus ID: 107449
• Cytogenetics: 10 31.52 cM
• Gene Summary: Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis (PubMed:15510105). Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (PubMed:15510105). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (By similarity).[UniProtKB/Swiss-Prot Function]