Cul2 (BC026779) Mouse Untagged Clone

CAT#: MC220829

Cul2 (untagged) - Mouse cullin 2 (cDNA clone MGC:25783 IMAGE:4018298), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cul2
• Synonyms: 1300003D18Rik; 4932411N15Rik; AI327301; mKIAA4106
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026779
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTAAAACCAAGAGTAGTGGATTTTGATGAGACATGGAACAAACTCCTAACTACAATCAAAGCTG
  TTGTTATGTTGGAATATGTCGAGCGAGCAACATGGAATGACCGCTTCTCAGATATCTATGCTTTATGTGT
  GGCCTATCCTGAACCACTTGGAGAAAGACTTTATGCGGAAACAAAGATTTTTTTGGAAAGTCACGTTCGG
  CATTTGTATAAGAGAGTTCTGGAGTCTGAAGAACAAGTGCTTGTTATGTATCATAGGTACTGGGAAGAAT
  ACAGCAAAGGCGCCGACTATATGGACTGCTTATATAGGTATCTCAATACCCAGTATATTAAAAAGAATAA
  ATTGACAGAAGCTGATATACAGTATGGCTATGGTGGAGTAGATATGAATGAACCACTTATGGAAATAGGA
  GAGCTAGCATTGGATATGTGGAGGAAGTTAATGGTTGAACCCCTTCAGAACATCCTCATCCGGATGCTGC
  TTCGAGAAATTAAAAATGATCGTGGTGGTGAAGATCCAAACCAGAAAGTAATCCATGGGGTTATTAACTC
  CTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTAAAGTTTTATCAGGGAATATTCGTGTCTCCC
  TTTCTGACTGAAACGGGAGAATATTACAAGCAAGAAGCTTCAAATTTATTACAAGAATCAAATTGTTCAC
  AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTAGATGTCGAAAATATTTGCATCCAAG
  TTCATATACTAAAGTGATTCATGAGTGTCAGCAGCGGATGGTAGCAGACCACTTACAGTTCTTACATTCA
  GAGTGCCACAGCATCATTCAGCAAGAGAGGAAGAACGACATGGCAAATATGTACGTCTTACTCCGTGCTG
  TGTCCAGTGGTTTACCTCATATGATTGAGGAGCTGCAGAAACATATCCATGACGAGGGCCTCAGAGCGAC
  CAGTAACCTTACTCAGGAACATATGCCAACACTATTTGTGGAATCAGTTTTGGAAGTACATGGTAAATTC
  GTTCAACTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGCTTTAGATAAGGCCCTTACAT
  CTGTTGTAAATTACAGAGAACCCAAGTCTGTTTGCAAAGCGCCTGAACTGCTTGCTAAGTACTGTGACAA
  CTTGCTAAAGAAGTCAGCGAAGGGGATGACTGAAAATGAAGTGGAGGACAAGCTCACTAGCTTCATCACA
  GTTTTCAAGTACATTGATGACAAGGACGTCTTTCAGAAGTTCTATGCAAGAATGCTAGCAAAACGTCTAA
  TTCACGGGTTGTCTATGTCTATGGATTCTGAAGAAGCCATGATCAATAAATTAAAGCAAGCCTGTGGTTA
  TGAGTTTACCAGCAAGCTGCATCGAATGTATACAGACATGAGTGTCAGCGCTGATTTGAACAATAAGTTC
  AACAATTTCATCAGAAACCAAGACACCGTGATTGACTTGGGGATTAGTTTCCAAATATATGTTCTTCAGG
  CTGGTGCGTGGCCTCTCACTCAGGCTCCTTCATCTACATTTGCAATTCCCCAAGAGCTAGAGAAAAGTGT
  ACAGATGTTTGAATTGTTTTATAGCCAGCATTTCAGTGGACGGAAGCTGACATGGTTGCATTATCTCTGT
  ACAGGTGAAGTTAAAATGAACTATTTGGGAAAACCATACGTAGCTATGGTTACAACCTACCAAATGGCAG
  TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGCTACAAAGAACTTCAAGACAGTACCCAGATGAATGA
  AAAGGAGCTGACCAAAACAATCAAATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAA
  GATATCGATGCAGAATCTTCATTTTCATTAAATATGAGCTTCAGCAGTAAGAGAACAAAATTTAAGATTA
  CTACGTCAATGCAGAAGGATACGCCACAAGAACTGGAACAGACTAGAAGTGCTGTAGATGAGGACCGGAA
  AATGTATCTTCAAGCTGCTATTGTCCGCATCATGAAAGCACGAAAAGTGCTTCGGCACAACGCCCTCATT
  CAAGAGGTAATCAGCCAGTCAAGAGCCAGGTTCAATCCCAGTATCAGCATGATTAAGAAGTGCATAGAAG
  TTCTGATAGACAAACAGTACATCGAGCGCAGCCAAGCATCTGCAGATGAATACAGTTACGTCGCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC026779
• Insert Size: 2238 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026779, AAH26779
• RefSeq Size: 2765 bp
• RefSeq ORF: 2237 bp
• Locus ID: 71745
• Cytogenetics: 18 A1
• Gene Summary: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (By similarity). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) (By similarity).[UniProtKB/Swiss-Prot Function]