Zfp451 (BC062154) Mouse Untagged Clone

CAT#: MC220869

Zfp451 (untagged) - Mouse zinc finger protein 451 (cDNA clone MGC:70050 IMAGE:6331597), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp451
• Synonyms: 4930515K21Rik; 4933435G09Rik; AI596398; COASTER; Kiaa0576-hp; mKIAA1702; Znf451
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC062154
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGGTCTGAGAAGCCAATTTTGTGTCCTATAATGCACTGTAACAAGGAATTTGACAATGGGCACC
  TTCTGTTGGGACATTTGAAAAGGTTTGATCACTCCCCATGTGATCCCACAATTACGTTACATGGACCTCT
  GGCTAATTCGTTTGCATGTGCAGTGTGCTATGAACATTTCGTTACTCAACAGCAGTACAAGGACCATCTT
  CTCTCCAGGACAGCTGCAGCCGATGGACATAGCAATAGCCTTCTTCCTCAGATTATTCAATGTTATGCTT
  GTCCACAGTGCTTCCTTTTGTTTAGCACCAAGGATGAGTGTTTGAAGCATATGTCTACAAAGAATCATTT
  CCATCAGAGCTTTAAACTGAGTGATAATAAAGGAACGGCCCGCCCAATATCATTTCCATCTTTTGCAAAG
  AAGCGTTTGGTCTCTCTGTGCAAAGATGTTCCATTTCAGGTTAAGAGTGTGGCCTGCCACCAGACTCTGC
  GTTCTCACATGGAGCTCACTGCCCATTTCAGGGTTCGTTGTCAAAATGCTGGACCTGTTGCTATAGCTGA
  AAAAAGCATTACTCAGGTTGCAAAAGAATTCATAGTAAGAGGTTATTGTTCAGATTGCAACCAGGTCTTT
  ATGGATGTAGCCAGCACCCAAAGTCACAAGAATTCAGGACACAAAATTACACTTGCGAACTCGGTGGAAG
  AATCTGTCTTGCTTTATTGCCACATCAGTGAAGGGAGTCGGCCTCCTTGTGATTTACATTTATTTAGTCA
  ACCAAAAATTTCATCACTTAAACGGATTCTGTCCGTTAAAGAGTCCAGTGCAGAGGATTGTATCGTTCCG
  ACAAAGAAGGTGAACTTAGGTGTTGAAAGCCTTGGAGGTGCAACTCGTGTGCAGAGGCAGAGTCCAGCAG
  TCACAGCCTGGTTTTGTGAATGCAGACGGCAGTTTCCCAGTGAAGAGGCGGTAGAAAAGCATGTTTTCTC
  GGCAAACACAATGTGTTATAAGTGTGTGGTCTGTGGAAAGGTTTGTGAAGATTCGGGGGTCATGCGTTTA
  CACATGAGCCGGTTTCATGGAGGGGCGCATTTAAATAACTTTCTATTTTGGTGTCGGACGTGCAAAAAGG
  AGTTAGTAAAGAAAGATGCCATCATGGCACACATTACTGAGTTTCATAGTGGGCATAGATATTTTTATGA
  GATGGATGAGGTAGAGGAGGAAGAGGAGGAGGCCATGCCGTCATCCTCTGTGGAGAGCCATTTGAATACT
  GACAAACCTCCTTCACCCATTGCTGTTGTTGATCACTGCCCGGCCAACAGTCCTCCCAGGGGCAGGTGGC
  AGTGCCGCATCTGTGAGGACATGTTTGAATCCCAGGAGTGTGTGAAGCAGCACTGCATGTCCTTGACAAG
  CCACCGGTTTCACAGATACAGCTGTGCCCACTGCAGAAAGACTTTCCATAAAGTGGAAACTCTGTACCGA
  CATTGCCAGGACGAGCATGACAGTGAGATCATGATGAAGTACTTCTGTGGGCTTTGTGATCTCATTTTTA
  ACAAGGAAGAAGAATTTCTGAGTCACTATAAGGAACACCACAGCATAGATTATGTGTTTGTGTCAGAAAA
  AACTAAAACCTCAATTAAAACTGAAGGCGATTTTAAAATAGTAGAGACCAGTAGTTTACTAAGCTGTGGC
  TGTCATGAGAGTTACATGTGTAAAATCAACAGAAAGGAAGACTATGACCGATGCCTTCCAGTCCTGCTGG
  AGAAAGGTAGGCTGTGGTTTCGCTGCAGCTCGTGCTCAGCCACAGCACAGAATGTGACCGACATTAACAC
  TCACGTCTGCCAAGTGCACAGAAAAGAGAAAAGTGAGGAGGAGCAGCAGTATGTGATCAAGTGTGGCATC
  TGCACCAAGGCATTCCAGAACACGGAGAGCGCTCAGCAGCACTTCCACAGGAAGCACGCGGCCCTCCAGA
  AACCCACCGCGACCCCAGGGGGAGCCAACAGAAGCAGCACATGCCAGCTGGCTGCTAGTGCCTCACATGC
  TGAGAAAAACCTGAAACAGCCTAGCTCTCAGAAACATTCAGACGTGGAAAAAGGAGCTGAGCATGATGTA
  CGCTGCCAGAACATAGAGGAGGAAGTTGAGCTTCCGGATGTGGACTACCTGCGGACCATGACTCACATAG
  TCTTTGTAGATTTTGATAACTGGTCCAACTTTTTTGGTCATCTACCTGGGCATCTTAATCAAGGAACGTT
  TATTTGGGGCTTTCAAGGGGGAAACACCAACTGGAAGCCCCCGCTCAGCTGTAAGGTCTATAATTACTTG
  AGCAGGATTGGCTGCTTCTTCCTTCATCCTCGCTGCAGTAAAAGAAAAGATGCCGCGGATTTTGCCATAT
  GTATGCATGCTGGCCGTCTAGATGAGCAACTTCCCAAACAAATTCCTTTCACCATCCTCTCGGGAGACCA
  AGGCTTTCTGGAGCTAGAGAATCAATTTAAGAAGACCCAGAGGCCAGCTCATATACTAAACCCTCACCAC
  TTAGAGGGAGACATGATGTGTGCCTTGTTAAATAGCATATCTGATACTACCAAAGAGTGTGACAGTGACG
  ATAGCTCGGGGATGAAAGGATCTCCAGCAGAGGAGCTCAGGGCCACGGAGGATGTGGAATTAGAAGAAGC
  TATTAGAAGAAGTCTTGAGGAGATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC062154
• Insert Size: 2688 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062154, AAH62154
• RefSeq Size: 3597 bp
• RefSeq ORF: 2687 bp
• Locus ID: 98403
• Cytogenetics: 1 12.81 cM
• Gene Summary: E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins. Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation. Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11'. Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9'. Plays a role in regulating the transcription of AR targets (By similarity).[UniProtKB/Swiss-Prot Function]