Parp9 (NM_030253) Mouse Untagged Clone

CAT#: MC220862

Parp9 (untagged) - Mouse poly (ADP-ribose) polymerase family, member 9 (Parp9), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Parp9
• Synonyms: ARTD9; AW214463; Bagl; Bal; BC003281; PARP-9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220862 representing NM_030253
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTATTACATGGATACATGGGCGGCAGCTCCCGCCGAAAGACCAGCCAACAATTCTCTTGAAGAAC
  ATTATAGATGGCAAATTCCCATTAAACACAATGTCTTCGAAATTTTAAAGAGCAATGAGAGTCAGCTATG
  TGAAGTCCTCCAAAATAAGTTTGGATGCATCTCTACCCTGAGCTGTCCAACTCTAGCAGGGAGCAGCTCT
  CCTGCTCAGAGAGTCTTCAGAAGGACCCTGATCCCTGGGATAGAGTTATCTGTCTGGAAGGATGACCTTA
  CCAGACACGTTGTTGATGCTGTGGTGAACGCAGCCAATGAAAACCTTTTGCATGGAAGTGGCCTGGCCGG
  AAGCTTGGTGAAAACTGGTGGCTTTGAAATCCAAGAAGAGAGCAAAAGAATCATTGCCAACGTTGGTAAA
  ATCTCAGTTGGTGGAATCGCTATCACCGGTGCGGGGAGACTTCCTTGCCATTTGATTATCCATGCGGTTG
  GACCTCGGTGGACAGTTACGAACAGCCAGACAGCTATCGAATTACTGAAATTTGCCATTAGGAACATTCT
  AGATTATGTCACCAAATATGATCTACGCATTAAGACAGTAGCAATTCCAGCCCTGAGCTCTGGAATTTTC
  CAGTTCCCTCTGGATTTGTGTACAAGCATAATTTTAGAAACTATCCGGCTTTATTTCCAAGACAAGCAAA
  TGTTCGGTAATTTGAGAGAGATTCATCTGGTGAGCAATGAGGACCCCACTGTTGCGTCCTTTAAATCCGC
  CTCAGAAAGCATCCTAGGGAGGGACCTGAGCTCTTGGGGGGGTCCAGAAACTGACCCTGCTTCCACCATG
  ACTCTTCGCATCGGCCGGGGCCTGACTCTCCAGATTGTCCAAGGCTGTATTGAAATGCAAACAACAGATG
  TAATTGTTAATTCTGGATACATGCAGGATTTTAAATCAGGACGAGTGGCACAGTCGATTCTTAGACAAGC
  AGGGGTTGAAATGGAAAAGGAACTTGACAAGGTTAACCTGTCCACAGATTATCAAGAGGTGTGGGTCACA
  AAAGGATTTAAATTGTCCTGTCAGTATGTCTTCCATGTGGCATGGCATTCCCAAATCAACAAATACCAGA
  TATTGAAAGATGCAATGAAGTCCTGTCTAGAAAAATGCCTTAAACCAGATATAAATTCCATTTCCTTTCC
  TGCTCTCGGGACAGGATTGATGGATTTGAAGAAGAGTACAGCAGCTCAGATAATGTTTGAGGAAGTTTTT
  GCATTTGCTAAAGAGCACAAGGAAAAAACGCTAACTGTAAAGATTGTGATCTTTCCAGTAGATGTGGAGA
  CGTACAAGATTTTTTATGCTGAAATGACAAAAAGGTCCAACGAGCTGAATCTCAGCGGTAATAGTGGTGC
  TTTAGCCCTGCAGTGGTCCAGTGGGGAGCAAAGAAGAGGCGGCCTTGAAGCTGGATCTCCTGCCATCAAT
  CTCATGGGTGTAAAAGTGGGAGAGATGTGTGAGGCCCAGGAATGGATTGAAAGGTTGCTGGTCTCCCTGG
  ACCACCACATCATTGAGAATAATCATATTCTCTATCTTGGGAAAAAAGAGCACGACGTGCTGTCTGAGCT
  CCAGACCAGCACAAGAGTCTCCATTTCAGAGACTGTCAGTCCAAGAACGGCCACTTTGGAGATTAAAGGT
  CCCCAGGCTGACCTCATTGACGCAGTTATGAGGATTGAATGTATGCTGTGTGACGTTCAGGAAGAAGTGG
  CAGGAAAAAGGGAGAAAAATCTTTGGAGCTTGTCAGGACAGGGGACCAACCAGCAAGAAAAACTGGATAA
  AATGGAAGAATCGTACACATTTCAACGATACCCAGCATCATTAACTCAGGAACTTCAGGACCGAAAGAAA
  CAGTTTGAAAAGTGTGGCTTGTGGGTTGTGCAGGTGGAGCAGATAGACAATAAGGTGCTGCTGGCTGCCT
  TCCAAGAGAAGAAGAAAATGATGGAAGAGAGGACGCCAAAGGGATCTGGGAGCCAAAGGTTGTTTCAGCA
  GGTCCCACATCAGTTCTGCAATACGGTGTGCAGAGTCGGCTTCCACAGAATGTATTCGACATCCTATAAC
  CCAGTTTATGGAGCCGGCATATATTTCACCAAGAGCCTCAAAAATCTAGCAGACAAGGTCAAGAAAACCT
  CAAGCACAGACAAGCTAATCTATGTGTTTGAGGCAGAAGTACTCACAGGGTCCTTCTGTCAGGGTAATTC
  CTCAAATATCATCCCTCCACCATTGAGTCCTGGGGCCTTAGATGTCAATGACAGCGTAGTTGACAATGTT
  TCCAGCCCTGAAACCATTGTTGTTTTTAATGGCATGCAGGCCATGCCCCTGTACTTGTGGACTTGCACAC
  AGGATAGGACATTCTCACAGCATCCGATGTGGTCACAGGGCTACTCATCAGGACCAGGAATGGTCTCTTC
  GCTGCAGTCCTGGGAATGGGTCTTAAATGGCAGCTCTGTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030253
• Insert Size: 2493 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030253.3, NP_084529.1
• RefSeq Size: 3564 bp
• RefSeq ORF: 2493 bp
• Locus ID: 80285
• UniProt ID: Q8CAS9
• Cytogenetics: 16 B3
• Gene Summary: ADP-ribosyltransferase which, in association with E3 ligase DTX3L, plays a role in DNA damage repair and in immune responses including interferon-mediated antiviral defenses (PubMed:27796300). Within the complex, enhances DTX3L E3 ligase activity which is further enhanced by PARP9 binding to poly(ADP-ribose) (By similarity). In addition, positively regulates DTXL3 protein levels (By similarity). In association with DTX3L and in presence of E1 and E2 enzymes, mediates NAD(+)-dependent mono-ADP-ribosylation of ubiquitin which prevents ubiquitin conjugation to substrates such as histones (By similarity). During DNA repair, PARP1 recruits PARP9/BAL1-DTX3L complex to DNA damage sites via PARP9 binding to ribosylated PARP1 (By similarity). Subsequent PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (By similarity). In response to DNA damage, PARP9-DTX3L complex is required for efficient non-homologous end joining (NHEJ) but the complex function is restrained by PARP9 activity (By similarity). Dispensable for B-cell receptor (BCR) assembly through V(D)J recombination and class switch recombination (CSR) (PubMed:28105679). In macrophages, positively regulates pro-inflammatory cytokines production in response to IFNG stimulation by suppressing PARP14-mediated STAT1 ADP-ribosylation and thus promoting STAT1 phosphorylation (PubMed:27796300). Also suppresses PARP14-mediated STAT6 ADP-ribosylation (By similarity).[UniProtKB/Swiss-Prot Function]